LOCUS       sequence-307895-        6357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For recombination of HDLBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni
            G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, 
            Kempa S, Huttelmaier S, Selbach M, Landthaler M
  TITLE     HDLBP binds ER-targeted mRNAs by multivalent interactions to promote
            protein synthesis of transmembrane and secreted proteins.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7.
  PUBMED    35585045
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2727. doi"; pages: " 
            10.1038/s41467-022-30322-7"
COMMENT     Sequence Label: pDONR221_HDLBP
FEATURES             Location/Qualifiers
     source          1..6357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..28)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(120..206)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     255..277
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     269..286
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     270..286
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    302..401
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4215..4314)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4331..4350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4332..4350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4355..4371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4355..4371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4473..4492)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4484..5293
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4551..4570)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5440..6028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5929..5948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6182..6199
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
       61 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
      121 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
      181 tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct
      241 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc
      301 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
      361 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tccaccatga gttccgttgc
      421 agttttgacc caagagagtt ttgctgaaca ccgaagtggg ctggttccgc aacaaatcaa
      481 agttgccact ctaaattcag aagaggagag cgaccctcca acctacaagg atgccttccc
      541 tccacttcct gagaaagctg cttgcctgga aagtgcccag gaacccgctg gagcctgggg
      601 gaacaagatc cgacccatca aggcttctgt catcactcag gtgttccatg tacccctgga
      661 ggagagaaaa tacaaggata tgaaccagtt tggagaaggt gaacaagcaa aaatctgcct
      721 tgagatcatg cagagaactg gtgctcactt ggagctgtct ttggccaaag accaaggcct
      781 ctccatcatg gtgtcaggaa agctggatgc tgtcatgaaa gctcggaagg acattgttgc
      841 tagactgcag actcaggcct cagcaactgt tgccattccc aaagaacacc atcgctttgt
      901 tattggcaaa aatggagaga aactgcaaga cttggagcta aaaactgcaa ccaaaatcca
      961 gatcccacgc ccagatgacc ccagcaatca gatcaagatc actggcacca aagagggcat
     1021 cgagaaagct cgccatgaag tcttactcat ctctgccgag caggacaaac gtgctgtgga
     1081 gaggctagaa gtagaaaagg cattccaccc cttcatcgct gggccgtata atagactggt
     1141 tggcgagatc atgcaggaga caggcacgcg catcaacatc cccccaccca gcgtgaaccg
     1201 gacagagatt gtcttcactg gagagaagga acagttggct caggctgtgg ctcgcatcaa
     1261 gaagatttat gaggagaaga aaaagaagac tacaaccatt gcagtggaag tgaagaaatc
     1321 ccaacacaag tatgtcattg ggcccaaggg caattcattg caggagatcc ttgagagaac
     1381 tggagtttcc gttgagatcc caccctcaga cagcatctct gagactgtaa tacttcgagg
     1441 cgaacctgaa aagttaggtc aggcgttgac tgaagtctat gccaaggcca atagcttcac
     1501 cgtctcctct gtcgccgccc cttcctggct tcaccgtttc atcattggca agaaagggca
     1561 gaacctggcc aaaatcactc agcagatgcc aaaggttcac atcgagttca cagagggcga
     1621 agacaagatc accctggagg gccctacaga ggatgtcaat gtggcccagg aacagataga
     1681 aggcatggtc aaagatttga ttaaccggat ggactatgtg gagatcaaca tcgaccacaa
     1741 gttccacagg cacctcattg ggaagagcgg tgccaacata aacagaatca aagaccagta
     1801 caaggtgtcc gtgcgcatcc ctcctgacag tgagaagagc aatttgatcc gcatcgaggg
     1861 ggacccacag ggcgtgcagc aggccaagcg agagctgctg gagcttgcat ctcgcatgga
     1921 aaatgagcgt accaaggatc taatcattga gcaaagattt catcgcacaa tcattgggca
     1981 gaagggtgaa cggatccgtg aaattcgtga caaattccca gaggtcatca ttaactttcc
     2041 agacccagca caaaaaagtg acattgtcca gctcagagga cctaagaatg aggtggaaaa
     2101 atgcacaaaa tacatgcaga agatggtggc agatctggtg gaaaatagct attcaatttc
     2161 tgttccgatc ttcaaacagt ttcacaagaa tatcattggg aaaggaggcg caaacattaa
     2221 aaagattcgt gaagaaagca acaccaaaat cgaccttcca gcagagaata gcaattcaga
