LOCUS       sequence-307932-       12288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Human TMPRSS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12288)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Bla-Ak-TMPRSS2
FEATURES             Location/Qualifiers
     source          1..12288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        198..1376
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          429..1367
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1324..1344
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1424..1541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3127..3694
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3145..3696
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3289..3306)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3516..3535
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3699..4097
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(4142..4158)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4143..4159)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4172..4760
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4225..4245)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4643..4654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4904..4937)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4957..5137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5162..5180)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5163..5180)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5470..5489)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5703..5722)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5822..5844
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5882..5900)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5968..6072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6073..6141,6142..6933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6291..6310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7104..7692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7593..7612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7846..7863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7933..7953)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7938..8267
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8118..8253
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9401..9466
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9596..9616
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10041..10175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10078..10097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10132..10151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10343..10976
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11023..11148
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11641..11874
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12059..12103
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
       61 atctcgacgg tatcgatcac gagactagcc tcgaccatcg atgtcgacga taagctttgc
      121 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      181 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      241 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      301 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      361 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      421 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      481 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      541 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      601 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
      661 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
      721 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
      781 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
      841 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
      901 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
      961 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1021 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1081 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1141 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1201 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1261 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1321 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1381 tttcgacatt taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg
     1441 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1501 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc
     1561 tcgaggttta aacccgggag caggcacatg gctttgaact cagggtcacc accagctatt
     1621 ggaccttact atgaaaacca tggataccaa ccggaaaacc cctatcccgc acagcccact
     1681 gtggtcccca ctgtctacga ggtgcatccg gctcagtact acccgtcccc cgtgccccag
     1741 tacgccccga gggtcctgac gcaggcttcc aaccccgtcg tctgcacgca gcccaaatcc
     1801 ccatccggga cagtgtgcac ctcaaagact aagaaagcac tgtgcatcac cttgaccctg
     1861 gggaccttcc tcgtgggagc tgcgctggcc gctggcctac tctggaagtt catgggcagc
     1921 aagtgctcca actctgggat agagtgcgac tcctcaggta cctgcatcaa cccctctaac
     1981 tggtgtgatg gcgtgtcaca ctgccccggc ggggaggacg agaatcggtg tgttcgcctc
     2041 tacggaccaa acttcatcct tcaggtgtac tcatctcaga ggaagtcctg gcaccctgtg
     2101 tgccaagacg actggaacga gaactacggg cgggcggcct gcagggacat gggctataag
     2161 aataattttt actctagcca aggaatagtg gatgacagcg gatccaccag ctttatgaaa
     2221 ctgaacacaa gtgccggcaa tgtcgatatc tataaaaaac tgtaccacag tgatgcctgt
     2281 tcttcaaaag cagtggtttc tttacgctgt atagcctgcg gggtcaactt gaactcaagc
     2341 cgccagagca ggatcgtggg cggcgagagc gcgctcccgg gggcctggcc ctggcaggtc
     2401 agcctgcacg tccagaacgt ccacgtgtgc ggaggctcca tcatcacccc cgagtggatc
     2461 gtgacagccg cccactgcgt ggaaaaacct cttaacaatc catggcattg gacggcattt
     2521 gcggggattt tgagacaatc tttcatgttc tatggagccg gataccaagt agaaaaagtg
     2581 atttctcatc caaattatga ctccaagacc aagaacaatg acattgcgct gatgaagctg
     2641 cagaagcctc tgactttcaa cgacctagtg aaaccagtgt gtctgcccaa cccaggcatg
     2701 atgctgcagc cagaacagct ctgctggatt tccgggtggg gggccaccga ggagaaaggg
     2761 aagacctcag aagtgctgaa cgctgccaag gtgcttctca ttgagacaca gagatgcaac
     2821 agcagatatg tctatgacaa cctgatcaca ccagccatga tctgtgccgg cttcctgcag
     2881 gggaacgtcg attcttgcca gggtgacagt ggagggcctc tggtcacttc gaagaacaat
     2941 atctggtggc tgatagggga tacaagctgg ggttctggct gtgccaaagc ttacagacca
     3001 ggagtgtacg ggaatgtgat ggtattcacg gactggattt atcgacaaat gagggcagac
     3061 ggctagggat ccatatgcgg accgtacgta ctagtggcca cggctgatca tacgggctgc
     3121 aggaattccg cccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     3181 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     3241 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     3301 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     3361 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     3421 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     3481 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     3541 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     3601 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
     3661 ggacgtggtt ttcctttgaa aaacacgatg ataataccat ggccaagcct ttgtctcaag
     3721 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     3781 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     3841 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     3901 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     3961 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     4021 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     4081 atgtgtggga gggctaacta gctagtcgag ctcaacttcg aattcgatat caagcttatc
     4141 gcgataccgt cgacctcgag ggaattccga taatcaacct ctggattaca aaatttgtga
     4201 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4261 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4321 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4381 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4441 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4501 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4561 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4621 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4681 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4741 cctttgggcc gcctccccgc atcgggaatt cgagctcggt acctttaaga ccaatgactt
     4801 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4861 ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt cgtatagcat
     4921 acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4981 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5041 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5101 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     5161 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     5221 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     5281 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     5341 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     5401 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     5461 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     5521 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     5581 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     5641 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     5701 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5761 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5821 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5881 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5941 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6001 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6061 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6121 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6181 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6241 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6301 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6361 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6421 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6481 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6541 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6601 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6661 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6721 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6781 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6841 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6901 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7801 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7861 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7921 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7981 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8041 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8101 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8161 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8221 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     8281 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     8341 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     8401 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     8461 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     8521 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     8581 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     8641 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     8701 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     8761 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     8821 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     8881 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     8941 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     9001 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     9061 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     9121 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     9181 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     9241 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     9301 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
     9361 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     9421 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     9481 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     9541 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     9601 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     9661 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     9721 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     9781 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     9841 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     9901 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     9961 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    10021 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    10081 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10141 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10201 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    10261 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10321 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    10381 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    10441 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    10501 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    10561 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    10621 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    10681 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    10741 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    10801 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10861 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10921 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    10981 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    11041 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    11101 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    11161 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    11221 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    11281 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    11341 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    11401 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    11461 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
    11521 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    11581 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    11641 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    11701 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    11761 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    11821 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    11881 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    11941 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    12001 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    12061 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    12121 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    12181 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    12241 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccga
//
