LOCUS       sequence-307793-       15588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211, 
            alpha-L-Galp_697, alpha-L-Araf_1120 and beta-Xylp_1710 as inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15588)
  TITLE     Expression of arabinoxylan debranching enzymes in 
            Thermoanaerobacterium thermosaccharolyticum- Bacterial expression 
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLL1275
FEATURES             Location/Qualifiers
     source          1..15588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1422..2216)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      11882..11909
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(11927..11943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11927..11943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11940..11962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11951..11967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11975..11981,11982..11999,12000..12005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12020..12041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12235..12823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12315..12334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12994..13785,13786..13854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPEMLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13617..13636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13855..13959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14027..14045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14083..14105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14205..14224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14418..14440
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14433..14449
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             15279..15287
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctcgtgatta ttgtaaaaat gttttagata atataaagtt caatgaaaaa ataattgatt
       61 attatgttat gttacaaaat gatgttttta ttgattttac taataaaata aattcaataa
      121 gggattgtaa taaatattgg tatttggatg tttataaaaa gcagaaaata aaggatttta
      181 aaaagactaa tttgtgtaaa gataagttct gtaataattg taagaaagtt aaacaggctt
      241 caagaatgca aaaatatatt cctgaattac agaaatacaa agatggctta tatcatttta
      301 tatttactgt tgaacatgtg ccaggtagtg aattaagaga tactattgat aggttgttta
      361 agtcttttaa gtcatttaca aggtatttaa gtggtaatct taaaataaaa ggtgttaatt
      421 ttgataaatg gggttataaa ggctgtgtaa ggtctttaga ggtaacttat agtatgattg
      481 ataatcatat tatgtatcat ccacacttgc gtgttgcgat gatattagat cctttttacg
      541 atggttttaa tgttgaaagg atgcatataa ttaataagtt tagttatagc tatggtgttt
      601 taaaaaggtt gtttactgat gatgaattat taattcaaaa aatttggtat ttattgttta
      661 ataatattga ggttaacatg gccaatataa ataatttaga ggatggttat tcttgtttag
      721 ttaataagtt tagtgattat gattatgcgg agctgtttaa gtatatttgt aaaaatactg
      781 atgaacaagg tttacttatg acttatgata tttttaaaga tttatatttt gcattacata
      841 atgttcatca gatacaaggc tatggttgtt tatataatat aagagatgat actcaattag
      901 atttaaaggt tgatgacatt tataatgatt tgattgattt attacaagtt acagaaaatc
      961 ctatacagtc tatggaaact gtacaggatt tattaaagga tactgaatat acaataataa
     1021 gccgtaagcg tatatttaag tatctaacac aattatatca taaggattga tatttatacc
     1081 gtctgtcgga ctcatgcgga gggggacttg agggggtctc ccctcgcatt gtacgacaga
