LOCUS       sequence-307918-        9606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of synthetic protein composed of eGFP and 5 repeats of 
            BRD4 bromodomain #1 with N140A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pMTTH+eGFP-bromo5(N140A)
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(85..90,91..106,107..113)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    121..137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     126..148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             207..1307
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1164..1173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1281..1304
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1356..1367
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1380..1400
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(1410..1412,1413..1415,1416..2126)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1410..1412,1413..1415,1416..2126)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1455..1476)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1716..1735)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2063..2084
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4302..4322
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4323..4340
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4353..4370)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4353..4369)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4362..4384)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4548..4565)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4718..4804
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4896..4923
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4942..5033
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5034..5102,5103..5894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5252..5271)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5936..6449)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6131..6152)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6343..6362
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6560..7148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7049..7068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7302..7319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7425..7444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7585..7607
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7601..7792)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8288..8365
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8366..9448
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8385..8404)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
       61 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
      121 ttgtgagcgg ataacaattt cacacaggaa acagccagtc cgtttaggtg ttttcacgag
      181 cacttcacca acaaggacca tagattatga aaactgaaga aggtaaactg gtaatctgga
      241 ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata
      301 ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg
      361 cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg
      421 ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc
      481 cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg
      541 aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag
      601 agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc
      661 tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt
      721 atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg
      781 gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact
      841 ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg
      901 catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca
      961 agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac gccgccagtc
     1021 cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat gaaggtctgg
     1081 aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt
     1141 tggcgaaaga tccacgtatt gccgccacca tggaaaacgc ccagaaaggt gaaatcatgc
     1201 cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg atcaacgccg
     1261 ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactaat tcgagctcga
     1321 acaacaacaa caataacaat aacaacaacc tcgggatcga gggaaggatt tcagaatttg
     1381 aaaacctgta ttttcagggc catatggcca tggtgagcaa gggcgaggag ctgttcaccg
     1441 gggtggtgcc tatcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1501 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1561 ccggcaagct gccagtgccg tggcctaccc tcgtgaccac cctgacctac ggcgtgcagt
     1621 gcttcagccg ctacccagac cacatgaagc agcacgactt cttcaagtcc gccatgccgg
     1681 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1741 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1801 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1861 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1921 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cctatcggcg
     1981 acggcccagt gctgctgccg gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     2041 accctaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     2101 ctctcggcat ggacgagctg tacaagggtg gcagcggcgg gtcgggcggg agcggcggca
     2161 gtggaggaag tgttgcctcg actaatcctc caccgccgga gaccagtaac ccaaacaagc
     2221 ctaaacgtca aacgaaccaa ctccaatatc tgttgcgtgt tgttttgaaa acattgtgga
     2281 aacaccaatt tgcatggcct tttcaacaac cggttgacgc ggtgaagtta aaccttccgg
     2341 attattacaa aattatcaaa actccgatgg atatggggac tatcaaaaaa cggttggaga
     2401 acaactacta ctggaatgcc caagagtgta ttcaagattt caacaccatg ttcacaaact
     2461 gttatatcta cgcaaaacca ggagacgata tcgttctgat ggccgaggcc cttgagaaat
     2521 tatttttaca gaaaatcaac gaactcccga cagaggaggg cgggtccggt ggaagtggcg
     2581 gtagtggtgg gagtggtgga tcagccgcca gcacaaaccc acctccgcct gaaacgtcca
     2641 acccgaacaa accgaaacgg cagactaacc agctgcagta cttacttcgg gtagtattga
     2701 agacactgtg gaagcatcag ttcgcctggc cgttccaaca gccagtagat gcagttaaac
     2761 ttaatttacc tgattattat aaaatcatta aaacacctat ggatatggga actattaaga
     2821 aacgcctgga gaataactat tattggaatg ctcaagaatg tatccaggat ttcaatacca
     2881 tgtttacgaa ctgttacatt tacgcgaaac ctggtgatga tatcgtgctt atggcagaag
     2941 cactggaaaa gctgtttttg caaaaaatta atgagctgcc tactgaggaa gggggaagcg
     3001 ggggctcggg ggggtcaggc ggttctggag gatctgccgc atccactaac ccgcctccgc
     3061 cagagacgag taatccgaac aagccgaagc gccaaacgaa tcaattacag tatcttcttc
