LOCUS       sequence-307890-        5296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfYFP tagged with ssrA from the PlLacO1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5296)
  TITLE     Bennett Lab Plasmids and Strains
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDZ115
FEATURES             Location/Qualifiers
     source          1..5296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(646..673)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    1412..1428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1417..1439
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1630..1659
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1844..1860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1945..2658
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2855..2926
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             3136..3327
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3321..3343)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3429..3568
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3484..3503
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3583..3600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3754..4342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3834..3853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4453..4472)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4464..5279
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4464..5279
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4537..4556)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
       61 ggctagccgc ctggtgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
      121 cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag ccgcttatgt
      181 ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt gcttctcaaa
      241 tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg atatgatcat
      301 ttattctgcc tcccagagcc tgataaaaac ggttagcgct tcgttaatac agatgtaggt
      361 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
      421 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgctgcc
      481 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc
      541 atgccccgcg cccaccggaa ggagctaccg cctgcggccg ctaggaaata ataaaaaagc
      601 cggattaata atctggcttt ttatattctc tctctagtat ataaacgcag aaaggcccac
      661 ccgaaggtga gccagtgtga gacagcggtg cggactgttg taactcagaa taagaaatga
      721 ggccgctcat ggcgttgact ctcagtcata gtatcgtggt atcaccggtt ggttccactc
      781 tctgttgcgg gcaacttcag cagcacgtag gggaagcttt tattactcga gaagcgcgtc
      841 tgaataggct tcttgtattc ctgccacaca gggaaaccat caggagttac ccaatgcaca
      901 gcgcaacgct tgcgaagaat ctctccagtc ttcttatctt tgacctcagc agccagcagc
      961 ttagcagcag acttaagcca gttcattgct tcaaccgcag ctaccaccgt cacgctcaca
     1021 gattcccaaa tcagcttagc catgtatcca gcagcctgat tcggctgagt gaacatcaga
     1081 cccttgccgg aatcaatagc tggctgaatg gtatcttcca gcacttgttg acggaagccg
     1141 aactctttgg acccgtaagc cagcgtcatg actgaacgct tagtcacact gcgagtaaca
     1201 ccgtaagcca gccattgacc agccagtgcc ttagtgccca gcttgacttt ctcagagatt
     1261 tcaccagtgt tctcatcggt cacggtaact acttcgttat cggtcccatt gattgcgtct
     1321 gcttgtagaa tctcgttgac tttcttagca acaatcccgt agatgtcctg aacggtttca
     1381 ctaggaagca agttaagacg tctgtgtgga attgtgagcg gataacaatt tcacacaggg
     1441 ccctcggaca ccgaggagaa tgtcaagagg cgaacacaca acgtcttgga gcgccagagg
     1501 aggaacgagc taaaacggag cttttttgcc ctgcgtgacc agatcccgga gttggaaaac
     1561 aatgaaaagg cccccaaggt agttatcctt aaaaaagcca cagcatacat cctgtccgtc
     1621 caagcagagg agcaaaagct catttctgaa gaggacttgt tgcggaaacg acgagaacag
     1681 ttgaaacaca aacttgaaca gctacggaac tcttgtgcgt aaggaaaagt aaggaaaacg
     1741 attccttcta acagaaatgt cctgagcaat cacctatgaa ctgtcgaggg ggcctatctg
     1801 agggaattta cttgtgagcg ctcacaattg atactgagca caattgtgag cggataacaa
     1861 ttgcatgcca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta
     1921 atcatgctaa ggagaaatac tagtatgcgt aaaggcgaag agctgttcac tggtgtcgtc
     1981 cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag
     2041 ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa
     2101 ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct
     2161 cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat
     2221 gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg
     2281 aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa
     2341 gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc
     2401 accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag
     2461 gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct
     2521 gttctgctgc cagacaatca ctatctgagc taccaaagcg ttctgtctaa agatccgaac
     2581 gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt
     2641 atggatgaac tgtacaaagc agcgaacgac gaaaattacg cccttgcagc gtaataagag
     2701 ctcgaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     2761 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     2821 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     2881 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcataa
     2941 gtgcggaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     3001 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     3061 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
     3121 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
     3181 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
     3241 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
     3301 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3361 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3421 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     3481 atgcggcatc agagcagatt gtactgagag tgcaccattg cggtgtgaaa taccgcacag
     3541 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     3601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgcttaca
     4441 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctctaggc
     4501 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     4561 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     4621 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     4681 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     4741 atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat
     4801 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     4861 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     4921 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     4981 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     5041 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     5101 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     5161 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     5221 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag
     5281 aattaattca tgagcg
//