     1141 cggtattatt attatacaaa ttttttttat gtaatttttt tttgtgtaat ttttttatac
     1201 aaataatatt tcaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat
     1261 gcacgataca aattcctcgt aggcgctcgg gacccctatc tagcgaactt ttagaaaaga
     1321 tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta gatgtctaaa
     1381 aagcttgtag ttaaagcttt ttagacatct aaatctaggt gctaaaacaa ttcatccagt
     1441 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
     1501 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
     1561 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
     1621 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
     1681 ttttccgtct ttaaaaaatc gtacagctcg cgcggatctt taaatggagt gtcttcttcc
     1741 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
     1801 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
     1861 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
     1921 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
     1981 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
     2041 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
     2101 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
     2161 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
     2221 tatttccttc cccttttcta cagtatttaa agatacccca agaagctaat tataacaaga
     2281 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
     2341 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
     2401 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
     2461 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
     2521 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
     2581 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
     2641 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
     2701 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttgag tcgtgactaa
     2761 gaacgtcaaa gtaattaaca atacagctat ttttctcatg cctttacccc tttcataaaa
     2821 tttaatttta tcgttatcat aaaaaattat agacgttata ttgcttgccg ggatatagtg
     2881 ctgggcattc gttggtgcaa aatgttcgga gtaaggtgga tattgatttg catattgatc
     2941 tattgcattg aaatgattag ttatccgtaa atattaatta atcatatcat aaattaatta
     3001 tatcataatt gttttgacga atgaaggttt ttggataaat tatcaagtaa aggaacgcta
     3061 aaaattttgg cgtaaaatat caaaatgacc acttggatta atatggtaaa gtagatataa
     3121 tattttggta aacatgcctt cagcaaggtt agattagctg tttccgtata aattaaccgt
     3181 atggtaaaac ggcagtcaga aaaataagtc ataagattcc gttatgaaaa tatacttcgg
     3241 tagttaataa taagagatat gaggtgagag atacaagata agagatataa ggtacgaatg
     3301 tataagatgg tgcttttagg cacactaaat aaaaaacaaa taaacgaaaa ttttaaggag
     3361 gacgaaagat ggcaaagata acgattctgg aagacaagat aatttttgaa agaagagatg
     3421 agcttacggt aatagaagct tatggagcaa attgtctgcg ttaccgttca acccgcaata
     3481 ttaagctgtc tgatgagaac tggaccctgc tgccgccgac tacaaaagct gattgtaaaa
     3541 cctttggaaa tgaaagagaa gcaacgatag taaacggcga tatttccgca actataaggg
     3601 acggctttgg ctggaccgga gccagtataa gctactttcg caaggggaag cagattcttc
     3661 gatcccgtga tgagggtgat ccggttaccc gctttaccca cctgggcggt aacaattatc
     3721 aggtgaaggc gatatttgac agtaatccgg gcgagcatat atatggcctg ggacaggagc
     3781 aggaggatca gtttgaccgc aagggctgca cctgcaatat catgcattac aataccaagt
     3841 cgactttgcc ggttatttat tcgtctctgg gatatggctt tttgtggaac aatcctgcac
     3901 ccggccgttg tgagactacc tttaatcata caatgtgggt tgcggatagt gcatatcaga
     3961 ttgattatct tgtatatgtc ggggatactc cggctgacat aatgaaaata tactgtgatc
