LOCUS       sequence-307792-        1153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast codon 
            optimized tSapphire under KanMX selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1153)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP2010
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      774..961
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(829..853)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ggtcgacgga tcggtgacgg tgctggttta attaacatgt ccaagggtga agaattattc
       61 accggtgttg ttccaatctt ggttgaattg gatggtgatg ttaacggtca caagttttct
      121 gtttctggtg aaggtgaagg tgatgctact tatggtaaat tgaccttgaa gttcatctgt
      181 accactggta aattgccagt tccatggcca actttggtta ctactttttc ttacggtgtt
      241 atggttttcg ccagataccc agatcatatg aagcaacatg atttcttcaa gtccgctatg
      301 ccagaaggtt acgttcaaga aagaaccatc ttcttcaagg atgacggtaa ctacaaaact
      361 agagccgaag ttaagttcga aggtgatact ttggttaaca gaatcgaatt gaagggtatc
      421 gacttcaaag aagatggtaa catcttgggt cacaagttgg agtacaactt caactcccat
      481 aacgtttaca tcatggccga taagcaaaag aacggtatta aggccaactt caagatcaga
      541 cacaacattg aagatggtgg tgttcaattg gctgatcact atcaacaaaa caccccaatt
      601 ggtgatggtc cagttttgtt gccagataac cattacttgt ccattcaatc cgctttgtct
      661 aaggacccaa acgaaaaaag agatcacatg gtcttgttgg aattcgttac tgctgctggt
      721 attactcatg gtatggacga attatacaag taaggcgcgc cacttctaaa taagcgaatt
      781 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
      841 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
      901 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
      961 gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc gtcnncgccg
     1021 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
     1081 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtnnaa
     1141 tcatttgcat cca
//
