LOCUS       sequence-307891-        5277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sfYFP tagged with ssrA from the Pcin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5277)
  TITLE     Bennett Lab Plasmids and Strains
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDZ114
FEATURES             Location/Qualifiers
     source          1..5277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(646..673)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    1412..1428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1417..1439
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1630..1659
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1794..1810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1825..1841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1926..2639
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2836..2907
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             3117..3308
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3302..3324)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3410..3549
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3465..3484
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3564..3581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3735..4323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3815..3834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4434..4453)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4445..5260
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4445..5260
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4518..4537)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
       61 ggctagccgc ctggtgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
      121 cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag ccgcttatgt
      181 ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt gcttctcaaa
      241 tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg atatgatcat
      301 ttattctgcc tcccagagcc tgataaaaac ggttagcgct tcgttaatac agatgtaggt
      361 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
      421 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgctgcc
      481 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc
      541 atgccccgcg cccaccggaa ggagctaccg cctgcggccg ctaggaaata ataaaaaagc
      601 cggattaata atctggcttt ttatattctc tctctagtat ataaacgcag aaaggcccac
      661 ccgaaggtga gccagtgtga gacagcggtg cggactgttg taactcagaa taagaaatga
      721 ggccgctcat ggcgttgact ctcagtcata gtatcgtggt atcaccggtt ggttccactc
      781 tctgttgcgg gcaacttcag cagcacgtag gggaagcttt tattactcga gaagcgcgtc
      841 tgaataggct tcttgtattc ctgccacaca gggaaaccat caggagttac ccaatgcaca
      901 gcgcaacgct tgcgaagaat ctctccagtc ttcttatctt tgacctcagc agccagcagc
      961 ttagcagcag acttaagcca gttcattgct tcaaccgcag ctaccaccgt cacgctcaca
     1021 gattcccaaa tcagcttagc catgtatcca gcagcctgat tcggctgagt gaacatcaga
     1081 cccttgccgg aatcaatagc tggctgaatg gtatcttcca gcacttgttg acggaagccg
     1141 aactctttgg acccgtaagc cagcgtcatg actgaacgct tagtcacact gcgagtaaca
     1201 ccgtaagcca gccattgacc agccagtgcc ttagtgccca gcttgacttt ctcagagatt
     1261 tcaccagtgt tctcatcggt cacggtaact acttcgttat cggtcccatt gattgcgtct
     1321 gcttgtagaa tctcgttgac tttcttagca acaatcccgt agatgtcctg aacggtttca
     1381 ctaggaagca agttaagacg tctgtgtgga attgtgagcg gataacaatt tcacacaggg
     1441 ccctcggaca ccgaggagaa tgtcaagagg cgaacacaca acgtcttgga gcgccagagg
     1501 aggaacgagc taaaacggag cttttttgcc ctgcgtgacc agatcccgga gttggaaaac
     1561 aatgaaaagg cccccaaggt agttatcctt aaaaaagcca cagcatacat cctgtccgtc
     1621 caagcagagg agcaaaagct catttctgaa gaggacttgt tgcggaaacg acgagaacag
     1681 ttgaaacaca aacttgaaca gctacggaac tcttgtgcgt aaggaaaagt aaggaaaacg
     1741 attccttcta acagaaatgt cctgagcaat cacctatgaa ctgtcgattg acattgtgag
     1801 cggataacaa gatactgagc acaattgtga gcggataaca attgcatgcc acttaaaaag
     1861 gagatcaaca atgaaagcaa ttttcgtact gaaacatctt aatcatgcta aggagaaata
     1921 ctagtatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg
     1981 atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta
     2041 atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga
     2101 ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga
     2161 agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt
     2221 cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc
     2281 tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc
     2341 ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa
     2401 atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg
     2461 ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc
     2521 actatctgag ctaccaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg
     2581 ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaag
     2641 cagcgaacga cgaaaattac gcccttgcag cgtaataaga gctcgaacgc agaagcggtc
     2701 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     2761 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     2821 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2881 atctgttgtt tgtcggtgaa cgctctctac tagagtcata agtgcggaag cgctggcatt
     2941 gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac
     3001 aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc
     3061 gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga
     3121 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
     3181 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
     3241 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
     3301 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     3361 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     3421 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     3481 tgtactgaga gtgcaccatt gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     3541 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4381 taagggattt tggtcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     4441 tgttatgagc catattcaac gggaaacgtc ttgctctagg ccgcgattaa attccaacat
     4501 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     4561 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     4621 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     4681 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     4741 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     4801 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     4861 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     4921 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     4981 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     5041 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     5101 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     5161 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     5221 taaattgcag tttcatttga tgctcgatga gtttttctaa gaattaattc atgagcg
//
