LOCUS       sequence-307907-       10721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector that can be modified to add gene(s) of interest to C. elegans
            ChrII:8420157 site via CRISPR with SEC selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Stevenson ZC, Moerdyk-Schauwecker MJ, Jamison B, Phillips PC
  TITLE     Rapid Self-Selecting and Clone-Free Integration of Transgenes into 
            Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans.
  JOURNAL   G3 (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400.
  PUBMED    32816924
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400."
COMMENT     Sequence Label: pMS4 (Empty insertion vector with SEC)
FEATURES             Location/Qualifiers
     source          1..10721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(151..739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(231..250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(910..1701,1702..1770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1533..1552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1771..1875)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1943..1961
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1999..2021)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    complement(3762..3795)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3805..3834
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(5651..5974)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    7892..7916
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7919..8944
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7919..8944
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8945..8969)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9322..9355)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggccctgtga aattgttatc cgctggtatc agctcactca aaggcggtaa tacggttatc
       61 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaagaccag
      121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1021 atctgacccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     1081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1321 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     1921 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     1981 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     2041 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     2101 tcggggctgg cttaactatg cggcatcaga gcagattgca ctgagagtgc accatatgcg
     2161 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     2221 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2281 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttccccagt
     2341 cacgacgtca cagcacgtgt gtttttgaac acggcgatat gtatctgtag atcatcccca
     2401 attttcaaca atgaatctaa atactgaact ttttgtgtaa tctttagtgc agaaaaatga
     2461 ccaaaatcag cgattttccg attataccca aaatatctca gctcaatgag cacctataaa
     2521 aatgtaaaaa taaagaaagt agtcgaacca tattttgtaa aggctaaaaa actgaataaa
     2581 tttttatcca tccagcgcgg cgttacaatc cgtggcccat gtcacgcacg gctttcaaag
     2641 aaaaaaaaac agtctgtttg cgcgacatcg atctggcaaa aatcctaaaa acgactatgc
     2701 gtggctcact ttatattaat atgaaaaata caaacttggt ggtgcaggtg ctgcagaggc
     2761 catcgatgaa gaagtggagg ccttgttgtc gatctacaaa attgtatggt tgtttgagat
     2821 tttccttgaa acgctctcaa tcttggatat gatataaatt ctggataaga tatgatggat
     2881 atgatctatg tttgatagag tgcatttgaa aggataacgg ccctgcagac atttttgaaa
     2941 aaataacaaa catttaattt tctcactcgt ttaggctatt cccctatttt tgatattcct
     3001 tcgcacatat gaaaactact ttttttcgaa actgttaact ccagaattta taaatctata
     3061 gcccttactt gattatttat tatcatggtt actcacctca tgtctctttc tcttttcaag
     3121 aagtcttcgt tcatatctgt tgtctctttc ctccttttga atctctgctt tgctcttcgc
     3181 cattgtttcc tgaaaataat gtaacttgaa ttgtgtaata tactttttta atttgaattt
     3241 ggcttgtaac gcggaatcac tacgtgcggg atcatttctt actagaaaac ccagaaaatg
     3301 ccatatttca cttatctcgg ggtcatttct aattagaaaa gctacaaaaa ccatttctaa
     3361 ttacggtgac tttagaactg gtcgaaatag ctaattagaa atgggttttt gctaattaga
     3421 ggtgactcta tgttatctga gtaagcctcg tttgtatgta aactcacagt actaataaat
     3481 gcaagacacc cgggtttgtc tagatatgaa ataattgaaa tatcaattct gacagacaat
     3541 aatggtaatc ttgataagga gttccacgcc caggagaaca cgttagtttt cttgtttttg
     3601 attgcgtgcg ttattttgga gaaaaactcg attttttaca aaataatttt ttgaaaggaa
