LOCUS       sequence-307925-        4749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express His tagged hnRNPA2 RRM2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ryan VH, Watters S, Amaya J, Khatiwada B, Venditti V, Naik MT, Fawzi
            NL
  TITLE     Weak binding to the A2RE RNA rigidifies hnRNPA2 RRMs and reduces 
            liquid-liquid phase separation and aggregation.
  JOURNAL   Nucleic Acids Res. 2020 Sep 1. pii: 5900113. doi: 
            10.1093/nar/gkaa710.
  PUBMED    32870271
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Sep 1. pii: 5900113. doi: 10.1093/nar/gkaa710."
COMMENT     Sequence Label: hnRNPA2_RRM2
FEATURES             Location/Qualifiers
     source          1..4749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     208..227
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        208..226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    227..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             312..329
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             330..350
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      1111..1158
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1381..1400)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        1467..1558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1559..2368
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2451..3533
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2470..2489)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      3619..4207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4108..4127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4361..4378
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4476..4493
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4477..4493
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat
       61 aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata catgtcaacg
      121 ataatacaaa atataataca aactataaga tgttatcagt atttattatg catttagaat
      181 aaattttgtg tcgcccttcc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      241 ataacaattc ccctctagaa ataattttgt ttaacttttg aaggagatat accatgggta
      301 gcgacaagat tcaccaccat catcatcatg agaatttgta ctttcagggc catggtgctc
      361 acgtcaccgt caaaaagctg ttcgtgggtg gtatcaagga agataccgag gaacaccact
      421 tgcgcgacta cttcgaggaa tatggcaaaa tcgataccat tgagatcatt acggaccgtc
      481 aaagcggcaa aaagcgtggt ttcggctttg ttacgtttga cgatcacgac ccggttgaca
      541 aaatcgtgct gcaaaagtac cacaccatta acggccacaa cgccgaggtg cgtaaagcgc
      601 tgagccgtca agagatgcaa gaagtgcagt cctctcgcag ctagcgtggt ggcaactttg
      661 gtttcggtga tagccgcggt ggtggcggca acttcggtcc gggtccgggc agcaatttcc
      721 gtggtggttc cgacggttac ggtagcggtc gcggctttgg cgatggctat aatggttacg
      781 gtggtggtcc gggcggcggc aatttcggtg gtagcccggg ttacggcggt ggtcgcggcg
      841 gttatggcgg tggcggtcca ggctacggta accagggtgg tggttatggt ggtggctacg
      901 ataactatgg cggtggtaac tatggtagcg gcaattacaa tgattttggc aactacaacc
      961 agcaaccgag caattacggc ccgatgaaat ccggcaattt tggcggttcc cgtaatatgg
     1021 gtggtccgta tggtggcggt aactacggcc cgggtggtag cggtggttct ggcggctatg
     1081 gtggccgcag ccgttactaa ctcgagcccc ctagcataac cccttggggc ctctaaacgg
     1141 gtcttgaggg gttttttgcc cctgagacgc gtcaatcgag ttcgtaccta agggcgacac
     1201 cccctaatta gcccgggcga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     1261 gcctggcagt tccctactct cgcatgggga gtccccacac taccatcggc gctacggcgt
     1321 ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa
     1381 caaggggtgt tatgagccat attcaggtat aaatgggctc gcgataatgt tcagaattgg
     1441 ttaattggtt gtaacactga cccctatttg tttatttttc taaatacatt caaatatgta
     1501 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatat
     1561 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1621 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1681 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1741 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc cacttccgac
     1801 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1861 aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1921 gctggcagtg ttcctgcgcc ggttgcactc gattcctgtt tgtaattgtc cttttaacag
     1981 cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2041 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2101 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2161 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     2221 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2281 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcaatt
     2341 tcatttgatg ctcgatgagt ttttctaagc ggcgcgccat cgaatggcgc aaaacctttc
     2401 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat atgaaaccag
     2461 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     2521 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     2581 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     2641 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     2701 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     2761 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     2821 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     2881 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     2941 aggacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     3001 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     3061 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     3121 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     3181 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     3241 ttggtgcgga tatctcggta gtgggatacg acgataccga agatagctca tgttatatcc
     3301 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     3361 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgcca gtctcactgg
     3421 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3481 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgactcatga
     3541 ccaaaatccc ttaacgtgag ttacgcgcgc gtcgttccac tgagcgtcag accccgtaga
     3601 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3661 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3721 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3781 gtagttagcc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3841 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3901 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3961 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4021 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4081 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4141 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4201 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4261 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4321 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4381 agcggaaggc gagagtaggg aactgccagg catcaaacta agcagaaggc ccctgacgga
     4441 tggccttttt gcgtttctac aaactctttc tgtgttgtaa aacgacggcc agtcttaagc
     4501 tcgggccccc tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac
     4561 ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt
     4621 aagggcgcct gtcactttgc ttgatatatg agaattattt aaccttataa atgagaaaaa
     4681 agcaacgcac tttaaataag atacgttgct ttttcgattg atgaacacct ataattaaac
     4741 tattcatct
//
