LOCUS       sequence-307933-       14731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Human ACE2 and TMPRSS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14731)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 14731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Bla-Ak-ACE2-TMPRSS2
FEATURES             Location/Qualifiers
     source          1..14731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     92..111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             303..936
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    983..1108
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1601..1834
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2019..2063
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2446..3624
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2677..3615
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3572..3592
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3672..3789
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7818..8385
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7836..8387
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7980..7997)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8207..8226
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8390..8788
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(8833..8849)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8834..8850)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    8863..9451
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8916..8936)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9334..9345)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9595..9628)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9648..9828
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9853..9871)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9854..9871)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10161..10180)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10394..10413)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10513..10535
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10573..10591)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10659..10763
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10764..10832,10833..11624)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10982..11001)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11795..12383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12537..12554
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12624..12644)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12629..12958
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12809..12944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          14092..14157
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14287..14307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
       61 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
      121 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
      181 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
      241 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
      301 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
      361 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
      421 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
      481 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
      541 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
      601 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
      661 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
      721 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
      781 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      841 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      901 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      961 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1021 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1081 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1141 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1201 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1261 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1321 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1381 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1441 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1501 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1561 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1621 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1681 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1741 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1801 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1861 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1921 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1981 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     2041 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2101 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2161 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2221 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2281 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     2341 gaccatcgat gtcgacgata agctttgcaa agatggataa agttttaaac agagaggaat
     2401 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2461 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2521 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2581 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2641 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     2701 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     2761 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     2821 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     2881 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     2941 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3001 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3061 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3121 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3181 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3241 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3301 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3361 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3421 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3481 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3541 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3601 tttcttccat ttcaggtgtc gtgaggaatt tcgacattta aatttaatta atctcgacgg
     3661 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3721 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3781 caaaatttta tcgatcacga gactagcctc gaggtttaaa cccgggagcg ccaccatgtc
     3841 aagctcttcc tggctccttc tcagccttgt tgctgtaact gctgctcagt ccaccattga
     3901 ggaacaggcc aagacatttt tggacaagtt taaccacgaa gccgaagacc tgttctatca
     3961 aagttcactt gcttcttgga attataacac caatattact gaagagaatg tccaaaacat
     4021 gaataatgct ggggacaaat ggtctgcctt tttaaaggaa cagtccacac ttgcccaaat
     4081 gtatccacta caagaaattc agaatctcac agtcaagctt cagctgcagg ctcttcagca
     4141 aaatgggtct tcagtgctct cagaagacaa gagcaaacgg ttgaacacaa ttctaaatac
     4201 aatgagcacc atctacagta ctggaaaagt ttgtaaccca gataatccac aagaatgctt
     4261 attacttgaa ccaggtttga atgaaataat ggcaaacagt ttagactaca atgagaggct
     4321 ctgggcttgg gaaagctgga gatctgaggt cggcaagcag ctgaggccat tatatgaaga
     4381 gtatgtggtc ttgaaaaatg agatggcaag agcaaatcat tatgaggact atggggatta
     4441 ttggagagga gactatgaag taaatggggt agatggctat gactacagcc gcggccagtt
     4501 gattgaagat gtggaacata cctttgaaga gattaaacca ttatatgaac atcttcatgc
     4561 ctatgtgagg gcaaagttga tgaatgccta tccttcctat atcagtccaa ttggatgcct
     4621 ccctgctcat ttgcttggtg atatgtgggg tagattttgg acaaatctgt actctttgac
     4681 agttcccttt ggacagaaac caaacataga tgttactgat gcaatggtgg accaggcctg
     4741 ggatgcacag agaatattca aggaggccga gaagttcttt gtatctgttg gtcttcctaa
     4801 tatgactcaa ggattctggg aaaattccat gctaacggac ccaggaaatg ttcagaaagc
     4861 agtctgccat cccacagctt gggacctggg gaagggcgac ttcaggatcc ttatgtgcac
     4921 aaaggtgaca atggacgact tcctgacagc tcatcatgag atggggcata tccagtatga
     4981 tatggcatat gctgcacaac cttttctgct aagaaatgga gctaatgaag gattccatga
     5041 agctgttggg gaaatcatgt cactttctgc agccacacct aagcatttaa aatccattgg
     5101 tcttctgtca cccgattttc aagaagacaa tgaaacagaa ataaacttcc tgctcaaaca
     5161 agcactcacg attgttggga ctctgccatt tacttacatg ttagagaagt ggaggtggat
     5221 ggtctttaaa ggggaaattc ccaaagacca gtggatgaaa aagtggtggg agatgaagcg
     5281 agagatagtt ggggtggtgg aacctgtgcc ccatgatgaa acatactgtg accccgcatc
     5341 tctgttccat gtttctaatg attactcatt cattcgatat tacacaagga ccctttacca
     5401 attccagttt caagaagcac tttgtcaagc agctaaacat gaaggccctc tgcacaaatg
     5461 tgacatctca aactctacag aagctggaca gaaactgttc aatatgctga ggcttggaaa
     5521 atcagaaccc tggaccctag cattggaaaa tgttgtagga gcaaagaaca tgaatgtaag
     5581 gccactgctc aactactttg agcccttatt tacctggctg aaagaccaga acaagaattc
     5641 ttttgtggga tggagtaccg actggagtcc atatgcagac caaagcatca aagtgaggat
     5701 aagcctaaaa tcagctcttg gagataaagc atatgaatgg aacgacaatg aaatgtacct
     5761 gttccgatca tctgttgcat atgctatgag gcagtacttt ttaaaagtaa aaaatcagat
     5821 gattcttttt ggggaggagg atgtgcgagt ggctaatttg aaaccaagaa tctcctttaa
     5881 tttctttgtc actgcaccta aaaatgtgtc tgatatcatt cctagaactg aagttgaaaa
     5941 ggccatcagg atgtcccgga gccgtatcaa tgatgctttc cgtctgaatg acaacagcct
