LOCUS       sequence-307858-        9118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of spike with C-term deletion of 19 aa, used to generate 
            high efficiency SARS-CoV-2-pseudotyped lentiviral particles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Conforti A, Marra E, Palombo F, Roscilli G, Rava M, Fumagalli V, 
            Muzi A, Maffei M, Luberto L, Lione L, Salvatori E, Compagnone M, 
            Pinto E, Pavoni E, Bucci F, Vitagliano G, Stoppoloni D, Pacello ML, 
            Cappelletti M, Ferrara FF, D'Acunto E, Chiarini V, Arriga R, Nyska 
            A, Di Lucia P, Marotta D, Bono E, Giustini L, Sala E, Perucchini C, 
            Paterson J, Ryan KA, Challis AR, Matusali G, Colavita F, Caselli G, 
            Criscuolo E, Clementi N, Mancini N, Gross R, Seidel A, Wettstein L, 
            Munch J, Donnici L, Conti M, De Francesco R, Kuka M, Ciliberto G, 
            Castilletti C, Capobianchi MR, Ippolito G, Guidotti LG, Rovati L, 
            Iannacone M, Aurisicchio L
  TITLE     COVID-eVax, an electroporated DNA vaccine candidate encoding the 
            SARS-CoV-2 RBD, elicits protective responses in animal models.
  JOURNAL   Mol Ther. 2021 Sep 20. pii: S1525-0016(21)00466-4. doi: 
            10.1016/j.ymthe.2021.09.011.
  PUBMED    34547465
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2021 Sep 20. pii: S1525-0016(21)00466-4. doi: 
            10.1016/j.ymthe.2021.09.011."
COMMENT     Sequence Label: pcDNA3.1_ spike_del19
FEATURES             Location/Qualifiers
     source          1..9118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4475..4492)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4481..4705
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4751..5179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4838..4857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5048..5069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5188..5208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5193..5522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5373..5508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5435..5454
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5589..6383
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5643..5662)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6253..6272
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6557..6678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6594..6613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6648..6667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6727..6743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6727..6743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6740..6762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6751..6767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6775..6781,6782..6799,6800..6805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6820..6841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6958..6975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7129..7717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7209..7228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7888..8679,8680..8748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8511..8530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8749..8853)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8928..8947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcatgt ttgtcttcct ggtcctgctg cctctggtct cgtctcagtg cgtgaacctg
      721 actactagaa cccagctgcc tcctgcctat actaactcct tcacccgcgg cgtgtactac
      781 ccagacaagg tgttccgcag ctccgtgctg cactccaccc aggatctgtt cctgcccttc
      841 ttcagcaacg tgacctggtt ccacgccatc cacgtgagcg gcaccaatgg caccaagcgg
      901 ttcgacaatc ccgtgctgcc attcaacgat ggcgtgtact tcgcctccac cgagaagagc
      961 aacatcatcc gcggctggat cttcggcacc accctggact ccaagaccca gagcctgctg
     1021 atcgtgaaca atgccaccaa cgtggtcatc aaggtgtgcg agttccagtt ctgcaatgat
     1081 ccattcctgg gcgtgtacta ccacaagaac aataagtcct ggatggagag cgagttccgc
     1141 gtgtacagct ccgccaacaa ttgcaccttc gagtacgtgt cccagccctt cctgatggac
     1201 ctggagggca agcagggcaa tttcaagaac ctgcgcgagt tcgtgttcaa gaatatcgat
     1261 ggctacttca agatctactc caagcacacc cccatcaacc tggtgcgcga cctgccacag
     1321 ggcttcagcg ccctggagcc actggtggat ctgccaatcg gcatcaacat caccaggttc
     1381 cagaccctgc tggccctgca ccgcagctac ctgaccccag gcgacagctc cagcggatgg
     1441 accgctggag ctgctgccta ctacgtgggc tacctgcagc cccgcacctt cctgctgaag
     1501 tacaacgaga atggcaccat caccgacgcc gtggattgcg ccctggatcc actgtccgag
     1561 acaaagtgca ccctgaagag cttcaccgtg gagaagggca tctaccagac ctccaatttc
     1621 cgcgtgcagc caaccgagag catcgtgcgc ttccccaata tcaccaacct gtgcccattc
     1681 ggcgaggtgt tcaacgctac caggttcgcc agcgtgtacg cttggaatcg caagcgcatc
     1741 tccaactgcg tggccgacta cagcgtgctg tacaactccg ccagcttctc caccttcaag
     1801 tgctacggcg tgtcccccac caagctgaat gatctgtgct tcaccaacgt gtacgccgat
     1861 agcttcgtga tcaggggcga cgaggtgcgc cagatcgctc caggacagac cggcaagatc
     1921 gctgactaca attacaagct gcccgacgat ttcaccggct gcgtgatcgc ctggaactcc
     1981 aacaatctgg atagcaaagt gggcggcaac tacaattacc tgtaccgcct gttccgcaag
     2041 tccaatctga agccattcga gcgcgacatc tccaccgaga tctaccaggc tggaagcacc
     2101 ccatgcaatg gagtggaggg cttcaactgc tacttccccc tgcagagcta cggcttccag
     2161 ccaaccaacg gagtgggata ccagccatac agggtggtgg tgctgtcctt cgagctgctg
     2221 cacgctccag ctaccgtgtg cggaccaaag aagagcacca atctggtgaa gaacaagtgc
     2281 gtgaacttca atttcaacgg cctgaccgga accggcgtgc tgaccgagtc caacaagaag
     2341 ttcctgccat tccagcagtt cggaagggac atcgctgata ccaccgacgc cgtgcgcgac
     2401 ccacagaccc tggagatcct ggatatcacc ccatgctcct tcggcggcgt gagcgtgatc
     2461 accccaggaa ccaataccag caaccaggtg gccgtgctgt accaggacgt gaattgcacc
     2521 gaggtgccag tggctatcca cgctgatcag ctgaccccaa cctggcgcgt gtacagcacc
     2581 ggatccaacg tgttccagac ccgcgccgga tgcctgatcg gagctgagca cgtgaacaat
     2641 tcctacgagt gcgacatccc aatcggagct ggaatctgcg ccagctacca gacccagacc
     2701 aactccccaa ggagggctcg cagcgtggcc agccagtcca tcatcgccta caccatgtcc
     2761 ctgggcgccg agaatagcgt ggcctacagc aacaattcca tcgccatccc aaccaacttc
     2821 accatctccg tgaccaccga gatcctgccc gtgtccatga ccaagaccag cgtggactgc
     2881 accatgtaca tctgcggcga ttccaccgag tgcagcaacc tgctgctgca gtacggcagc
