LOCUS       sequence-307927-        8289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of renilla luciferase and firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni
            G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, 
            Kempa S, Huttelmaier S, Selbach M, Landthaler M
  TITLE     HDLBP binds ER-targeted mRNAs by multivalent interactions to promote
            protein synthesis of transmembrane and secreted proteins.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7.
  PUBMED    35585045
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2727. doi"; pages: " 
            10.1038/s41467-022-30322-7"
COMMENT     Sequence Label: pFRTpsiCHECK
FEATURES             Location/Qualifiers
     source          1..8289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        66..257
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(89..109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      274..409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          493..625
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     608..627
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        670..688
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             698..1633
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1692..1740
                     /note="synthetic polyadenylation signal"
     promoter        1749..2500
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2530..2539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2536..4188
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3495..3503
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(4234..4251)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4240..4464
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4597..4616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4748..4795
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4803..5825
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4803..5825
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5955..6076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5992..6011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6046..6065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6125..6141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6125..6141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6138..6160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6149..6165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6173..6179,6180..6197,6198..6203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6218..6239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6356..6373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6527..7115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6607..6626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7286..8077,8078..8146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7909..7928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8147..8251)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgggagatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
       61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
      481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      661 tagagtactt aatacgactc actataggct agccaccatg gcttccaagg tgtacgaccc
      721 cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa
      781 cgtgctggac tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat
      841 ttttctgcat ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc ctcacatcga
      901 gcccgtggct agatgcatca tccctgatct gatcggaatg ggtaagtccg gcaagagcgg
      961 gaatggctca tatcgcctcc tggatcacta caagtacctc accgcttggt tcgagctgct
     1021 gaaccttcca aagaaaatca tctttgtggg ccacgactgg ggggcttgtc tggcctttca
     1081 ctactcctac gagcaccaag acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga
     1141 cgtgatcgag tcctgggacg agtggcctga catcgaggag gatatcgccc tgatcaagag
     1201 cgaagagggc gagaaaatgg tgcttgagaa taacttcttc gtcgagacca tgctcccaag
     1261 caagatcatg cggaaactgg agcctgagga gttcgctgcc tacctggagc cattcaagga
     1321 gaagggcgag gttagacggc ctaccctctc ctggcctcgc gagatccctc tcgttaaggg
     1381 aggcaagccc gacgtcgtcc agattgtccg caactacaac gcctaccttc gggccagcga
     1441 cgatctgcct aagatgttca tcgagtccga ccctgggttc ttttccaacg ctattgtcga
     1501 gggagctaag aagttcccta acaccgagtt cgtgaaggtg aagggcctcc acttcagcca
     1561 ggaggacgct ccagatgaaa tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa
     1621 gaacgagcag taattctagg cgatcgctcg agcccgggaa ttcgtttaaa cctagagcgg
     1681 ccgctggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     1741 aggatctaaa tgagtcttcg gacctcgcgg gggccgctta agcggtggtt agggtttgtc
     1801 tgacgcgggg ggagggggaa ggaacgaaac actctcattc ggaggcggct cggggtttgg
     1861 tcttggtggc cacgggcacg cagaagagcg ccgcgatcct cttaagcacc cccccgccct