     3121 gtgtcgtgtt aaaaacgctt tggaagcacc agtttgcgtg gccattccag cagcctgtgg
     3181 atgcggttaa attaaatctc ccggactatt acaagatcat caaaacccct atggatatgg
     3241 gcacgatcaa gaagcgcctc gaaaacaatt actattggaa tgcgcaagaa tgcatccagg
     3301 actttaatac gatgtttact aactgctata tttatgctaa accgggcgat gatattgtat
     3361 tgatggcgga agctcttgaa aaattgtttc ttcagaagat caatgaactc cctaccgaag
     3421 agggaggttc gggagggagc ggtggatctg gaggctccgg agggtctgct gcgtctacca
     3481 atccgccacc accggaaact agcaatccta acaaaccaaa acgtcagacc aatcaactgc
     3541 aatacctctt acgcgttgtg cttaaaaccc tctggaaaca ccagtttgcc tggcctttcc
     3601 agcaaccagt ggacgccgtg aagttgaacc tgccagacta ctataagatc attaagacgc
     3661 caatggatat gggtacaatt aagaagcggt tggaaaacaa ctattattgg aatgcacagg
     3721 aatgtatcca agacttcaat actatgttta caaattgcta catctatgcc aagccgggag
     3781 atgacattgt tttaatggct gaggcattag aaaagttgtt cttacaaaag attaatgaat
     3841 tgcctacgga agaaggtggc tctgggggct caggtggtag tgggggttcc gggggctccg
     3901 cagcctcgac caaccctcct ccacctgaga cctctaatcc aaataaacct aagcggcaaa
     3961 ccaatcagct ccagtacctg cttcgcgtag tactgaagac cttatggaaa catcaattcg
     4021 cttggccgtt tcaacagcct gttgatgctg ttaagctgaa cttgcctgat tactacaaga
     4081 ttatcaagac cccgatggac atggggacga ttaaaaagcg gcttgaaaat aattattatt
     4141 ggaacgccca ggagtgcatt caggatttta acacgatgtt caccaattgt tatatttacg
     4201 caaagccagg ggacgacatc gtactcatgg cagaggcact tgagaaactt ttcctccaga
     4261 aaattaacga gttaccaacc gaggagggct cgagcggatc cgaaaacctg tattttcagg
     4321 gccatcatca tcatcatcac tgaaagcttg gcactggccg tcgttttaca acgtcgtgac
     4381 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4441 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4501 ggcgaatggc agcttggctg ttttggcgga tgagataaga ttttcagcct gatacagatt
     4561 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     4621 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     4681 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     4741 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     4801 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     4861 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     4921 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc
     4981 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5041 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5101 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5161 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5221 gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     5281 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5341 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5401 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5461 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5521 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5581 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5641 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5701 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5761 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5821 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5881 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttacccc
     5941 ggttgataat cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt
     6001 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     6061 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt
     6121 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6181 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag
     6241 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6301 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6361 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     6421 cgcgcttaat gcgccgctac agggcgcgta aaaggatcta ggtgaagatc ctttttgata
     6481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7201 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7261 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7321 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7381 gcatgtggtg cactctcagt acaccgcatg tggtgcactc tcagtacaat ctgctctgat
     7441 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     7501 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7561 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     7621 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac
     7681 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     7741 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     7801 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     7861 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     7921 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     7981 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     8041 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     8101 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     8161 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     8221 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg
     8281 aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
     8341 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
     8401 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
     8461 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
     8521 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
     8581 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
     8641 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
     8701 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
     8761 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
     8821 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
     8881 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
     8941 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
     9001 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     9061 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     9121 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     9181 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     9241 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     9301 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     9361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9421 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc
     9481 attaggcaca attctcatgt ttgacagctt atcatcgact gcacggtgca ccaatgcttc
     9541 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcata
     9601 attcgt
//