     4021 tgacaggcta tgccccgaaa ttccctgact gggcggccgg cttctggcag tcaaagctcc
     4081 gttatgaaag ccaggaggat ttgctggagg tggcaaggga atacaagaaa agaggaattc
     4141 ctctcgcagc gatagtaata gattatttcc actggacgga gcagggggaa tggaaatttg
     4201 atcccaattt gtggcctgat cccaaggcaa tgtgtgacga gcttgagtca atgggagtaa
     4261 agcccgttgt atccatctgg cctaccatta atcccaagag tgagaattat catgcaatgg
     4321 ataatgagaa tatgctggtg aggaccgaga acgggcagta tggcatattc aacttttacg
     4381 gcttacagac ctttatagat cccgccaatc ctaaaacagg gccctttgta tggagcaagg
     4441 taagggagaa ttattataag tacggaatta agaacttttg gctggatgag gctgagccgg
     4501 aggtgcatcc tcagcagttt ggcaatctgc gtttttatat aggtaacgga gcccagacgg
     4561 cattattgta tccatattat tatgcaaaga tattttatga tggcttaaga agtgagggag
     4621 aagaggatat tataatcctt acccgggcag cctatcccgg cagccaaaga tttggttccc
     4681 tggtatggaa cggagacatc ccctccacct tcaaggctct tcgccagagt gtaaccagcg
     4741 gcctttccat gtcaatgtgc ggaatcccat ggtggaacag cgacataggc ggcttccatt
     4801 ccgcagatat agaaagcgac tatttcaggg agcttatagt cagatggttc cagttcggcc
     4861 tctttagtcc ggttatgcgc cttcacgggg ccagaaaccg tacaaaaaac caggttgacc
     4921 gccatccggg cgtgaaggag agaagcggcg gagacaatga aatctggagc ttcggtgaaa
     4981 gaaattatcc tattcttaag aatattatag agcttaggga gaggctgaag ccttatattc
     5041 tcaaatatat ggatatcgcc agcaagaccg gctcaccgat tatgaggccg atgttctatg
     5101 atttttatga tgacgagatt tgctaccgac ttgatgatca gtacatgttc ggagaggata
     5161 tactcttcgc tcccattctg gagcagggag tgacacagcg cagtgtctac ctgccggagg
     5221 gtaaatgggt taatgtaaat gataaaaaaa cctacgaggg caggcaaatg ataactgcaa
     5281 gggcagagct gcatgagttt attgccttcg taagaagcgg cagcgaggtg ctggaggtat
     5341 ttagctaatt ctaaatagga ggtgtaaagt atgctggaaa tattaagatc aaacattaag
     5401 agtaggggga ttatcatgaa atactatgta gatgctaatg ctgcaaaaga cggcaatggc
     5461 tcacttgaga agccatttaa gcgaattagc gatgcagcga agctggcgct tcccggcgat
     5521 gaggtgttgg tggcgcctgg ggtttaccgg gaatatgtag atccacttaa tgcaggcact
     5581 gaggatgccc ggatcagtta tatcagcact acagctcttg gtgcagtgat taccggtgcc
     5641 gaacaggtaa agagctggac acattataag gataatgtat gggtatgcaa aatctcaaac
     5701 agtattttcg gtgattacaa tccatataca accctggtat ttggagactg gtattttgca
     5761 acaccaaata agcacaccgg ctgcgtattc ctgaatgata aggctatgta tgaagcagct
     5821 agtttggagg aatgcatcaa gggtaatatt tatgagtaca gctgggtacc ggaagactct
     5881 atatataagt ggtacaccga acaggatacg gaaaaagacg agacaatcat ttatgccaat
     5941 tttcagggac tggaccccaa taaggaaaat gtagaaataa ctgttcgtcg cagatgcttt
     6001 atgccatcca aaacaggtgt cggttatatt acggtcagag gctttaaaat cgacaaggca
     6061 gctacaacct gggcaccgcc tgcagccttt caggatggta tgatcggtcc tcactggagc
     6121 aaaggctgga ttatcgagga ttgcgagatc tcaaacagca agtgcgcagg catttctttg
     6181 ggtaaatatc ttgacacgga caatgagcat tatttcacat ataagcatgt aaaaagcgcc
     6241 acacaaatgg aacgtgatgc tgtctgccgc ggccagtatc atggctggct taaggaaaaa
     6301 atcggcggcc atatagttcg ccgctgcaac atccaccatt gtgagcaggg aggtattatc
     6361 ggccgaatgg gcggtgtgtt cagcatcata gaggacaacc atatccacca tatcaataat
     6421 atgatggagc ttggcggagc agaaatctca ggaattaagc tgcatgctgc aattgatgtg
     6481 ctcattcggc gtaaccatat ccaccattgt acaatgggaa tatggtgtga ctgggaagca
     6541 cagggtaccc gcattagcca gaacctgctc catgataacc agaggcctcc gtttgccaag
     6601 aatctaccgg gcagcatgat gtcccaggat ctgtttgtgg aagtcagcca tggacctact
