LOCUS       sequence-307776-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with sfGFP under KanMX 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP272
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(43..45,46..759)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             43..756
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(73..101)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     696..716
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      796..983
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(851..875)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatcgg tgacggtgct ggtttaatta acatgagtaa aggagaagaa
       61 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      121 ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg gaaaactcac ccttaaattt
      181 atttgcacta ctggaaaact acctgttccg tggccaacac ttgtcactac tctgacctat
      241 ggtgttcaat gcttttcccg ttatccggat cacatgaaac ggcatgactt tttcaagagt
      301 gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga cgggacctac
      361 aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat cgagttaaag
      421 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aactcgagta caactttaac
      481 tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa
      541 attcgccaca acgttgaaga tggttccgtt caactagcag accattatca acaaaatact
      601 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac acaatctgtc
      661 ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct
      721 gctgggatta cacatggcat ggatgagctc tacaaataat aagcttggct gttttggcgc
      781 gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
      841 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
      901 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct
      961 cttattgacc acacctctac cggcagatcc gctagggata acagggtaat atagatctgt
     1021 ttagcttgcc tcgtccccgc cgggtcmccc ggccagcgac atggaggccc agaataccct
     1081 ccttgacagt cttgacgtgc scagctcagg ggcatgatgk gactgtcgcc cgta
//
