LOCUS       sequence-307939-       13175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13175)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 13175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: 
            pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
FEATURES             Location/Qualifiers
     source          1..13175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3431..3451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7010..7019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7742..7751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8222..8269
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8288..8353
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8360..8927
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8521..8538)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8748..8767
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8947..9546
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8947..8966)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9443..9463
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9565..10153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9618..9638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10036..10047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10228..10461
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10580..10598)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10666..10770
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10771..10839,10840..11631)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10989..11008)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11802..12390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12291..12310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12544..12561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12801..12822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12837..12842,12843..12860,12861..12867)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12875..12891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12880..12902
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12899..12915
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12899..12915
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12965..12984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
     3421 agctagcatg cccaaaaaga agaggaaagt gaacatcccc gctctggtgg aaaaccagaa
     3481 gaagtacttt ggcacctaca gcgtgatggc catgctgaac gctcagaccg tgctggacca
     3541 catccagaag gtggccgata ttgagggcga gcagaacgag aacaacgaga atctgtggtt
     3601 tcaccccgtg atgagccacc tgtacaacgc caagaacggc tacgacaagc agcccgagaa
     3661 aaccatgttc atcatcgagc ggctgcagag ctacttccca ttcctgaaga tcatggccga
     3721 gaaccagaga gagtacagca acggcaagta caagcagaac cgcgtggaag tgaacagcaa
     3781 cgacatcttc gaggtgctga agcgcgcctt cggcgtgctg aagatgtaca gggacctgac
     3841 caacgcctac aagacctacg aggaaaagct gaacgacggc tgcgagttcc tgaccagcac
     3901 agagcaacct ctgagcggca tgatcaacaa ctactacaca gtggccctgc ggaacatgaa
     3961 cgagagatac ggctacaaga cagaggacct ggccttcatc caggacaagc ggttcaagtt
     4021 cgtgaaggac gcctacggca agaaaaagtc ccaagtgaat accggattct tcctgagcct
     4081 gcaggactac aacggcgaca cacagaagaa gctgcacctg agcggagtgg gaatcgccct
     4141 gctgatctgc ctgttcctgg acaagcagta catcaacatc tttctgagca ggctgcccat
     4201 cttctccagc tacaatgccc agagcgagga acggcggatc atcatcagat ccttcggcat
     4261 caacagcatc aagctgccca aggaccggat ccacagcgag aagtccaaca agagcgtggc
     4321 catggatatg ctcaacgaag tgaagcggtg ccccgacgag ctgttcacaa cactgtctgc
     4381 cgagaagcag tcccggttca gaatcatcag cgacgaccac aatgaagtgc tgatgaagcg
     4441 gagcagcgac agattcgtgc ctctgctgct gcagtatatc gattacggca agctgttcga
     4501 ccacatcagg ttccacgtga acatgggcaa gctgagatac ctgctgaagg ccgacaagac
     4561 ctgcatcgac ggccagacca gagtcagagt gatcgagcag cccctgaacg gcttcggcag
     4621 actggaagag gccgagacaa tgcggaagca agagaacggc accttcggca acagcggcat
     4681 ccggatcaga gacttcgaga acatgaagcg ggacgacgcc aatcctgcca actatcccta
     4741 catcgtggac acctacacac actacatcct ggaaaacaac aaggtcgaga tgtttatcaa
     4801 cgacaaagag gacagcgccc cactgctgcc cgtgatcgag gatgatagat acgtggtcaa
     4861 gacaatcccc agctgccgga tgagcaccct ggaaattcca gccatggcct tccacatgtt
     4921 tctgttcggc agcaagaaaa ccgagaagct gatcgtggac gtgcacaacc ggtacaagag
     4981 actgttccag gccatgcaga aagaagaagt gaccgccgag aatatcgcca gcttcggaat
     5041 cgccgagagc gacctgcctc agaagatcct ggatctgatc agcggcaatg cccacggcaa
     5101 ggatgtggac gccttcatca gactgaccgt ggacgacatg ctgaccgaca ccgagcggag
     5161 aatcaagaga ttcaaggacg accggaagtc cattcggagc gccgacaaca agatgggaaa
     5221 gagaggcttc aagcagatct ccacaggcaa gctggccgac ttcctggcca aggacatcgt
     5281 gctgtttcag cccagcgtga acgatggcga gaacaagatc accggcctga actaccggat
     5341 catgcagagc gccattgccg tgtacgatag cggcgacgat tacgaggcca agcagcagtt
     5401 caagctgatg ttcgagaagg cccggctgat cggcaagggc acaacagagc ctcatccatt
     5461 tctgtacaag gtgttcgccc gcagcatccc cgccaatgcc gtcgagttct acgagcgcta
     5521 cctgatcgag cggaagttct acctgaccgg cctgtccaac gagatcaaga aaggcaacag
     5581 agtggatgtg cccttcatcc ggcgggacca gaacaagtgg aaaacacccg ccatgaagac
     5641 cctgggcaga atctacagcg aggatctgcc cgtggaactg cccagacaga tgttcgacaa
     5701 tgagatcaag tcccacctga agtccctgcc acagatggaa ggcatcgact tcaacaatgc
     5761 caacgtgacc tatctgatcg ccgagtacat gaagagagtg ctggacgacg acttccagac
     5821 cttctaccag tggaaccgca actaccggta catggacatg cttaagggcg agtacgacag
     5881 aaagggctcc ctgcagcact gcttcaccag cgtggaagag agagaaggcc tctggaaaga
     5941 gcgggcctcc agaacagagc ggtacagaaa gcaggccagc aacaagatcc gcagcaaccg
     6001 gcagatgaga aacgccagca gcgaagagat cgagacaatc ctggataagc ggctgagcaa
     6061 cagccggaac gagtaccaga aaagcgagaa agtgatccgg cgctacagag tgcaggatgc
     6121 cctgctgttt ctgctggcca aaaagaccct gaccgaactg gccgatttcg acggcgagag
     6181 gttcaaactg aaagaaatca tgcccgacgc cgagaaggga atcctgagcg agatcatgcc
     6241 catgagcttc accttcgaga aaggcggcaa gaagtacacc atcaccagcg agggcatgaa
     6301 gctgaagaac tacggcgact tctttgtgct ggctagcgac aagaggatcg gcaacctgct
     6361 ggaactcgtg ggcagcgaca tcgtgtccaa agaggatatc atggaagagt tcaacaaata
     6421 cgaccagtgc aggcccgaga tcagctccat cgtgttcaac ctggaaaagt gggccttcga
     6481 cacatacccc gagctgtctg ccagagtgga ccgggaagag aaggtggact tcaagagcat
     6541 cctgaaaatc ctgctgaaca acaagaacat caacaaagag cagagcgaca tcctgcggaa
     6601 gatccggaac gccttcgatg ccaacaatta ccccgacaaa ggcgtggtgg aaatcaaggc
     6661 cctgcctgag atcgccatga gcatcaagaa ggcctttggg gagtacgcca tcatgaagga
     6721 gggagctcta gaggggacta gtggaggtgg atccatggtg tccaagggcg aagaggacaa
     6781 catggccagc ctgcctgcca cccacgagct gcacatcttc ggcagcatca acggcgtgga
     6841 cttcgacatg gtgggacagg gcaccggcaa ccccaacgac ggctacgagg aactgaacct
     6901 gaagtccaca aagggcgacc tgcagttcag cccctggatt ctggtgcccc acatcggcta