     6001 agagtttctg gggatacagc caacacttgg acctcctaac cagccccctg tttccatatg
     6061 gctgattgtt tttggagttg tgatgggagt gatagtggtt ggcattgtca tcctgatctt
     6121 cactgggatc agagatcgga agaagaaaaa taaagcaaga agtggagaaa atccttatgc
     6181 ctccatcgat attagcaaag gagaaaataa tccaggattc caaaacactg atgatgttca
     6241 gacctccttt tagcgcgcct gcaggatcag caggcaccat ggctttgaac tcagggtcac
     6301 caccagctat tggaccttac tatgaaaacc atggatacca accggaaaac ccctatcccg
     6361 cacagcccac tgtggtcccc actgtctacg aggtgcatcc ggctcagtac tacccgtccc
     6421 ccgtgcccca gtacgccccg agggtcctga cgcaggcttc caaccccgtc gtctgcacgc
     6481 agcccaaatc cccatccggg acagtgtgca cctcaaagac taagaaagca ctgtgcatca
     6541 ccttgaccct ggggaccttc ctcgtgggag ctgcgctggc cgctggccta ctctggaagt
     6601 tcatgggcag caagtgctcc aactctggga tagagtgcga ctcctcaggt acctgcatca
     6661 acccctctaa ctggtgtgat ggcgtgtcac actgccccgg cggggaggac gagaatcggt
     6721 gtgttcgcct ctacggacca aacttcatcc ttcaggtgta ctcatctcag aggaagtcct
     6781 ggcaccctgt gtgccaagac gactggaacg agaactacgg gcgggcggcc tgcagggaca
     6841 tgggctataa gaataatttt tactctagcc aaggaatagt ggatgacagc ggatccacca
     6901 gctttatgaa actgaacaca agtgccggca atgtcgatat ctataaaaaa ctgtaccaca
     6961 gtgatgcctg ttcttcaaaa gcagtggttt ctttacgctg tatagcctgc ggggtcaact
     7021 tgaactcaag ccgccagagc aggatcgtgg gcggcgagag cgcgctcccg ggggcctggc
     7081 cctggcaggt cagcctgcac gtccagaacg tccacgtgtg cggaggctcc atcatcaccc
     7141 ccgagtggat cgtgacagcc gcccactgcg tggaaaaacc tcttaacaat ccatggcatt
     7201 ggacggcatt tgcggggatt ttgagacaat ctttcatgtt ctatggagcc ggataccaag
     7261 tagaaaaagt gatttctcat ccaaattatg actccaagac caagaacaat gacattgcgc
     7321 tgatgaagct gcagaagcct ctgactttca acgacctagt gaaaccagtg tgtctgccca
     7381 acccaggcat gatgctgcag ccagaacagc tctgctggat ttccgggtgg ggggccaccg
     7441 aggagaaagg gaagacctca gaagtgctga acgctgccaa ggtgcttctc attgagacac
     7501 agagatgcaa cagcagatat gtctatgaca acctgatcac accagccatg atctgtgccg
     7561 gcttcctgca ggggaacgtc gattcttgcc agggtgacag tggagggcct ctggtcactt
     7621 cgaagaacaa tatctggtgg ctgatagggg atacaagctg gggttctggc tgtgccaaag
     7681 cttacagacc aggagtgtac gggaatgtga tggtattcac ggactggatt tatcgacaaa
     7741 tgagggcaga cggctaggga tccatatgcg gaccgtacgt actagtggcc acggctgatc
     7801 atacgggctg caggaattcc gccccccccc ccctaacgtt actggccgaa gccgcttgga
     7861 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     7921 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     7981 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     8041 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     8101 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     8161 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     8221 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     8281 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
     8341 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatacca tggccaagcc
     8401 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     8461 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     8521 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     8581 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     8641 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     8701 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     8761 gccctctggt tatgtgtggg agggctaact agctagtcga gctcaacttc gaattcgata
     8821 tcaagcttat cgcgataccg tcgacctcga gggaattccg ataatcaacc tctggattac
     8881 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8941 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9001 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9061 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9121 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9181 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9241 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     9301 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9361 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9421 agtcggatct ccctttgggc cgcctccccg catcgggaat tcgagctcgg tacctttaag
     9481 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9541 ggaagggcta attcactccc aacgaagaca agatgggatc aattcaccat gggaataact
     9601 tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt
     9661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     9841 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     9901 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     9961 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
    10021 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
    10081 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
    10141 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
    10201 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
    10261 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
    10321 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
    10381 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
    10441 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    10501 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    10561 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    10621 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10681 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10741 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10801 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10861 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10921 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10981 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    11041 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    11101 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    11161 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    11221 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    11281 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    11341 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    11401 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    11461 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    11521 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11581 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11641 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11701 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11761 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11821 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11881 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11941 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    12001 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    12061 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    12121 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    12181 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    12241 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtata
    12301 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    12361 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    12421 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    12481 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    12541 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    12601 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
    12661 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
    12721 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    12781 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    12841 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    12901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    12961 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
    13021 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
    13081 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
    13141 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
    13201 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
    13261 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
    13321 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
    13381 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
    13441 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
    13501 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
    13561 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
    13621 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
    13681 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
    13741 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
    13801 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
    13861 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
    13921 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
    13981 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
    14041 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
    14101 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
    14161 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
    14221 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
    14281 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
    14341 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
    14401 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
    14461 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
    14521 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    14581 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    14641 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    14701 gaaacataaa atgaatgcaa ttgttgttgt t
//