     1921 ccgtggaggc gggggtttgg tcggcgggtg gtaactggcg ggccgctgac tcgggcgggt
     1981 cgcgcgcccc agagtgtgac cttttcggtc tgctcgcaga cccccgggcg gcgccgccgc
     2041 ggcggcgacg ggctcgctgg gtcctaggct ccatggggac cgtatacgtg gacaggctct
     2101 ggagcatccg cacgactgcg gtgatattac cggagacctt ctgcgggacg agccgggtca
     2161 cgcggctgac gcggagcgtc cgttgggcga caaacaccag gacggggcac aggtacacta
     2221 tcttgtcacc cggaggcgcg agggactgca ggagcttcag ggagtggcgc agctgcttca
     2281 tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg
     2341 tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca
     2401 cgcagatgca gtcggggcgg cgcggtccca ggtccacttc gcatattaag gtgacgcgtg
     2461 tggcctcgaa caccgagcga ccctgcagcg acccgcttaa aagcttggca ttccggtact
     2521 gttggtaaag ccaccatggc cgatgctaag aacattaaga agggccctgc tcccttctac
     2581 cctctggagg atggcaccgc tggcgagcag ctgcacaagg ccatgaagag gtatgccctg
     2641 gtgcctggca ccattgcctt caccgatgcc cacattgagg tggacatcac ctatgccgag
     2701 tacttcgaga tgtctgtgcg cctggccgag gccatgaaga ggtacggcct gaacaccaac
     2761 caccgcatcg tggtgtgctc tgagaactct ctgcagttct tcatgccagt gctgggcgcc
     2821 ctgttcatcg gagtggccgt ggcccctgct aacgacattt acaacgagcg cgagctgctg
     2881 aacagcatgg gcatttctca gcctaccgtg gtgttcgtgt ctaagaaggg cctgcagaag
     2941 atcctgaacg tgcagaagaa gctgcctatc atccagaaga tcatcatcat ggactctaag
     3001 accgactacc agggcttcca gagcatgtac acattcgtga catctcatct gcctcctggc
     3061 ttcaacgagt acgacttcgt gccagagtct ttcgacaggg acaaaaccat tgccctgatc
     3121 atgaacagct ctgggtctac cggcctgcct aagggcgtgg ccctgcctca tcgcaccgcc
     3181 tgtgtgcgct tctctcacgc ccgcgaccct attttcggca accagatcat ccccgacacc
     3241 gctattctga gcgtggtgcc attccaccac ggcttcggca tgttcaccac cctgggctac
     3301 ctgatttgcg gctttcgggt ggtgctgatg taccgcttcg aggaggagct gttcctgcgc
     3361 agcctgcaag actacaaaat tcagtctgcc ctgctggtgc caaccctgtt cagcttcttc
     3421 gctaagagca ccctgatcga caagtacgac ctgtctaacc tgcacgagat tgcctctggc
     3481 ggcgccccac tgtctaagga ggtgggcgaa gccgtggcca agcgctttca tctgccaggc
     3541 atccgccagg gctacggcct gaccgagaca accagcgcca ttctgattac cccagagggc
     3601 gacgacaagc ctggcgccgt gggcaaggtg gtgccattct tcgaggccaa ggtggtggac
     3661 ctggacaccg gcaagaccct gggagtgaac cagcgcggcg agctgtgtgt gcgcggccct
     3721 atgattatgt ccggctacgt gaataaccct gaggccacaa acgccctgat cgacaaggac
     3781 ggctggctgc actctggcga cattgcctac tgggacgagg acgagcactt cttcatcgtg
     3841 gaccgcctga agtctctgat caagtacaag ggctaccagg tggccccagc cgagctggag
     3901 tctatcctgc tgcagcaccc taacattttc gacgccggag tggccggcct gcccgacgac
     3961 gatgccggcg agctgcctgc cgccgtcgtc gtgctggaac acggcaagac catgaccgag
     4021 aaggagatcg tggactatgt ggccagccag gtgacaaccg ccaagaagct gcgcggcgga
     4081 gtggtgttcg tggacgaggt gcccaagggc ctgaccggca agctggacgc ccgcaagatc
     4141 cgcgagatcc tgatcaaggc taagaaaggc ggcaagatcg ccgtgtaata attctagtcg
     4201 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4261 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4321 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4381 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4441 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4501 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4741 gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg
     4801 ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     4861 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     4921 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     4981 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     5041 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     5101 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     5161 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     5221 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     5281 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     5341 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     5401 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     5461 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     5521 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     5581 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     5641 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     5701 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     5761 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     5821 aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     5881 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5941 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6001 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6061 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6121 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6181 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6241 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8281 tcgacggat
//
