LOCUS       sequence-307904-       11956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insertion of split hygromycin landing pad into ChrII:8420157 site C.
            elegans strains via CRISPR with SEC selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Stevenson ZC, Moerdyk-Schauwecker MJ, Jamison B, Phillips PC
  TITLE     Rapid Self-Selecting and Clone-Free Integration of Transgenes into 
            Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans.
  JOURNAL   G3 (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400.
  PUBMED    32816924
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400."
COMMENT     Sequence Label: pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)
FEATURES             Location/Qualifiers
     source          1..11956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(157..745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(237..256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(916..1707,1708..1776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1539..1558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1777..1881)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1949..1967
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2005..2027)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    complement(5003..5036)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5046..5075
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(6892..7215)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    9133..9157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9160..10185
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9160..10185
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(10186..10210)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10563..10596)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgacagggcc ctgtgaaatt gttatccgct ggtatcagct cactcaaagg cggtaatacg
       61 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      121 gaccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
      901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
      961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1021 cttaccatct gaccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     1921 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     1981 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2041 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2101 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgcactga gagtgcacca
     2161 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     2221 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2281 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2341 cccagtcacg acgtcacagc acgtgtgttt ttgaacacgg cgatatgtat ctgtagatca
     2401 tccccaattt tcaacaatga atctaaatac tgaacttttt gtgtaatctt tagtgcagaa
     2461 aaatgaccaa aatcagcgat tttccgatta tacccaaaat atctcagctc aatgagcacc
     2521 tataaaaatg taaaaataaa gaaagtagtc gaaccatatt ttgtaaaggc taaaaaactg
     2581 aataaatttt tatccatcca gcgcggcgtt acaatccgtg gcccatgtca cgcacggctt
     2641 tcaaagaaaa aaaaacagtc tgtttgcgcg acatcgatct ggcaaaaatc ctaaaaacga
     2701 ctatgcgtgg ctcactttat attaatatga aaaatacaaa cttggtggtg caggtgctgc
     2761 agaggccatc gatgaagaag tggaggcctt gttgtcgatc tacaaaattg tatggttgtt
     2821 tgagattttc cttgaaacgc tctcaatctt ggatatgata taaattctgg ataagatatg
     2881 atggatatga tctatgtttg atagagtgca tttgaaagga taacggccct gcagacattt
     2941 ttgaaaaaat aacaaacatt taattttctc actcgtttag gctattcccc tatttttgat
     3001 attccttcgc acatatgaaa actacttttt ttcgaaactg ttaactccag aatttataaa
     3061 tctatagccc ttacttgatt atttattatc atggttactc acctcatgtc tctttctctt
