LOCUS       sequence-307790-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast codon 
            optimized mKOkappa under KanMX selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP2008
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      709..896
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(764..788)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcgacggatc ggtgacggtg ctggtttaat taacatgtcc gtcatcaagc cagaaatgaa
       61 gatgagatat tacatggacg gttccgttaa cggtcatgaa ttcactattg aaggtgaagg
      121 tactggtaga ccatatgaag gtcatcaaga aatgactttg agagttacta tggctgaagg
      181 tggtccaatg ccatttgctt ttgatttggt ttctcacgtt ttctgctacg gtcatagagt
      241 ttttactaag tacccagaag aaatcccaga ctactttaag caagcttttc cagaaggttt
      301 gtcctgggaa agatctttgg aatttgaaga tggtggttcc gcttctgttt ctgctcatat
      361 ttctttgaga ggtaacacct tctaccacaa gtctaagttt actggtgtta actttccagc
      421 cgatggtcca attatgcaaa atcaatctgt tgactgggaa ccatccactg aaaagattac
      481 tgcttctgat ggtgttttga agggtgatgt tactatgtac ttgaagttgg aaggtggtgg
      541 taaccataag tgtcaattca agactactta caaggccgcc aaagaaattt tggaaatgcc
      601 aggtgatcat tacatcggtc atagattggt tagaaagacc gaaggtaaca tcaccgaaca
      661 agttgaagat gctgttgctc attcttaagg cgcgccactt ctaaataagc gaatttctta
      721 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
      781 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
      841 aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga
      901 tccgctaggg ataacagggt aatatagatc tgtttagctt gcctcgtccc cgccgggtca
      961 cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc
     1021 aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg tawaatcatt
     1081 tgcatccata cattttgatg gccgcacggc gcgaagcaaa attacggctc ctcgctgcan
     1141 actg
//