     6661 cttatcgaca acaatatcct gctttcggat gtcagcctcc gctttgcaac acaaggtgtg
     6721 gctatggttc acaatcttat ctgcggctcc tttaccagtg tcggtggtgg aaccacatgg
     6781 cgctatacac cataccatat cccccaccgt accgaggtca tgggctttat gaccattctt
     6841 catggtgaca atcgtttcta caacaatatc ttcatccaga aatggcctaa tgaagaatat
     6901 accacctatg acgatcagga tgcgcagaat gcctttaggg aaaacagaca ggttggcact
     6961 catgtatttg atgaatatcc aagctatgat gaatggattt cccagttcgg tttttcacat
     7021 tatcccaata tgagagccct ggagcctgtt cattttggcc atctgccaat ctggagcgat
     7081 ggaaatgtgt atctgaacgg tgccaaagcg tggaaaaagg aagcaaacag cctggtaatt
     7141 gaaaaaacgg atcaggagct taaggtggag ctggtggaaa aggacggcca gtattatctg
     7201 aacactaata tttttgatta catcaaggat ttcaatgccc gtatgataaa cacagaaata
     7261 ctcggcaaag cctttgagcc ggagcagtac tttgaaaatg cggacggtac gccaattcgc
     7321 tttgactcag acttcttcgg aaatcatcgt ggggttcatg tattggcagg cccgtttgca
     7381 tctgcctctg aaaatataaa gctataagat agcgcatgga ggataaaaaa tgatgaagga
     7441 agatagatat gtgctgtgct atacacgaga gccacaggag gatattattt attccaacaa
     7501 gctggcttac agtatgcatc tggcatatag cgaaaatgga gtaaaatatg agccacttaa
     7561 ccacaattct ggagttttgt ttgcaaaagc cactcataat ccggatggta cgatgacagc
     7621 aaaaagcctg aagtgcccgt ttttgtttta catggaggac aatacctttg gtgttgttgc
     7681 aataaggacg gaggctaacg gagattatga tccggaatgc aggggcaagg tattgttctt
     7741 tacctcaaag gatttgattc agtacaccga ggttggcctg atagatctta atacgaatgc
     7801 atatatcagg gatgttacct gtaaatatga caaggttaag gaaagataca taatatgctg
     7861 gcgtgacgat tacggtaagt ggtatcagag tgaaatagat gatttatttt ctcttaagtg
     7921 ccagtctgaa cccgtcagca taggagcagt tggctttgcc agggttaata ctgaaataga
     7981 aggaatagta ccccgcaata ccataaaggt atctgaagaa atcggataca gattaaagag
     8041 caagctgaca acaccccgca tggtcaaaat cgagctgcct gaaaccttaa gtgcttcttc
     8101 acctgaagag ctgagcaaaa tgagatttaa agccatttac agtgacggta ccagctcgat
     8161 caagtctata gactggaata ctgagagggt tgattggagc aggcccggca cctatgatat
     8221 aaagggtgta attcatcagg atcattttga attccccatt gccatcaacc gtgctgatcc
     8281 ctgcatccgc aaatggaatg gcaagtatta ttttgtggca actaatgacg ccgacggcaa
     8341 tcacaccata tatatcaggg aagctgatac tattccggga cttgcaactg ctgaggagca
     8401 cttaattctt gactcctcca cctatgaagg tatcggtggt ctgttatggg ctccggagct
     8461 tcatgttata ggagaggatt tgtatatctt ccatgcagca acaccgggag aattcttcca
     8521 tgaggaatcc catgtaatgc aactgaagcg tggcggcaat cctctttgca aggaggattg
     8581 gtcggcaccg aagagagtag ttaaaaagga tggttcttac ctgtgtgagg ccggcaaggt
     8641 tatatcactt gatatgacct gctttgagtg gaataaggaa tactatgttg cctggtcaca
     8701 aagacagttc ctgccagttg atcagggagc ttggctctat atagccaaaa ttgatcctca
     8761 ggagccatgg agattaatca ccgatcctgt tctgctcagc aagcctgaat atggttgggc
     8821 aaataatcat acctttgttg atgaaggacc ttttgccctg ataacagata aaaaaatctt
     8881 tcttaccttc tccagcgctg cggttgatgc cacctataca gttggcctgc tggtggctga
     8941 gccgggttct gatttgctgg atcccgcaag ctggacaaag ctgaattatc ctttactgac
     9001 cagcagaagc gtacctggtc aattcggacc gggacataac tcctatgtaa ttgatgatga
     9061 tggcattgta tggaatgctt accatgccag accaggagtt aacggtccca gaagctcagg
     9121 aataagacgc gttcactttg acatagacgg atacccggtg cttgatctta ctgaggaaag
     9181 agacgtaagc gcccgtctga agaatgtatc ctgcaggctt atagtaaaat agaaattaat
     9241 gtaaggagga aaagaattgg caagagataa cacatatgaa agaataagtt ctcgaaaaat
