LOCUS       sequence-307786-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast codon 
            optimized mNeongreen under KanMX selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP1975
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(870..888)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(871..888)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(969..988)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaatttgtat acacttattt tttttataac ttatttaata ataaaaatca taaatcataa
       61 gaaattcgct tatttagaag tggcgcgcct tacttgtata attcatccat acccataaca
      121 tcggtgaagg ccttttgcca ttctttgaaa ttcaattcag ttttggagtg cttcaattcg
      181 gtctttctga aaacgtacat aggttgattc ttcaagtagt tagcagccat aggtttagcg
      241 aaagtgtaag tagttctagc agtggatcta tatctcttac cgttaccagt agtgtaagac
      301 cacttgaaag tagagatgat ggtcttatcg tttgggtaag tttttttgga tctgcaccaa
      361 tcagcagcag tcaaagaatt agtcataact ggaccatcag ctggaaaacc agtaccctta
      421 acttgagctt cacctttgat gtgggaacct tcgtaagtat atctgtagtt gacggtcaaa
      481 gaagcaccat cttcaaattg catggttcta tgaacttggt aaccagaacc atcaaccata
      541 gcagcttgaa atggagacat accatctgga tatggcaaat attggtggaa accgtaacca
      601 atatgtggaa ccaaaatcca tggagagaat tgcaagtcac cttttgtgga cttcaagttc
      661 aattcttcgt aaccatcatt tggattacca gtaccttgac caaccatatc gaaatcaaca
      721 ccgttgatgg aaccgaaaat atgcaattca tgagtagctg gcaaagaagc catgttatct
      781 tcttcaccct tggaaaccat gttaattaaa ccagcaccgt caccgatccg tcgacctgca
      841 gcgtacgaag cttcagctgg cggccgcgtt ctatagtgtc acctaaatcg tatgtgtatg
      901 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgtc catatggtgc
      961 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca ccnggcaaaa
     1021 cccgctgamg cgccctgacg ggcttgtctg stcccggca
//
