LOCUS       sequence-307722-       11369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent fusion protein for tracking Akt signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Gross SM, Rotwein P
  TITLE     Akt signaling dynamics in individual cells.
  JOURNAL   J Cell Sci. 2015 Jun 3. pii: jcs.168773.
  PUBMED    26040286
REFERENCE   2  (bases 1 to 11369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2015 Jun 3. pii: jcs.168773."
COMMENT     Sequence Label: pLenti-FoxO1-Clover
FEATURES             Location/Qualifiers
     source          1..11369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2270..3448
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2501..3439
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3396..3416
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(5486..5507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     6094..6115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6155..6179)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6210..6798
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6263..6283)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6681..6692)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6812..7311
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6847..6870)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7244..7263
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7332..7931
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7332..7351)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7828..7848
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8069..8302
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8374..8495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8411..8430)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8465..8484
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8535..8670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8690..8709)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8691..8709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8719..8736)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8719..8735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8728..8750)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8877..9332
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8964..8983)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9174..9195
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9358..9462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9463..9531,9532..10323)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9681..9700)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10494..11082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10983..11002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11236..11253
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttta
     2161 aaggaaccaa ttcagtcgac gataagcttt gcaaagatgg ataaagtttt aaacagagag
     2221 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     2281 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2341 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2401 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2461 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     2521 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     2581 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     2641 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     2701 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     2761 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     2821 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     2881 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     2941 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     3001 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     3061 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     3121 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     3181 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     3241 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     3301 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     3361 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     3421 tttttttctt ccatttcagg tgtcgtgagg aatttcgaca tttaaattta attaaaccat
     3481 ggcagaggct ccccaggtgg tggagaccga cccggacttc gagccgctgc cccggcagcg
     3541 ctcctgtacc tggccgctgc ccaggccgga gtttaaccag tccaactcga ccacctccag
     3601 tccggcgccg tcgggcggcg cggccgccaa ccccgacgcc gcggcgagcc tggcctcggc
     3661 gtccgctgtc agcaccgact ttatgagcaa cctgagcctg ctggaggaga gtgaggactt
     3721 cgcgcgggcg ccaggctgcg tggccgtggc ggcggcggct gcggccagca ggggcctgtg
     3781 cggggacttc cagggccccg aggcgggctg cgtgcaccca gcgccgccac agcccccacc
     3841 gaccgggccg ctgtcgcagc ccccacccgt gcctccctcc gctgccgccg ccgcggggcc
     3901 actcgcggga cagccgcgca agaccagctc gtcgcgccgc aacgcgtggg gcaacctgtc
     3961 gtacgccgac ctcatcacca aggccatcga gagctcagcc gagaagaggc tcaccctgtc
     4021 gcagatctac gagtggatgg tgaagagcgt gccctacttc aaggataagg gcgacagcaa
     4081 cagctcggcg ggctggaaga atgcaattcg ccgcaatcta gcccttcaca gcaagtttat
     4141 tcgagtgcag aatgaaggaa ctggaaagag ttcttggtgg atgctcaatc cagagggagg
     4201 caagagcgga aaatcacccc ggagaagagc tgcgtccatg gacaacaaca gtaaatttgc
     4261 taagagccga gggcgggctg ctaagaaaaa agcatctctc cggtctgggc aagagggtcc
     4321 tggagacagc cctgggtctc agttttctaa gtggcctgcg agtcctgggt cccacagcaa
     4381 cgatgacttt gataactgga gtacatttcg tcctcgaacc agctcaaatg ctagtaccat
     4441 cagtgggaga ctttctccca tcatgacaga gcaggatgac ctgggagatg gggacgtgca
     4501 ttccctggtg tatccaccct ctgctgccaa gatggcgtct acgctgccca gtctgtctga
     4561 aatcagcaat ccagaaaaca tggagaacct tctggataat ctcaaccttc tctcgtcccc
     4621 aacatcttta actgtgtcca ctcagtcctc gcctggcagc atgatgcagc agacaccatg
     4681 ctattcgttt gcaccgccaa acaccagtct aaattcaccc agtccaaact actcaaagta
     4741 cacatacggc caatccagca tgagcccttt gccccagatg cctatgcaga cacttcagga
     4801 cagcaaatca agttacggag gattgaacca gtataactgt gccccaggac tcttgaaaga
     4861 gttgttgact tctgactctc ctccccacaa tgacattatg tcaccggttg atcccggagt
     4921 ggcccaaccc aacagtcggg tcctgggcca aaatgtaatg atgggcccta attcggtcat
     4981 gccagcgtat ggcagccagg catctcataa caaaatgatg taccccagct cccacaccca
     5041 ccctggacat gcacagcaaa cggcttcggt caacggccgt accctgcccc atgtggtgaa
     5101 caccatgcct cacacatctg ccatgaaccg cttgaccccc gtgaagacac ctttacaagt
     5161 gcctctgtcc caccccatgc agatgagtgc cctgggcaac tactcctcgg tgagcagctg
     5221 caatggctat ggtaggatgg gtgtcctcca ccaggagaag ctcccaagtg acttggatgg
     5281 catgtttatt gagcgcttgg actgtgacat ggagtccatc attcggaatg accccatgga
     5341 tggagatacc ttggatttta actttgataa tgtgttgccc aaccaaagct tcccacacag
     5401 tgtcaagact acaacacaca gctgggtgtc aggaggatct atggtgagca agggcgagga
     5461 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5521 gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
     5581 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     5641 cggcgtggcc tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5701 cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta
     5761 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5821 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa
     5881 cagccactac gtctatatca cagccgacaa gcagaagaac agcatcaagg ctaacttcaa
     5941 gatccgccac aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     6001 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccgc
     6061 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     6121 tgccggaatc acactgggat ccctcgaggt ctagacccag ctttcttgta caaagtggtt
     6181 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     6241 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6301 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6361 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6421 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6481 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6541 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6601 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6661 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6721 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6781 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
     6841 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     6901 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6961 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     7021 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     7081 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     7141 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     7201 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     7261 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     7321 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     7381 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     7441 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     7501 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     7561 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7621 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7681 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7741 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7801 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7861 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7921 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     7981 atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8041 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     8101 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8161 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8221 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8281 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8341 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8401 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8461 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8521 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8581 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8641 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8701 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8761 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8821 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8881 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8941 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9001 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9061 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9121 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9181 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9241 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9301 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     9361 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9421 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9481 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9541 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9601 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9661 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9721 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9781 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9841 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9901 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9961 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    10021 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    10081 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    10141 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    10201 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    10261 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    10321 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    10381 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10441 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10501 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10561 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10621 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10681 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10741 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10801 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10861 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10921 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10981 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    11041 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    11101 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    11161 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    11221 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    11281 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    11341 tggaaagcgg gcagtgagcg caacgcaat
//