     3121 ttcaagaagt cttcgttcat atctgttgtc tctttcctcc ttttgaatct ctgctttgct
     3181 cttcgccatt gtttcctgaa aataatgtaa cttgaattgt gtaatatact tttttaattt
     3241 gaatttggct tgtaacgcgg aatcactacg tgcgggatca tttcttacta gaaaacccag
     3301 aaaatgccat atttcactta tctcggggtc atttctaatt agaaaagcta caaaaaccat
     3361 ttctaattac ggtgacttta gaactggtcg aaatagctaa ttagaaatgg gtttttgcta
     3421 attagaggtg actctatgtt atctgagtaa gcctcgtttg tatgtaaact cacagtacta
     3481 ataaatgcaa gacacccggg tttgtctaga tatgaaataa ttgaaatatc aattctgaca
     3541 gacaataatg gtaatcttga taaggagttc cacgcccagg agaacacgtt agttttcttg
     3601 tttttgattg cgtgcgttat tttggagaaa aactcgattt tttacaaaat aattttttga
     3661 aaggaacact gttcaataag ttttgtcttt tttctcagtt gtgatacggt tttttattct
     3721 tttttgtagt tatacagaag accgcctgca ggggacagtc ctgccgaggt ggagggaccg
     3781 ttacgtctac cgtcacttcg cctccgccgc cctcccaatc ccagaggtcc tcgacatcgg
     3841 agagttctcc gagtccctca cctactgcat ctcccgtcgt gcccaaggag tcaccctcca
     3901 agacctccca gagaccgagc tcccagccgt cctccaacca gtcgccgagg ccatggacgc
     3961 catcgccgcc gccgacctct cccaaacctc cggattcgga ccattcggac cacaaggaat
     4021 cggacaatac accacctggc gtgacttcat ctgcgccatc gccgacccac acgtctacca
     4081 ctggcaaacc gtcatggacg acaccgtctc cgcctccgtc gcccaagccc tcgacgagct
     4141 catgctctgg gccgaggact gcccagaggt ccgtcacctc gtccacgccg acttcggatc
     4201 caacaacgtc ctcaccgaca acggacgtat caccgccgtc atcgactggt ccgaggccat
     4261 gttcggagac tcccaatacg aggtcgccaa catcttcttc tggcgtccat ggctcgcctg
     4321 catggagcaa caaacccgtt acttcgagcg tcgtcaccca gagctcgccg gatccccacg
     4381 tctccgtgcc tacatgctcc gtatcggact cgaccaactc taccaatccc tcgtcgacgg
     4441 aaacttcgac gacgccgcct gggcccaagg acgttgcgac gccatcgtcc gttccggagc
     4501 cggaaccgtc ggacgtaccc aaatcgcccg tcgttccgcc gccgtctgga ccgacggatg
     4561 cgtcgaggtc ctcgccgact ccggaaaccg tcgtccatcc acccgtccac gtgccaagga
     4621 gtaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct
     4681 atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc
     4741 acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga
     4801 aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt
     4861 ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa
     4921 accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttacta
     4981 gtgcaggagc tgtaagttta aaataacttc gtatagcata cattatacga agttattttc
     5041 agggagagca aaagcttatc tccgaggagg acctctaagg atgatcgacg ccaacgtcgt
     5101 tgaattttca aattttaaat actgaatatt tgtttttttt cctattattt atttattctc
     5161 tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt
     5221 ttctctttcc gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt
     5281 tatttatctt ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac
     5341 acacaaaagg atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag
     5401 aaatatggag gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaactagc
     5461 tgccctcgat tgtctatctt ccattctcac caagtttcaa caactttatt cagtcattct
     5521 tctctggttc atttcccaac caatcttccc gaataaagcc catctgtcct ttcggccaca
     5581 ttcctcacct acatccgtac tccttatctc ccgttcttat ccagtctttt atcgtctctg
     5641 cgtctcgcac actgtctcgt cggtaatgta aaaaaaaaac aaaaaaaact tgaaaacttg
     5701 cggaaagatt tcctaataaa aggagacgaa tccggtagga aatcgacaag tctctgcttc
     5761 tcgaagacta ggtgagttac atttctttgg ccgaattatt ttgaaatcct aaatttgata
     5821 atttcagaga tgtctgtaaa acttgcgtgt tatgtgacgg cttcggtcac tgtcgccact
     5881 cttatggtat gcttcatgac catgtcaacc atctactcgg aggttgatgg attcagagag
     5941 aagcttgata ccgagatgaa tgtgttcaga gtgagttaaa ttatattttg aattttaaat