     3661 cactgttcaa taagttttgt cttttttctc agttgtgata cggtttttta ttcttttttg
     3721 tagttataca gaagaccgcc tgcaggagct gtaagtttaa aataacttcg tatagcatac
     3781 attatacgaa gttattttca gggagagcaa aagcttatct ccgaggagga cctctaagga
     3841 tgatcgacgc caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc
     3901 ctattattta tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc
     3961 caaatctaaa catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc
     4021 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga
     4081 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat
     4141 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata
     4201 gaggaggaag gaaactagct gccctcgatt gtctatcttc cattctcacc aagtttcaac
     4261 aactttattc agtcattctt ctctggttca tttcccaacc aatcttcccg aataaagccc
     4321 atctgtcctt tcggccacat tcctcaccta catccgtact ccttatctcc cgttcttatc
     4381 cagtctttta tcgtctctgc gtctcgcaca ctgtctcgtc ggtaatgtaa aaaaaaaaca
     4441 aaaaaaactt gaaaacttgc ggaaagattt cctaataaaa ggagacgaat ccggtaggaa
     4501 atcgacaagt ctctgcttct cgaagactag gtgagttaca tttctttggc cgaattattt
     4561 tgaaatccta aatttgataa tttcagagat gtctgtaaaa cttgcgtgtt atgtgacggc
     4621 ttcggtcact gtcgccactc ttatggtatg cttcatgacc atgtcaacca tctactcgga
     4681 ggttgatgga ttcagagaga agcttgatac cgagatgaat gtgttcagag tgagttaaat
     4741 tatattttga attttaaata atttttaatt ttctagcaat ctaccaatgg attgtggaag
     4801 gacatagttg tcatcggaag atctagcaag cgtgtccgtt gtcaatatga agagaccaac
     4861 gctaccccaa ctccacatgc tgatggatcc ccatctgctc caccaggtca accaccagca
     4921 gttccaccag tcttcaacca gccaaagact ccaaatggag ccaatggaaa tggaccaacc
     4981 tgcaactgca atgctgataa caagtgccca gctggaccat ccggaccaaa gggagttcca
     5041 ggagttccag gactcgacgg agttccagga cttgacggtg ttccaggagt tggagctgat
     5101 gatatcgctc cacaacgcga gtctgtcgga tgcttcactt gcccacaagg accagttgga
     5161 ccaccaggag ctcttggaag accaggacca cgtggacttc caggaccaag aggacaaaat
     5221 ggaaacccag gaagagatgg acaaccagga catccaggag agcaaggatc atccggtcaa
     5281 atcggaaaga tcggagagcc aggaccacca ggagagaagg gacgcgacgc cgagcatcca
     5341 atcggaagac caggaccaaa gggaccaaga ggagatcaag gaccaacagg accagctgga
     5401 cagaacggtc ttcacggacc accaggagag ccaggaaccg ttggaccaga aggaccatct
     5461 ggaaagcaag gacgtcaagg accagacgga acccagggag agactggacc agacggaaga
     5521 ccaggaaagg atgccgagta ctgccagtgc ccagacaagt ctccaccatc agaggctgtc
     5581 aacgccaacc gtggatacag aaatatctaa attgttggtg ttttctaata aaatatttga
     5641 gattcagtga gacttttttc ttggcggcac aataaagttt cctctttttg ttgggtcact
     5701 caacgaacac aatacaaata tttgagcgct cgcatttttc tcaacctttc gggtttaaag
     5761 cacttcaaaa tggaattgaa gagagattgt gaaagtacat cagacagaat cacattttga
     5821 tagcattcac ttcactcaga tgcaaacttt tcagtgacta taaaaagtca taaaataata
     5881 acattaaaaa agcaaataaa ttagcgagag aattttttga caaaaagaaa gaagagtgat
     5941 agagaagaag ggaatgcttg aaaggatttt gcatttatct taatcaccgt cttcgagaag
     6001 acgaaccatg gcgccagttt cagaatccaa gtttctaata tagttcatca caatgttgac
     6061 gttggtccaa cctccggctt gcataatctc tgggatggaa actccagcgc gagccatgtc
     6121 acgggcagct ccgacacggg cggaatgtcc agaccaagcc aagtaacgtt gtccggaatc
     6181 atccttggct ccgtagatga gacggtgggt agcttcgaag attcctgaaa atttattttc
     6241 agcttattct tagctaatat gttgtttaaa cttaccttca agagctcgag tcgagagttg
     6301 tgatgttgca ctcggggcag caactccgtt cttgcgaaca cgacagaaca ggtagttgtt
     6361 tggatcatcg gcgactccgg aaaccgaaat ccagcgctca acgagcttgg taactccaag
     6421 ggagagcgct ttttcgactc cggcagtcga gaccagcgtt tttgtacgtc cgatgtggat
     6481 gagcattctt cctccgtctg tgcgactaat atccttgacg cgaatgcggg caatttcggc
     6541 gatacggaga agtgtgttgt aagcaattcc gaggaaggcg aggtttcgga tgtcctggca
     6601 acgatcggaa ttttccataa gagaacggac ctgatcgaag tcggttctga aaatttcgtc
     6661 aagtcttagg attgaataca ttaatattta aacttacctt tcgaaggcga gggcttgctt
     6721 ggctctctct ccagcatcaa cattttcctt gcggatacgt ctcattacga gagatacggc
     6781 attagaatct gatgggcgtg gaagtccaga tcggcgatgg agcatattga gctgtccgag
     6841 atgttgttgg atcgtcttca ccgccaatcc acgagcttgg aggtagagca agtagtcacg
     6901 aacgtcttct ggctcggctg ggaaccactt gcggttgtta agcttgcacc aggcagccca
     6961 agaacggcac actgagagaa gcatcttcca ggtgtgctcg gagaaggctt gtctgtcacg
     7021 gaacatatcc atgaggttct tgcgaacttc atcacttgtc gcgtcaactg gaagtgctgg