     6961 cggcttccac cagtacctgc cctaccccga cggcatgagc ccttttcagg ccgccatggt
     7021 ggatggcagc ggctaccagg tgcaccggac catgcagttt gaggacggcg ccagcctgac
     7081 cgtgaactac cggtacacat acgagggcag ccacatcaag ggcgaggccc aagtgaaggg
     7141 cacaggcttt ccagccgacg gccccgtgat gaccaatagc ctgacagccg ccgactggtg
     7201 cagaagcaag aaaacctacc ccaatgacaa gaccatcatc agcaccttca agtggtccta
     7261 caccaccggc aatggcaagc ggtacagaag caccgcccgg accacctaca ccttcgccaa
     7321 acccatggcc gccaactacc tgaagaacca gcctatgtac gtgttccgca agaccgagct
     7381 gaagcactcc aagacagaac tgaacttcaa agagtggcag aaagccttca ccgacgtgat
     7441 gggcatggac gagctgtaca agtcgaattc tgcagtcgac ggtaccatgg tgtccaaggg
     7501 cgaagaggac aacatggcca gcctgcctgc cacccacgag ctgcacatct tcggcagcat
     7561 caacggcgtg gacttcgaca tggtgggaca gggcaccggc aaccccaacg acggctacga
     7621 ggaactgaac ctgaagtcca caaagggcga cctgcagttc agcccctgga ttctggtgcc
     7681 ccacatcggc tacggcttcc accagtacct gccctacccc gacggcatga gcccttttca
     7741 ggccgccatg gtggatggca gcggctacca ggtgcaccgg accatgcagt ttgaggacgg
     7801 cgccagcctg accgtgaact accggtacac atacgagggc agccacatca agggcgaggc
     7861 ccaagtgaag ggcacaggct ttccagccga cggccccgtg atgaccaata gcctgacagc
     7921 cgccgactgg tgcagaagca agaaaaccta ccccaatgac aagaccatca tcagcacctt
     7981 caagtggtcc tacaccaccg gcaatggcaa gcggtacaga agcaccgccc ggaccaccta
     8041 caccttcgcc aaacccatgg ccgccaacta cctgaagaac cagcctatgt acgtgttccg
     8101 caagaccgag ctgaagcact ccaagacaga actgaacttc aaagagtggc agaaagcctt
     8161 caccgacgtg atgggcatgg acgagctgta caagtccgga ctcagatctt gcctacatat
     8221 gaagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaac aacgcgtgca
     8281 gctaagcgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     8341 tgacgatgac aagtaaggat ccctcccccc cccctaacgt tactggccga agccgcttgg
     8401 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     8461 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     8521 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     8581 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     8641 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     8701 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     8761 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     8821 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     8881 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagga tccaccggag
     8941 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     9001 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     9061 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     9121 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     9181 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     9241 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     9301 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     9361 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     9421 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     9481 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     9541 gcctgacgcc catcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     9601 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     9661 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     9721 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     9781 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     9841 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     9901 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9961 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    10021 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    10081 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    10141 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
    10201 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    10261 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10321 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10381 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10441 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
    10501 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
    10561 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    10621 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10681 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10741 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    10801 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    10861 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10921 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    10981 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
    11041 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    11101 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    11161 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    11221 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    11281 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
    11341 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    11401 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    11461 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    11521 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    11581 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    11641 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    11701 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    11761 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    11821 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    11881 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11941 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    12001 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    12061 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    12121 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    12181 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    12241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    12301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    12361 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    12421 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    12481 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    12541 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    12601 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
    12661 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    12721 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
    12781 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    12841 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    12901 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
    12961 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    13021 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    13081 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    13141 tgtatttgtt aaatatgtac tacaaactta gtagt
//