     9301 atcaggtgaa agggctacag taatggaaat agtaaggatt aagagcagat actcatatat
     9361 ggacatatcg aagcgcagag aaatgatttc cgatagaagc gttatttcca ctgcgcctgc
     9421 aactgacagg gctgactatt tttatacggg aaacgggcac tactggctgg agagcgccgg
     9481 aggagtagag aaggacacga ttattgtaac cgaggagcat ctttatgagc ctctttggag
     9541 caaaacgcct aatccgcccg atttgcgcgg atgtattgat gatatccgca ggtgcgtcct
     9601 ggagggaaat cccgaaaagg cagatgagta tattgacagg gcacagcgca tggccggtta
     9661 cgataagctc atgaacatgg acagcaaaat tgtctacccc atcggctcac ccagagtgca
     9721 caaggccttc agctttgatg tcacaagaga aggactagat acggacagtg tatataatta
     9781 tataagatgg ctcgatatgg agaccgggct tattacgaac cactttgaga tagcgggtga
     9841 aagctatgaa agcaggatag cagcatcgtt tgccgacaat ttctctgctt ttgaatttac
     9901 cgcacccaga ggcaagctta atatctctct cgaaataaaa ctgcccggcg ggccgaatat
     9961 gttcggtgtc ccgaccatgg tcggttcgtc tcacaaatgg actgttaagg aaaacaggct
    10021 tatcctcagc tggcaatata atcccgaact gacaagcaag gggtattatg ccattatcga
    10081 cgcctcatgt gaggacggag agctaagcat cagcagtgag aaaatagaaa cgaagaattc
    10141 gacccgactg tatattgccg ttaaggtcat taagatagag gataatttcg actttgatgg
    10201 atataaggtc ttcgaggctg agtttgataa gacagacaaa aggcttggca ggcttttaca
    10261 ggggaatgcg gccatcctcg gggagaagat gaaccgctca tatattactc tgggcaagaa
    10321 ggaagactgc ttactttcgg gcgaggagct ccttgagaaa tgtcacagtg agaaggagct
    10381 ttatccaatg atgatggata agctgtacga catgggaagg ttctaccaga ttgttgatac
    10441 gggcgacatt ccgcctatgt ggggacagca caacatcaat accaatctcc aggtatgcgc
    10501 aggcaacaat acaggtctct ttgacgaaat ggatgtttac ttcaggtact atgaaagcaa
    10561 gtttgatgac ttcagaacca atgccaggct tctctttaat gcgagagggc ttctggcaag
    10621 tgttcactgt gacccggatt cgggcctgct ctatcatttt tcaaagactt atccgcatta
    10681 ctgctggaca gggtgtctgg gctggatata taacgaattc tggggatact atcttgttac
    10741 aggagataag aactttctgg agaataggat tatcccggca cttaaggaaa ttgcactgtt
    10801 ctttgaggat tacgcatgcc ttaaggatga gaagggaaag agcatatttg ttccttcgtt
    10861 ctcgcctgag gatcccactc ctaatcctga ttactcaaca gttacctgca aggacattca
    10921 tcccaccgga atcaactcgg tgatggatat cgcaatatgc cgcgaggtgc tcacaaacct
    10981 cattgaaggc tgcaaaatac tcggcataga ggaaaaaaaa tattccaaag tggaaaagac
    11041 agctggagga gcttcccgta taccttaccg atgaggaagg cggccttaag gagtgggcat
    11101 gggagtcgat agaggagaac tataatcacc gacatgtttc tcatcattac gatatatggc
    11161 caggaagagc cgtatctgtg gatagggagc ccgagctggc acgcgcaata aagatttcta
    11221 acagaaagcg cgcccagcag gacgattccg cacacggaat cattcacggg gccttctgtg
    11281 cgttaagact gaaggataag gaagaattta tccagaacat gtcgcagctg attaatcacg
    11341 gctttgtaag aagaaacctt gcaacggcac atttccctta cagaggtcaa ttccccgatc
    11401 ttcagggcgc aatgcctgct cttctgattg agatgtgcgt ttactcagag cccggatttg
    11461 tggagttctt gccggctctt cccgatgatt tcgcgcaggg caggcttgtc ggggtgtggc
    11521 tctatacctt tgcaaagttg gaaagcatgg agtggagcga aaaggaaatc aaggtatcca
    11581 taacgtccaa cagggatcag accatcacgt ttatgttaag aaacagagcg gcaaaattct
    11641 ttgtaaatgg aaaagaatat tatccctgtg acggaaaaat cgactaccac tttagcgagg
    11701 gcgaaaaagc tgatatctat attgagctgt gactgggcct ttcgttttat ctgttgtttg
    11761 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
    11821 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
    11881 cagaaggcca tcctgacgga tggcctttta gcttggcgta atcatggtca tagctgtttc