     7081 aagattttga tgaacagtga gaagatttga catattttcg aagtttttta gatgcactag
     7141 aacaaagcgt gttggcttcc tctgagcccg ctttccttat atacccgcat tctgcagcct
     7201 tacagaatgt tctagaaggt cctagatgca ttcgtttgaa aatactcccg gtgggtgcaa
     7261 agagacgcag acggaaaatg tatctgggtc tctttattgt gtacactact tttccatgta
     7321 ccgaatgtga gtcgccctcc ttttgcaaca agcagctcga atgttctaga aaaaggtgga
     7381 aaatagtata aataccgttg aaaataaata ccgaacaaca tttgctctaa ttgtgaaatt
     7441 agaaatcttc aaactataat catgtcactt taccactatt tccgtccagc tcaacgttcc
     7501 gtttttggtg atcatttttg ctttcgtcgt aaatctacac acgcgtctct tccgtgcgag
     7561 agtccaagcc agcagccaaa ttcgttgact gagtattcaa cgtttatacg ttgtcggcaa
     7621 cgagaaatag gaaaatgcat cgggaaatgt tcttttttcg attttttcca aggttttgac
     7681 aaattttacc acgaattttg ctatgttttc aattaaaaaa tatgttattc aactgtttct
     7741 atgaggaaaa taaggctttg catgtaattt tcttattcag cataattttt aattaatttg
     7801 aattttctgt cctaacgttt attttgtttt cttggttatg actgatctga aattaatttt
     7861 tgaattttaa ggtaatatgt caggcggtgc cgcaagtttg tacaaaaaag caggctccat
     7921 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     7981 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     8041 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     8101 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     8161 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     8221 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     8281 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     8341 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     8401 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     8461 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     8521 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     8581 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     8641 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     8701 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     8761 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     8821 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     8881 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     8941 atagacccag ctttcttgta caaagtgggt ccaattactc ttcaacatcc ctacatgctc
     9001 tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct
     9061 ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag
     9121 aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt
     9181 ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa
     9241 tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc
     9301 agtttatgac cgcaattttt aataacttcg tatagcatac attatacgaa gttattttca
     9361 gggagccgga tctgagctct taagacgaag tgagtttgct accatcatgg aatcaggagg
     9421 acatttcgac aatgtcatat aatgtttgaa aaatgatttt aacaagggtc tcattcgaga
     9481 taagcaatgc tctgagcaag tggctcgtcg tcttcaagaa tctcttgttg atattcattt
     9541 ggatcctgtt ttgcacgaag catgtgccat ggatattcag agtttttgca aaaaagttcc
     9601 gccaggtcaa acaatctttt taatttttat atgatacttc gggtgtattt ttcttctttt
     9661 tcctgttcat tttcgctttt ttctcacttt tttgtaacaa aatctctcac ttttctgtaa
     9721 gtcttaacaa aagttcaatt ggctcgatca agtaaaattt cggccgtatg aacgttttca
     9781 aaggttttca ttttttttta agttcattca caatatcatg aatctctcgt cctttggttt
     9841 ccggtaagta gcgaaataat ataagcatac ttataaatga aggaattgaa tatagaataa
     9901 ggaaagcata actaccaata acggaatata gaggtaatac tgtaaatgta ctgataacca
     9961 ccattacagt attcattgaa tatgccacag attgagcaat actacgatgt ttttgaggta
    10021 ccagttcaga ggatataaac cacgaaattg gaccaactcc agttctgaaa tttcattatt
    10081 cgattaaatc aaaatatatt tgaaaaacct cacccataag tataaccgta gatgatcaat
    10141 gcaactaggc atccgtattt catcgggtca atcagaatac gtatttttgc gaagaatacg
    10201 aatgcaatta gagctaaatt attgagaaat gtgaagagaa ggagaagctt gcgtcgacca
    10261 actctgtaaa cttattcata caattttgtc ttcaaatttt gaacgtttaa aactatctta
    10321 ttcaatgagt tagcgggttt ttatagtgag ttagttcacg attatctaat aaaactatgt
    10381 ccaaacatcc taactgcgac cgtattctca ctaactcgtc ttaaaaacgt tgagattatt
    10441 ttaaaccctt cttcagtaca actacagtac tgatgcacaa atacaataat aataatacat
    10501 tcagacatac tctgaacaat gtaacttgtt tttgttatac tgtggtaaca tttattgaaa
    10561 tgaaatataa ccttactttt caatcacaat tccaccagta attgttccag aaacataagc
    10621 taaacccatt gcagttgtac tccattcagc aatttctgac tcaacatttg cattttctag
    10681 aaagaaagta gaggataaaa gaagagccca taatccgaca g
//
