LOCUS       sequence-307725-        1885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hu CCDC47 -flag mammalian expression Gateway vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pEXPqcxip-hCCDC47-FLAG
FEATURES             Location/Qualifiers
     source          1..1885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..112
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     63..83
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     109..133
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    300..324
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1783..1807)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1809..1832
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
       61 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      121 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      181 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acggacctgc aggaattgat
      241 ccgcggccgc accggtaggc ctcgtacgct taattaacgg atcggagcgg ccgctcttca
      301 caagtttgta caaaaaagca ggctccacca tgaaagcctt ccacactttc tgtgttgtcc
      361 ttctggtgtt tgggagtgtc tctgaagcca agtttgatga ttttgaggat gaggaggaca
      421 tagtagagta tgatgataat gacttcgctg aatttgagga tgtcatggaa gactctgtta
      481 ctgaatctcc tcaacgggtc ataatcactg aagatgatga agatgagacc actgtggagt
      541 tggaagggca ggatgaaaac caagaaggag attttgaaga tgcagatacc caggagggag
      601 atactgagag tgaaccatat gatgatgaag aatttgaagg ttatgaagac aaaccagata
      661 cttcttctag caaaaataaa gacccaataa cgattgttga tgttcctgca cacctccaga
      721 acagctggga gagttattat ctagaaattt tgatggtgac tggtctgctt gcttatatca
      781 tgaattacat cattgggaag aataaaaaca gtcgccttgc acaggcctgg tttaacactc
      841 atagggagct tttggagagc aactttactt tagtggggga tgatggaact aacaaagaag
      901 ccacaagcac aggaaagttg aaccaggaga atgagcacat ctataacctg tggtgttctg
      961 gtcgagtgtg ctgtgagggc atgcttatcc agctgaggtt cctcaagaga caagacttac
     1021 tgaatgtcct ggcccggatg atgaggccag tgagtgatca agtgcaaata aaagtaacca
     1081 tgaatgatga agacatggat acctacgtat ttgctgttgg cacacggaaa gccttggtgc
     1141 gactacagaa agagatgcag gatttgagtg agttttgtag tgataaacct aagtctggag
     1201 caaagtatgg actgccggac tctttggcca tcctgtcaga gatgggagaa gtcacagacg
     1261 gaatgatgga tacaaagatg gttcactttc ttacacacta tgctgacaag attgaatctg
     1321 ttcatttttc agaccagttc tctggtccaa aaattatgca agaggaaggt cagcctttaa
     1381 agctacctga cactaagagg acactgttgt ttacatttaa tgtgcctggc tcaggtaaca
     1441 cttacccaaa ggatatggag gcactgctac ccctgatgaa catggtgatt tattctattg
     1501 ataaagccaa aaagttccga ctcaacagag aaggcaaaca aaaagcagat aagaaccgtg
     1561 cccgagtaga agagaacttc ttgaaactga cacatgtgca aagacaggaa gcagcacagt
     1621 ctcggcggga ggagaaaaaa agagcagaga aggagcgaat catgaatgag gaagatcctg
     1681 agaaacagcg caggctggag gaggctgcat tgaggcgtga gcaaaagaag ttggaaaaga
     1741 agcaaatgaa aatgaaacaa atcaaagtga aagccatgtt ggacccagct ttcttgtaca
     1801 aagtggtgga ttacaaggat gacgacgata agtgattcga aggatcctcc gatccggaat
     1861 tccgcccctc tccctccccc ccccc
//
