LOCUS       sequence-307782-        1168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast codon 
            optimized mCardinal under KanMX selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1168)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP1999
FEATURES             Location/Qualifiers
     source          1..1168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      789..976
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(844..868)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cgacggatcg gtgacggtgc tggtttaatt aacatggttt ccaagggtga agaattgatc
       61 aaagaaaaca tgcacatgaa gttgtacatg gaaggtactg ttaacaacca tcatttcaag
      121 tgtactaccg aaggtgaagg taaaccatat gaaggtactc aaacccaaag aatcaaggtt
      181 gttgaaggtg gtccattgcc atttgctttt gatattttgg ctacctgctt catgtacggt
      241 tctaagactt tcattaacca cacccaaggt atcccagatt tcttcaaaca atctttccca
      301 gaaggtttca cttgggaaag agttactact tacgaagatg gtggtgtttt gactgttact
      361 caagatacct ctttacaaga cggttgcttg atctacaacg ttaagttgag aggtgttaac
      421 ttcccatcta atggtccagt catgcaaaaa aagactttgg gttgggaagc tactactgaa
      481 acattatatc cagctgatgg tggtttggaa ggtagatgtg atatggcttt gaaattggtt
      541 ggtggtggtc acttgcattg caacttgaaa actacttaca gatccaagaa gccagccaag
      601 aatttgaaaa tgccaggtgt ttacttcgtc gatagaagat tggaaagaat caaagaagcc
      661 gacaacgaaa cctacgttga acaacacgaa gttgctgttg ctagatattg tgatttgcca
      721 tctaagttgg gtcacaagtt gaatggtatg gacgaattat acaagtaagg cgcgccactt
      781 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
      841 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
      901 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg
      961 accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc tgtttagctt
     1021 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgam
     1081 agtcttgacs tgcscagctc aggggcatga tgtgactgtc gcccgtacat ttagcccawa
     1141 catccccatg gawaatcatt tgcatcca
//
