LOCUS       sequence-307818-        8878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of long isoform of BRD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pAV5B+longBRD4
FEATURES             Location/Qualifiers
     source          1..8878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2215..2232
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4225..4359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4262..4281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4316..4335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4388..4553
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4737..5191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4824..4843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5033..5054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5217..5321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5322..5390,5391..6182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5540..5559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6353..6941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6842..6861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7095..7112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7246..7470
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7537..8070)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8259..8264,8265..8281,8282..8287))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        8639..8730
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     8655..8672
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             8813..8830
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8852..8872
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggatcctctg cggagagcgg ccctgggacg agattgagaa atctgccagt aatgggggat
       61 ggactagaaa cttcccaaat gtctacaaca caggcccagg cccaacccca gccagccaac
      121 gcagccagca ccaacccccc gcccccagag acctccaacc ctaacaagcc caagaggcag
      181 accaaccaac tgcaatacct gctcagagtg gtgctcaaga cactatggaa acaccagttt
      241 gcatggcctt tccagcagcc tgtggatgcc gtcaagctga acctccctga ttactataag
      301 atcattaaaa cgcctatgga tatgggaaca ataaagaagc gcttggaaaa caactattac
      361 tggaatgctc aggaatgtat ccaggacttc aacactatgt ttacaaattg ttacatctac
      421 aacaagcctg gagatgacat agtcttaatg gcagaagctc tggaaaagct cttcttgcaa
      481 aaaataaatg agctacccac agaagaaacc gagatcatga tagtccaggc aaaaggaaga
      541 ggacgtggga ggaaagaaac agggacagca aaacctggcg tttccacggt accaaacaca
      601 actcaagcat cgactcctcc gcagacccag acccctcagc cgaatcctcc tcctgtgcag
      661 gccacgcctc accccttccc tgccgtcacc ccggacctca tcgtccagac ccctgtcatg
      721 acagtggtgc ctccccagcc actgcagacg cccccgccag tgccccccca gccacaaccc
      781 ccacccgctc cagctcccca gcccgtacag agccacccac ccatcatcgc ggccacccca
      841 cagcctgtga agacaaagaa gggagtgaag aggaaagcag acaccaccac ccccaccacc
      901 attgacccca ttcacgagcc accctcgctg cccccggagc ccaagaccac caagctgggc
      961 cagcggcggg agagcagccg gcctgtgaaa cctccaaaga aggacgtgcc cgactctcag
     1021 cagcacccag caccagagaa gagcagcaag gtctcggagc agctcaagtg ctgcagcggc
     1081 atcctcaagg agatgtttgc caagaagcac gccgcctacg cctggccctt ctacaagcct
     1141 gtggacgtgg aggcactggg cctacacgac tactgtgaca tcatcaagca ccccatggac
     1201 atgagcacaa tcaagtctaa actggaggcc cgtgagtacc gtgatgctca ggagtttggt
     1261 gctgacgtcc gattgatgtt ctccaactgc tataagtaca accctcctga ccatgaggtg
     1321 gtggccatgg cccgcaagct ccaggatgtg ttcgaaatgc gctttgccaa gatgccggac
     1381 gagcctgagg agccagtggt ggccgtgtcc tccccggcag tgccccctcc caccaaggtt
     1441 gtggccccgc cctcatccag cgacagcagc agcgatagct cctcggacag tgacagttcg
     1501 actgatgact ctgaggagga gcgagcccag cggctggctg agctccagga gcagctcaaa
     1561 gccgtgcacg agcagcttgc agccctctct cagccccagc agaacaaacc aaagaaaaag
     1621 gagaaagaca agaaggaaaa gaaaaaagaa aagcacaaaa ggaaagagga agtggaagag
     1681 aataaaaaaa gcaaagccaa ggaacctcct cctaaaaaga cgaagaaaaa taatagcagc
     1741 aacagcaatg tgagcaagaa ggagccagcg cccatgaaga gcaagccccc tcccacgtat
     1801 gagtcggagg aagaggacaa gtgcaagcct atgtcctatg aggagaagcg gcagctcagc
     1861 ttggacatca acaagctccc cggcgagaag ctgggccgcg tggtgcacat catccagtca
     1921 cgggagccct ccctgaagaa ttccaacccc gacgagattg aaatcgactt tgagaccctg
     1981 aagccgtcca cactgcgtga gctggagcgc tatgtcacct cctgtttgcg gaagaaaagg
     2041 aaacctcaag ctgagaaagt tgatgtgatt gccggctcct ccaagatgaa gggcttctcg
     2101 tcctcagagt cggagagctc cagtgagtcc agctcctctg acagcgaaga ctccgaaaca
     2161 gagatggctc cgaagtcaaa aaagaagggg caccccggga gggagcagaa gaagcaccat
     2221 catcaccacc atcagcagat gcagcaggcc ccggctcctg tgccccagca gccgcccccg
     2281 cctccccagc agcccccacc gcctccacct ccgcagcagc aacagcagcc gccacccccg
     2341 cctcccccac cctccatgcc gcagcaggca gccccggcga tgaagtcctc gcccccaccc
     2401 ttcattgcca cccaggtgcc cgtcctggag ccccagctcc caggcagcgt ctttgacccc
     2461 atcggccact tcacccagcc catcctgcac ctgccgcagc ctgagctgcc ccctcacctg
     2521 ccccagccgc ctgagcacag cactccaccc catctcaacc agcacgcagt ggtctctcct
     2581 ccagctttgc acaacgcact accccagcag ccatcacggc ccagcaaccg agccgctgcc
     2641 ctgcctccca agcccgcccg gcccccagcc gtgtcaccag ccttgaccca aacacccctg
     2701 ctcccacagc cccccatggc ccaacccccc caagtgctgc tggaggatga agagccacct
     2761 gccccacccc tcacctccat gcagatgcag ctgtacctgc agcagctgca gaaggtgcag
     2821 ccccctacgc cgctactccc ttccgtgaag gtgcagtccc agcccccacc ccccctgccg
     2881 cccccacccc acccctctgt gcagcagcag ctgcagcagc agccgccacc acccccacca