     2941 ttctgcaccc agctgaatcg cgccctgacc ggaatcgctg tggagcagga taagaacacc
     3001 caggaggtgt tcgcccaggt gaagcagatc tacaagaccc ccccaatcaa ggacttcggc
     3061 ggcttcaatt tcagccagat cctgcccgat ccaagcaagc cctccaagcg cagcttcatc
     3121 gaggacctgc tgttcaacaa ggtgaccctg gccgatgccg gcttcatcaa gcagtacggc
     3181 gattgcctgg gcgacatcgc tgcccgcgac ctgatctgcg cccagaagtt caatggcctg
     3241 accgtgctgc caccactgct gaccgatgag atgatcgctc agtacacctc cgccctgctg
     3301 gccggaacca tcaccagcgg atggaccttc ggcgctggag ccgccctgca gatccccttc
     3361 gccatgcaga tggcctaccg cttcaacggc atcggcgtga cccagaatgt gctgtacgag
     3421 aaccagaagc tgatcgccaa tcagttcaac tccgccatcg gcaagatcca ggactccctg
     3481 tccagcaccg ccagcgccct gggcaagctg caggatgtgg tgaatcagaa cgcccaggcc
     3541 ctgaataccc tggtgaagca gctgtccagc aacttcggcg ccatctccag cgtgctgaat
     3601 gatatcctga gccgcctgga caaggtggag gctgaggtgc agatcgatag gctgatcacc
     3661 ggccgcctgc agtccctgca gacctacgtg acccagcagc tgatcagggc tgctgagatc
     3721 agggccagcg ccaatctggc tgctaccaag atgtccgagt gcgtgctggg acagagcaag
     3781 agggtggact tctgcggcaa gggctaccac ctgatgtcct tcccacagag cgccccacac
     3841 ggagtggtgt tcctgcacgt gacctacgtg ccagcccagg agaagaactt caccaccgct
     3901 ccagctatct gccacgatgg caaggctcac ttcccacgcg agggcgtgtt cgtgtccaac
     3961 ggcacccact ggttcgtgac ccagcgcaat ttctacgagc cccagatcat caccaccgac
     4021 aataccttcg tgagcggcaa ctgcgacgtg gtcatcggaa tcgtgaacaa taccgtgtac
     4081 gatcccctgc agccagagct ggactccttc aaggaggagc tggataagta cttcaagaat
     4141 cacaccagcc ccgacgtgga tctgggcgac atctccggca tcaatgccag cgtggtgaac
     4201 atccagaagg agatcgaccg cctgaacgag gtggccaaga atctgaacga gtccctgatc
     4261 gatctgcagg agctgggcaa gtacgagcag tacatcaagt ggccatggta catctggctg
     4321 ggcttcatcg ccggcctgat cgccatcgtg atggtgacca tcatgctgtg ctgcatgacc
     4381 tcctgctgca gctgcctgaa gggctgctgc tcctgcggca gctgctgcta ggaattcctc
     4441 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4501 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4561 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4621 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4681 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4741 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4801 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4861 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4921 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4981 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5041 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5101 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5161 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5341 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5401 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5461 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5521 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5581 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5641 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5701 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5761 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5821 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5881 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5941 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6001 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6061 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6121 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6181 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6241 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6301 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6361 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6421 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6481 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6541 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6601 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6661 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6721 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6781 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6841 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6901 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6961 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7021 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7081 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7141 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7201 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7261 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7321 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7381 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7441 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7501 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7561 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7621 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7681 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7741 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7801 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7861 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7921 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7981 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8041 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8101 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8161 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8221 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8281 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8341 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8401 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8461 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8521 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8581 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8641 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8701 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8761 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8821 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8881 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     8941 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     9001 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     9061 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