     2281 gaccattatc atcacaggca agcgagccaa ctgcgaagct gcccggagca ggattctgtc
     2341 tattcagaaa gacctggcca acatagccga ggtagaggtc tccatccctg ccaagctgca
     2401 caactccctc attggcacca agggccgtct gatccgctcc atcatggagg agtgcggcgg
     2461 ggtccacatt cactttcccg tggaaggttc aggaagcgac accgttgtta tcaggggccc
     2521 ttcctcggat gtggagaagg ccaagaagca gctcctgcat ctggcggagg agaagcaaac
     2581 caagagtttc actgttgaca tccgcgccaa gccagaatac cacaaattcc tcatcggcaa
     2641 ggggggcggc aaaattcgca aggtgcgcga cagcactgga gcacgtgtca tcttccctgc
     2701 ggctgaggac aaggaccagg acctgatcac catcattgga aaggaggacg ccgtccgaga
     2761 ggcacagaag gagctggagg ccttgatcca aaacctggat aatgtggtgg aagactccat
     2821 gctggtggac cccaagcacc accgccactt cgtcatccgc agaggccagg tcttgcggga
     2881 gattgctgaa gagtatggcg gggtgatggt cagcttccca cgctctggca cacagagcga
     2941 caaagtcacc ctcaagggcg ccaaggactg tgtggaggca gccaagaaac gcattcagga
     3001 gatcattgag gacctggaag ctcaggtgac attagaatgt gctatacccc agaaattcca
     3061 tcgatctgtc atgggcccca aaggttccag aatccagcag attactcggg atttcagtgt
     3121 tcaaattaaa ttcccagaca gagaggagaa cgcagttcac agtacagagc cagttgtcca
     3181 ggagaatggg gacgaagctg gggaggggag agaggctaaa gattgtgacc ccggctctcc
     3241 aaggaggtgt gacatcatca tcatctctgg ccggaaagaa aagtgtgagg ctgccaagga
     3301 agctctggag gcattggttc ctgtcaccat tgaagtagag gtgccctttg accttcaccg
     3361 ttacgttatt gggcagaaag gaagtgggat ccgcaagatg atggatgagt ttgaggtgaa
     3421 catacatgtc ccggcacctg agctgcagtc tgacatcatc gccatcacgg gcctcgctgc
     3481 aaatttggac cgggccaagg ctggactgct ggagcgtgtg aaggagctac aggccgagca
     3541 ggaggaccgg gctttaagga gttttaagct gagtgtcact gtagacccca aataccatcc
     3601 caagattatc gggagaaagg gggcagtaat tacccaaatc cggttggagc atgacgtgaa
     3661 catccagttt cctgataagg acgatgggaa ccagccccag gaccaaatta ccatcacagg
     3721 gtacgaaaag aacacagaag ctgccaggga tgctatactg agaattgtgg gtgaacttga
     3781 gcagatggtt tctgaggacg tcccgctgga ccaccgcgtt cacgcccgca tcattggtgc
     3841 ccgcggcaaa gccattcgca aaatcatgga cgaattcaag gtggacattc gcttcccaca
     3901 gagcggagcc ccagacccca actgcgtcac tgtgacgggg ctcccagaga atgtggagga
     3961 agccatcgac cacatcctca atctggagga ggaataccta gctgacgtgg tggacagtga
     4021 ggcgctgcag gtatacatga aacccccagc acacgaagag gccaaggcac cttccagagg
     4081 ctttgtggtg cgggacgcac cctggaccgc cagcagcagt gagaaggctc ctgacatgag
     4141 cagctctgag gaatttccca gctttggggc tcaggtggct cccaagaccc tcccttgggg
     4201 ccccaaacga taggacccag ctttcttgta caaagttggc attataagaa agcattgctt
     4261 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     4321 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     4381 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     4441 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     4501 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     4561 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     4621 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     4681 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     4741 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     4801 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     4861 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     4921 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     4981 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     5041 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     5101 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     5161 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     5221 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     5281 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     5341 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     5401 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5461 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5521 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5581 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5641 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5701 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5761 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5821 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5881 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5941 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6001 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6061 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6121 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6181 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6241 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6301 tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgca
//
