LOCUS       sequence-307855-       10173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate hACE2 stable expressing cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10173)
  TITLE     De Francesco Lab Plasmids
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLENTI_hACE2_PURO
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2122..2425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2426..2629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2580..2600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2626..2650
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5188..5208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5193..5522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5373..5508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5532..6131
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5532..5551)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6028..6048
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6198..6786
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6251..6271)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6669..6680)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6873..7106
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7178..7299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7215..7234)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7269..7288
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7339..7474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7494..7513)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7495..7513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7523..7540)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7523..7539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7532..7554)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7681..8136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7768..7787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7978..7999
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8162..8266
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8267..8335,8336..9127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8485..8504)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9298..9886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9787..9806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10040..10057
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2161 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2221 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2281 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2341 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2401 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     2461 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2521 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2581 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     2641 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact
     2701 ctagaatgtc aagctcttcc tggctccttc tcagccttgt tgctgtaact gctgctcagt
     2761 ccaccattga ggaacaggcc aagacatttt tggacaagtt taaccacgaa gccgaagacc
     2821 tgttctatca aagttcactt gcttcttgga attataacac caatattact gaagagaatg
     2881 tccaaaacat gaataatgct ggggacaaat ggtctgcctt tttaaaggaa cagtccacac
     2941 ttgcccaaat gtatccacta caagaaattc agaatctcac agtcaagctt cagctgcagg
     3001 ctcttcagca aaatgggtct tcagtgctct cagaagacaa gagcaaacgg ttgaacacaa
     3061 ttctaaatac aatgagcacc atctacagta ctggaaaagt ttgtaaccca gataatccac
     3121 aagaatgctt attacttgaa ccaggtttga atgaaataat ggcaaacagt ttagactaca
     3181 atgagaggct ctgggcttgg gaaagctgga gatctgaggt cggcaagcag ctgaggccat
     3241 tatatgaaga gtatgtggtc ttgaaaaatg agatggcaag agcaaatcat tatgaggact
     3301 atggggatta ttggagagga gactatgaag taaatggggt agatggctat gactacagcc
     3361 gcggccagtt gattgaagat gtggaacata cctttgaaga gattaaacca ttatatgaac
     3421 atcttcatgc ctatgtgagg gcaaagttga tgaatgccta tccttcctat atcagtccaa
     3481 ttggatgcct ccctgctcat ttgcttggtg atatgtgggg tagattttgg acaaatctgt
     3541 actctttgac agttcccttt ggacagaaac caaacataga tgttactgat gcaatggtgg
     3601 accaggcctg ggatgcacag agaatattca aggaggccga gaagttcttt gtatctgttg
     3661 gtcttcctaa tatgactcaa ggattctggg aaaattccat gctaacggac ccaggaaatg
     3721 ttcagaaagc agtctgccat cccacagctt gggacctggg gaagggcgac ttcaggatcc
     3781 ttatgtgcac aaaggtgaca atggacgact tcctgacagc tcatcatgag atggggcata
     3841 tccagtatga tatggcatat gctgcacaac cttttctgct aagaaatgga gctaatgaag
     3901 gattccatga agctgttggg gaaatcatgt cactttctgc agccacacct aagcatttaa
     3961 aatccattgg tcttctgtca cccgattttc aagaagacaa tgaaacagaa ataaacttcc
     4021 tgctcaaaca agcactcacg attgttggga ctctgccatt tacttacatg ttagagaagt
     4081 ggaggtggat ggtctttaaa ggggaaattc ccaaagacca gtggatgaaa aagtggtggg
     4141 agatgaagcg agagatagtt ggggtggtgg aacctgtgcc ccatgatgaa acatactgtg
     4201 accccgcatc tctgttccat gtttctaatg attactcatt cattcgatat tacacaagga
     4261 ccctttacca attccagttt caagaagcac tttgtcaagc agctaaacat gaaggccctc
     4321 tgcacaaatg tgacatctca aactctacag aagctggaca gaaactgttc aatatgctga
     4381 ggcttggaaa atcagaaccc tggaccctag cattggaaaa tgttgtagga gcaaagaaca
     4441 tgaatgtaag gccactgctc aactactttg agcccttatt tacctggctg aaagaccaga
     4501 acaagaattc ttttgtggga tggagtaccg actggagtcc atatgcagac caaagcatca
     4561 aagtgaggat aagcctaaaa tcagctcttg gagataaagc atatgaatgg aacgacaatg
     4621 aaatgtacct gttccgatca tctgttgcat atgctatgag gcagtacttt ttaaaagtaa
     4681 aaaatcagat gattcttttt ggggaggagg atgtgcgagt ggctaatttg aaaccaagaa
     4741 tctcctttaa tttctttgtc actgcaccta aaaatgtgtc tgatatcatt cctagaactg
     4801 aagttgaaaa ggccatcagg atgtcccgga gccgtatcaa tgatgctttc cgtctgaatg
     4861 acaacagcct agagtttctg gggatacagc caacacttgg acctcctaac cagccccctg
     4921 tttccatatg gctgattgtt tttggagttg tgatgggagt gatagtggtt ggcattgtca
     4981 tcctgatctt cactgggatc agagatcgga agaagaaaaa taaagcaaga agtggagaaa
     5041 atccttatgc ctccatcgat attagcaaag gagaaaataa tccaggattc caaaacactg
     5101 atgatgttca gacctccttt ggtaccgaga cctcccaggt ggcgcccgct taaactagtt
     5161 aacgcgtcga ccagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5341 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5401 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5461 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5521 aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5581 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5641 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5701 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5761 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5821 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5881 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5941 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6001 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6061 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6121 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     6181 atgcatcgcc agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6241 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6301 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6361 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6421 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6481 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6541 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     6601 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6661 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6721 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6781 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     6841 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6901 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6961 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7021 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7081 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7141 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7201 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7261 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7321 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7381 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7441 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     7501 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7561 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7621 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     7681 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7741 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7801 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7861 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7921 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7981 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8041 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8101 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     8161 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8221 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8281 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8341 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8401 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8461 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8521 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8581 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8641 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8701 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8761 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8821 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8881 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8941 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9001 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9061 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9121 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9181 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9241 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9301 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9361 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9421 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9481 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9541 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9601 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9661 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9721 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9781 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9841 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9901 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9961 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10021 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10141 cgactggaaa gcgggcagtg agcgcaacgc aat
//