     6001 aatttttaat tttctagcaa tctaccaatg gattgtggaa ggacatagtt gtcatcggaa
     6061 gatctagcaa gcgtgtccgt tgtcaatatg aagagaccaa cgctacccca actccacatg
     6121 ctgatggatc cccatctgct ccaccaggtc aaccaccagc agttccacca gtcttcaacc
     6181 agccaaagac tccaaatgga gccaatggaa atggaccaac ctgcaactgc aatgctgata
     6241 acaagtgccc agctggacca tccggaccaa agggagttcc aggagttcca ggactcgacg
     6301 gagttccagg acttgacggt gttccaggag ttggagctga tgatatcgct ccacaacgcg
     6361 agtctgtcgg atgcttcact tgcccacaag gaccagttgg accaccagga gctcttggaa
     6421 gaccaggacc acgtggactt ccaggaccaa gaggacaaaa tggaaaccca ggaagagatg
     6481 gacaaccagg acatccagga gagcaaggat catccggtca aatcggaaag atcggagagc
     6541 caggaccacc aggagagaag ggacgcgacg ccgagcatcc aatcggaaga ccaggaccaa
     6601 agggaccaag aggagatcaa ggaccaacag gaccagctgg acagaacggt cttcacggac
     6661 caccaggaga gccaggaacc gttggaccag aaggaccatc tggaaagcaa ggacgtcaag
     6721 gaccagacgg aacccaggga gagactggac cagacggaag accaggaaag gatgccgagt
     6781 actgccagtg cccagacaag tctccaccat cagaggctgt caacgccaac cgtggataca
     6841 gaaatatcta aattgttggt gttttctaat aaaatatttg agattcagtg agactttttt
     6901 cttggcggca caataaagtt tcctcttttt gttgggtcac tcaacgaaca caatacaaat
     6961 atttgagcgc tcgcattttt ctcaaccttt cgggtttaaa gcacttcaaa atggaattga
     7021 agagagattg tgaaagtaca tcagacagaa tcacattttg atagcattca cttcactcag
     7081 atgcaaactt ttcagtgact ataaaaagtc ataaaataat aacattaaaa aagcaaataa
     7141 attagcgaga gaattttttg acaaaaagaa agaagagtga tagagaagaa gggaatgctt
     7201 gaaaggattt tgcatttatc ttaatcaccg tcttcgagaa gacgaaccat ggcgccagtt
     7261 tcagaatcca agtttctaat atagttcatc acaatgttga cgttggtcca acctccggct
     7321 tgcataatct ctgggatgga aactccagcg cgagccatgt cacgggcagc tccgacacgg
     7381 gcggaatgtc cagaccaagc caagtaacgt tgtccggaat catccttggc tccgtagatg
     7441 agacggtggg tagcttcgaa gattcctgaa aatttatttt cagcttattc ttagctaata
     7501 tgttgtttaa acttaccttc aagagctcga gtcgagagtt gtgatgttgc actcggggca
     7561 gcaactccgt tcttgcgaac acgacagaac aggtagttgt ttggatcatc ggcgactccg
     7621 gaaaccgaaa tccagcgctc aacgagcttg gtaactccaa gggagagcgc tttttcgact
     7681 ccggcagtcg agaccagcgt ttttgtacgt ccgatgtgga tgagcattct tcctccgtct
     7741 gtgcgactaa tatccttgac gcgaatgcgg gcaatttcgg cgatacggag aagtgtgttg
     7801 taagcaattc cgaggaaggc gaggtttcgg atgtcctggc aacgatcgga attttccata
     7861 agagaacgga cctgatcgaa gtcggttctg aaaatttcgt caagtcttag gattgaatac
     7921 attaatattt aaacttacct ttcgaaggcg agggcttgct tggctctctc tccagcatca
     7981 acattttcct tgcggatacg tctcattacg agagatacgg cattagaatc tgatgggcgt
     8041 ggaagtccag atcggcgatg gagcatattg agctgtccga gatgttgttg gatcgtcttc
     8101 accgccaatc cacgagcttg gaggtagagc aagtagtcac gaacgtcttc tggctcggct
     8161 gggaaccact tgcggttgtt aagcttgcac caggcagccc aagaacggca cactgagaga
     8221 agcatcttcc aggtgtgctc ggagaaggct tgtctgtcac ggaacatatc catgaggttc
     8281 ttgcgaactt catcacttgt cgcgtcaact ggaagtgctg gaagattttg atgaacagtg
     8341 agaagatttg acatattttc gaagtttttt agatgcacta gaacaaagcg tgttggcttc
     8401 ctctgagccc gctttcctta tatacccgca ttctgcagcc ttacagaatg ttctagaagg
     8461 tcctagatgc attcgtttga aaatactccc ggtgggtgca aagagacgca gacggaaaat
     8521 gtatctgggt ctctttattg tgtacactac ttttccatgt accgaatgtg agtcgccctc
     8581 cttttgcaac aagcagctcg aatgttctag aaaaaggtgg aaaatagtat aaataccgtt
     8641 gaaaataaat accgaacaac atttgctcta attgtgaaat tagaaatctt caaactataa
     8701 tcatgtcact ttaccactat ttccgtccag ctcaacgttc cgtttttggt gatcattttt
     8761 gctttcgtcg taaatctaca cacgcgtctc ttccgtgcga gagtccaagc cagcagccaa
     8821 attcgttgac tgagtattca acgtttatac gttgtcggca acgagaaata ggaaaatgca