     2941 ccccagcccc agcctccacc ccagcagcag catcagcccc ctccacggcc cgtgcacttg
     3001 cagcccatgc agttttccac ccacatccaa cagcccccgc caccccaggg ccagcagccc
     3061 ccccatccgc ccccaggcca gcagccaccc ccgccgcagc ctgccaagcc tcagcaagtc
     3121 atccagcacc accattcacc ccggcaccac aagtcggacc cctactcaac cggtcacctc
     3181 cgcgaagccc cctccccgct tatgatacat tccccccaga tgtcacagtt ccagagcctg
     3241 acccaccagt ctccacccca gcaaaacgtc cagcctaaga aacaggagct gcgtgctgcc
     3301 tccgtggtcc agccccagcc cctcgtggtg gtgaaggagg agaagatcca ctcacccatc
     3361 atccgcagcg agcccttcag cccctcgctg cggccggagc cccccaagca cccggagagc
     3421 atcaaggccc ccgtccacct gccccagcgg ccggaaatga agcctgtgga tgtcgggagg
     3481 cctgtgatcc ggcccccaga gcagaacgca ccgccaccag gggcccctga caaggacaaa
     3541 cagaaacagg agccgaagac tccagttgcg cccaaaaagg acctgaaaat caagaacatg
     3601 ggctcctggg ccagcctagt gcagaagcat ccgaccaccc cctcctccac agccaagtca
     3661 tccagcgaca gcttcgagca gttccgccgc gccgctcggg agaaagagga gcgtgagaag
     3721 gccctgaagg ctcaggccga gcacgctgag aaggagaagg agcggctgcg gcaggagcgc
     3781 atgaggagcc gagaggacga ggatgcgctg gagcaggccc ggcgggccca tgaggaggca
     3841 cgtcggcgcc aggagcagca gcagcagcag cgccaggagc aacagcagca gcagcaacag
     3901 caagcagctg cggtggctgc cgccgccacc ccacaggccc agagctccca gccccagtcc
     3961 atgctggacc agcagaggga gttggcccgg aagcgggagc aggagcgaag acgccgggaa
     4021 gccatggcag ctaccattga catgaatttc cagagtgatc tattgtcaat atttgaagaa
     4081 aatcttttct gatgaaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     4141 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     4201 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     4261 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4321 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgat atcgcctagg
     4381 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     4441 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     4501 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     4561 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     4621 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
     4681 aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatgggacgc
     4741 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4801 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4861 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4921 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4981 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa tagtggactc
     5041 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5101 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5161 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5221 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5281 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5341 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5401 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5461 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5521 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5581 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5641 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5701 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5761 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5821 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5881 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5941 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6001 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6061 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     7141 ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt aacctggcaa
     7201 aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata
     7261 aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga
     7321 atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta
     7381 tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc
     7441 gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac cgaacaactc
     7501 cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     7561 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     7621 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     7681 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag
     7741 atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag
     7801 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     7861 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     7921 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     7981 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     8041 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     8101 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     8161 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     8221 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     8281 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     8341 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     8401 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     8461 atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc
     8521 tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg
     8581 cccaggactc tagctatagt tctagtggtt ggctacgtat actccggaat attaatagat
     8641 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
     8701 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
     8761 gggcgcggat ctcggtccga actcctaaaa aaccgccacc atgtcgtact accatcacca
     8821 tcaccatcac gattacgata tcccaacgac cgaaaacctg tattttcagg gcgccatg
//