    11941 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    12001 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12061 ccgctttcca gtcgggaaac ctgtcgtgcc agcagatctg cggcgagcgg tatcagctca
    12121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    12181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    12241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    12301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    12361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    12421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    12481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    12541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    13021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    13081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    13141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    13201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    13261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    13321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    13381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    13441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    13501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    13561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    13621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    13681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    13741 caactgatct tcagcatctt ttactttcac cagcatttct gggtgagcaa aaacaggaag
    13801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    14041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    14101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    14161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    14221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    14281 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    14341 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    14401 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgacaaa
    14461 gttttctatt tgtgttaaca ttgtttatat aatagtgaac agtgttaaga ttaaatgtga
    14521 ggtgtttgta tggatattaa tgattataaa gagaagggac tttatttatt aagtagtatg
    14581 gatgatttta ttaaaattaa tgatttgttt atgggtaaag ttgtttctcc tggctatgtt
    14641 gcttcggttt ttggtgtttc caggtctact gttacacaat ggattcaaag acgtaaaatt
    14701 agagctttta agtataaagg taaggaaggt gactatatgg ttatacctat tgctgatatt
    14761 attgattaca aaagattgag taataatgat tttatttatg ataagttagt gaggtgattt
    14821 attttatgtt tgacgatagc tatgttgtta atgagtgttc gtctaatgtt agtgaaaatg
    14881 atagagattt ttgtagtttg gttggtcgtt ttatgattat taatggtata gataagttgg
    14941 ttattaagat taatagaaaa tttaatagga aatctttaag tttagatttt agtgttgatt
    15001 tattcccttc tatcaaagtt tctgaattag ttttttttga tgagtttaac aaaacgtgtg
    15061 gtttttattt ttcttttaat tcttttacaa tttttaaggc ttttagagat gttcataatc
    15121 ataataaaat atcattttat tttgcataat ttcgggtctg ggccgcagac caggcccagt
    15181 gctaacaata ttaattttta atgttaggaa ttgtttaatt cttaattgtg tttttaaagg
    15241 tagaataatt acccattcgc cctttagcca acaaaaatta aggaggtata aacatggata
    15301 aaatggattt gattcttcaa gatgaaagac tgggtgagat atttaaagat atagatttaa
    15361 cagataatga aaagagatat cttaaatggt tatggaaatg ggattatgaa acacgtgata
    15421 cttttgtatc aatttttttg aagctaaaaa atggtggaaa atgatttttt tcttatcttg
    15481 atatattaga aaaaagcgta ctcacgaagt aagaatttgt aaaaaaagaa ggggggattt
    15541 ttttggatga gagtttgtac aagcagattt taagtaatat tattatta
//