     8881 tcgggaaatg ttcttttttc gattttttcc aaggttttga caaattttac cacgaatttt
     8941 gctatgtttt caattaaaaa atatgttatt caactgtttc tatgaggaaa ataaggcttt
     9001 gcatgtaatt ttcttattca gcataatttt taattaattt gaattttctg tcctaacgtt
     9061 tattttgttt tcttggttat gactgatctg aaattaattt ttgaatttta aggtaatatg
     9121 tcaggcggtg ccgcaagttt gtacaaaaaa gcaggctcca tgaaaaagcc tgaactcacc
     9181 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     9241 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     9301 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     9361 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     9421 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     9481 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     9541 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     9601 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     9661 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     9721 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     9781 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     9841 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     9901 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     9961 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
    10021 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
    10081 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
    10141 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagaccca gctttcttgt
    10201 acaaagtggg tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac
    10261 cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt
    10321 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
    10381 gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca
    10441 atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga aacatcatag
    10501 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
    10561 taataacttc gtatagcata cattatacga agttattttc agggagccgg atctgagctc
    10621 ttaagacgaa gtgagtttgc taccatcatg gaatcaggag gacatttcga caatgtcata
    10681 taatgtttga aaaatgattt taacaagggt ctcattcgag ataagcaatg ctctgagcaa
    10741 gtggctcgtc gtcttcaaga atctcttgtt gatattcatt tggatcctgt tttgcacgaa
    10801 gcatgtgcca tggatattca gagtttttgc aaaaaagttc cgccaggtca aacaatcttt
    10861 ttaattttta tatgatactt cgggtgtatt tttcttcttt ttcctgttca ttttcgcttt
    10921 tttctcactt ttttgtaaca aaatctctca cttttctgta agtcttaaca aaagttcaat
    10981 tggctcgatc aagtaaaatt tcggccgtat gaacgttttc aaaggttttc attttttttt
    11041 aagttcattc acaatatcat gaatctctcg tcctttggtt tccggtaagt agcgaaataa
    11101 tataagcata cttataaatg aaggaattga atatagaata aggaaagcat aactaccaat
    11161 aacggaatat agaggtaata ctgtaaatgt actgataacc accattacag tattcattga
    11221 atatgccaca gattgagcaa tactacgatg tttttgaggt accagttcag aggatataaa
    11281 ccacgaaatt ggaccaactc cagttctgaa atttcattat tcgattaaat caaaatatat
    11341 ttgaaaaacc tcacccataa gtataaccgt agatgatcaa tgcaactagg catccgtatt
    11401 tcatcgggtc aatcagaata cgtatttttg cgaagaatac gaatgcaatt agagctaaat
    11461 tattgagaaa tgtgaagaga aggagaagct tgcgtcgacc aactctgtaa acttattcat
    11521 acaattttgt cttcaaattt tgaacgttta aaactatctt attcaatgag ttagcgggtt
    11581 tttatagtga gttagttcac gattatctaa taaaactatg tccaaacatc ctaactgcga
    11641 ccgtattctc actaactcgt cttaaaaacg ttgagattat tttaaaccct tcttcagtac
    11701 aactacagta ctgatgcaca aatacaataa taataataca ttcagacata ctctgaacaa
    11761 tgtaacttgt ttttgttata ctgtggtaac atttattgaa atgaaatata accttacttt
    11821 tcaatcacaa ttccaccagt aattgttcca gaaacataag ctaaacccat tgcagttgta
    11881 ctccattcag caatttctga ctcaacattt gcattttcta gaaagaaagt agaggataaa
    11941 agaagagccc ataatc
//
