LOCUS       sequence-307784-        1153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast codon 
            optimized mNeptune2 under KanMX selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1153)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP2000
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      792..979
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(847..871)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ggtcgacgga tcggtgacgg tgctggttta attaacatgg tttccaaggg tgaagaattg
       61 atcaaagaaa acatgcacat gaagttgtac atggaaggta ctgttaacaa ccatcatttc
      121 aagtgtacct ctgaaggtga aggtaaacca tatgaaggta ctcaaactgg tagaatcaag
      181 gttgttgaag gtggtccatt gccatttgct tttgatattt tggctacctg cttcatgtac
      241 ggttctaaga ctttcattaa ccacacccaa ggtatcccag atttcttcaa acaatctttc
      301 ccagaaggtt tcacttggga aagagttact acttacgaag atggtggtgt tttgactgtt
      361 actcaagata cctctttaca agacggttgc ttgatctaca acgttaagtt gagaggtgtt
      421 aacttcccat ctaatggtcc agtcatgcaa aaaaagactt tgggttggga agctagtact
      481 gaaacattat atccagctga tggtggtttg gaaggtagat gtgatatggc tttgaaattg
      541 gttggtggtg gtcacttgca ttgcaacttg aaaactactt acagatccaa gaagccagcc
      601 aagaatttga aaatgccagg tgtttacttc gtcgatagaa gattggaaag aatcaaagaa
      661 gccgacaacg aaacctacgt tgaacaacac gaagttgctg ttgctagata ttgtgatttg
      721 ccatctaagt tgggtcacaa gttgaatggt atggacgaat tatacaagta aggcgcgcca
      781 cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
      841 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
      901 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta
      961 ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatag atctgtttag
     1021 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
     1081 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcccccgta cwtttagccc
     1141 atacatcccc atg
//
