LOCUS       sequence-307857-       11008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate hACE2 stable expressing cell line, 
            Hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Notarbartolo S, Ranzani V, Bandera A, Gruarin P, Bevilacqua V, 
            Putignano AR, Gobbini A, Galeota E, Manara C, Bombaci M, Pesce E, 
            Zagato E, Favalli A, Sarnicola ML, Curti S, Crosti M, Martinovic M, 
            Fabbris T, Marini F, Donnici L, Lorenzo M, Mancino M, Ungaro R, 
            Lombardi A, Mangioni D, Muscatello A, Aliberti S, Blasi F, De Feo T,
            Prati D, Manganaro L, Granucci F, Lanzavecchia A, De Francesco R, 
            Gori A, Grifantini R, Abrignani S
  TITLE     Integrated longitudinal immunophenotypic, transcriptional and 
            repertoire analyses delineate immune responses in COVID-19 patients.
  JOURNAL   Sci Immunol. 2021 Aug 10;6(62). pii: 6/62/eabg5021. doi: 
            10.1126/sciimmunol.abg5021.
  PUBMED    34376481
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-08-10"; volume: "6(62). pii: 6/62/eabg5021. 
            doi"; pages: " 10.1126/sciimmunol.abg5021"
COMMENT     Sequence Label: pLENTI_hACE2_HygR
FEATURES             Location/Qualifiers
     source          1..11008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2122..2425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2426..2629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2580..2600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2626..2650
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5160..5748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5213..5233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5631..5642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5804..6291
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5839..5862)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6224..6243
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6309..7334
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6309..7334
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7708..7941
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8013..8134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8050..8069)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8104..8123
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8174..8309
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8329..8348)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8330..8348)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8358..8375)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8358..8374)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8367..8389)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8516..8971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8603..8622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8813..8834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8997..9101
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9102..9170,9171..9962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9320..9339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10133..10721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10622..10641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10875..10892
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2161 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2221 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2281 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2341 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2401 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     2461 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2521 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2581 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     2641 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact
     2701 ctagaatgtc aagctcttcc tggctccttc tcagccttgt tgctgtaact gctgctcagt
     2761 ccaccattga ggaacaggcc aagacatttt tggacaagtt taaccacgaa gccgaagacc
     2821 tgttctatca aagttcactt gcttcttgga attataacac caatattact gaagagaatg
     2881 tccaaaacat gaataatgct ggggacaaat ggtctgcctt tttaaaggaa cagtccacac
     2941 ttgcccaaat gtatccacta caagaaattc agaatctcac agtcaagctt cagctgcagg
     3001 ctcttcagca aaatgggtct tcagtgctct cagaagacaa gagcaaacgg ttgaacacaa
     3061 ttctaaatac aatgagcacc atctacagta ctggaaaagt ttgtaaccca gataatccac
     3121 aagaatgctt attacttgaa ccaggtttga atgaaataat ggcaaacagt ttagactaca
     3181 atgagaggct ctgggcttgg gaaagctgga gatctgaggt cggcaagcag ctgaggccat
     3241 tatatgaaga gtatgtggtc ttgaaaaatg agatggcaag agcaaatcat tatgaggact
     3301 atggggatta ttggagagga gactatgaag taaatggggt agatggctat gactacagcc
     3361 gcggccagtt gattgaagat gtggaacata cctttgaaga gattaaacca ttatatgaac
     3421 atcttcatgc ctatgtgagg gcaaagttga tgaatgccta tccttcctat atcagtccaa
     3481 ttggatgcct ccctgctcat ttgcttggtg atatgtgggg tagattttgg acaaatctgt
     3541 actctttgac agttcccttt ggacagaaac caaacataga tgttactgat gcaatggtgg
     3601 accaggcctg ggatgcacag agaatattca aggaggccga gaagttcttt gtatctgttg
     3661 gtcttcctaa tatgactcaa ggattctggg aaaattccat gctaacggac ccaggaaatg
     3721 ttcagaaagc agtctgccat cccacagctt gggacctggg gaagggcgac ttcaggatcc
     3781 ttatgtgcac aaaggtgaca atggacgact tcctgacagc tcatcatgag atggggcata
     3841 tccagtatga tatggcatat gctgcacaac cttttctgct aagaaatgga gctaatgaag
     3901 gattccatga agctgttggg gaaatcatgt cactttctgc agccacacct aagcatttaa
     3961 aatccattgg tcttctgtca cccgattttc aagaagacaa tgaaacagaa ataaacttcc
     4021 tgctcaaaca agcactcacg attgttggga ctctgccatt tacttacatg ttagagaagt
     4081 ggaggtggat ggtctttaaa ggggaaattc ccaaagacca gtggatgaaa aagtggtggg
     4141 agatgaagcg agagatagtt ggggtggtgg aacctgtgcc ccatgatgaa acatactgtg
     4201 accccgcatc tctgttccat gtttctaatg attactcatt cattcgatat tacacaagga
     4261 ccctttacca attccagttt caagaagcac tttgtcaagc agctaaacat gaaggccctc
     4321 tgcacaaatg tgacatctca aactctacag aagctggaca gaaactgttc aatatgctga
     4381 ggcttggaaa atcagaaccc tggaccctag cattggaaaa tgttgtagga gcaaagaaca
     4441 tgaatgtaag gccactgctc aactactttg agcccttatt tacctggctg aaagaccaga
     4501 acaagaattc ttttgtggga tggagtaccg actggagtcc atatgcagac caaagcatca
     4561 aagtgaggat aagcctaaaa tcagctcttg gagataaagc atatgaatgg aacgacaatg
     4621 aaatgtacct gttccgatca tctgttgcat atgctatgag gcagtacttt ttaaaagtaa
     4681 aaaatcagat gattcttttt ggggaggagg atgtgcgagt ggctaatttg aaaccaagaa
     4741 tctcctttaa tttctttgtc actgcaccta aaaatgtgtc tgatatcatt cctagaactg
     4801 aagttgaaaa ggccatcagg atgtcccgga gccgtatcaa tgatgctttc cgtctgaatg
     4861 acaacagcct agagtttctg gggatacagc caacacttgg acctcctaac cagccccctg
     4921 tttccatatg gctgattgtt tttggagttg tgatgggagt gatagtggtt ggcattgtca
     4981 tcctgatctt cactgggatc agagatcgga agaagaaaaa taaagcaaga agtggagaaa
     5041 atccttatgc ctccatcgat attagcaaag gagaaaataa tccaggattc caaaacactg
     5101 atgatgttca gacctccttt ggtaccgaga cctcccaggt ggcgcccgct taagtcgaca
     5161 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5221 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5281 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5341 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5401 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5461 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5521 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5581 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5641 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5701 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctgc
     5761 agatatccgg actagtgatc tctcgaggtt aacgaattct accgggtagg ggaggcgctt
     5821 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     5881 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     5941 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     6001 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     6061 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     6121 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     6181 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     6241 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcatc
     6301 ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     6361 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     6421 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     6481 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     6541 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     6601 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     6661 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     6721 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     6781 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     6841 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     6901 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     6961 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     7021 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     7081 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     7141 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     7201 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     7261 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     7321 gggcaaagga atagagtaga tgccgaccga acaagagctg atttcgagaa cgcctcagcc
     7381 agcaactcgc gcgagcctag caaggcaaat gcgagagaac ggccttacgc ttggtggcac
     7441 agttctcgtc cacagttcgc taagctcgct cggctgggtc gcgggagggc cggtcgcagt
     7501 gattcaggcc cttctggatt gtgttggtcc ccagggcacg attgtcatgc ccacgcactc
     7561 gggtgatctg actgatcccg cagattggag atcgccgccc gtgcctgccg attgggtgca
     7621 gatccgtcga gggcccggta cctttaagac caatgactta caaggcagct gtagatctta
     7681 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
     7741 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7801 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7861 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7921 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7981 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8041 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8101 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8161 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8221 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8281 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8341 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8401 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8461 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8521 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8581 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8641 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8701 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8761 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8821 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8881 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8941 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     9001 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9061 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9121 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9181 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9241 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9301 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9361 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9421 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9481 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9541 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9601 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9661 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9721 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9781 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9841 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9901 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9961 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10021 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10261 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10321 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10381 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10441 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10501 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10561 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10621 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10681 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10741 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10801 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10861 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10921 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10981 ggaaagcggg cagtgagcgc aacgcaat
//
