LOCUS       sequence-307944-       12826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 12826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: pHAGE-NLS-dPspCas13b-3xmRuby3-NLS
FEATURES             Location/Qualifiers
     source          1..12826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3431..3451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6746..6755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6752..7462
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     CDS             8933..8980
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     9094..9114
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9216..9804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9269..9289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9687..9698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9879..10112
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10231..10249)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10317..10421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10422..10490,10491..11282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10640..10659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11453..12041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11942..11961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12195..12212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12452..12473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12488..12493,12494..12511,12512..12518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12526..12542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12531..12553
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12550..12566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12550..12566
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12616..12635
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
     3421 agctagcatg cccaaaaaga agaggaaagt gaacatcccc gctctggtgg aaaaccagaa
     3481 gaagtacttt ggcacctaca gcgtgatggc catgctgaac gctcagaccg tgctggacca
     3541 catccagaag gtggccgata ttgagggcga gcagaacgag aacaacgaga atctgtggtt
     3601 tcaccccgtg atgagccacc tgtacaacgc caagaacggc tacgacaagc agcccgagaa
     3661 aaccatgttc atcatcgagc ggctgcagag ctacttccca ttcctgaaga tcatggccga
     3721 gaaccagaga gagtacagca acggcaagta caagcagaac cgcgtggaag tgaacagcaa
     3781 cgacatcttc gaggtgctga agcgcgcctt cggcgtgctg aagatgtaca gggacctgac
     3841 caacgcctac aagacctacg aggaaaagct gaacgacggc tgcgagttcc tgaccagcac
     3901 agagcaacct ctgagcggca tgatcaacaa ctactacaca gtggccctgc ggaacatgaa
     3961 cgagagatac ggctacaaga cagaggacct ggccttcatc caggacaagc ggttcaagtt
     4021 cgtgaaggac gcctacggca agaaaaagtc ccaagtgaat accggattct tcctgagcct
     4081 gcaggactac aacggcgaca cacagaagaa gctgcacctg agcggagtgg gaatcgccct
     4141 gctgatctgc ctgttcctgg acaagcagta catcaacatc tttctgagca ggctgcccat
     4201 cttctccagc tacaatgccc agagcgagga acggcggatc atcatcagat ccttcggcat
     4261 caacagcatc aagctgccca aggaccggat ccacagcgag aagtccaaca agagcgtggc
     4321 catggatatg ctcaacgaag tgaagcggtg ccccgacgag ctgttcacaa cactgtctgc
     4381 cgagaagcag tcccggttca gaatcatcag cgacgaccac aatgaagtgc tgatgaagcg
     4441 gagcagcgac agattcgtgc ctctgctgct gcagtatatc gattacggca agctgttcga
     4501 ccacatcagg ttccacgtga acatgggcaa gctgagatac ctgctgaagg ccgacaagac
     4561 ctgcatcgac ggccagacca gagtcagagt gatcgagcag cccctgaacg gcttcggcag
     4621 actggaagag gccgagacaa tgcggaagca agagaacggc accttcggca acagcggcat
     4681 ccggatcaga gacttcgaga acatgaagcg ggacgacgcc aatcctgcca actatcccta
     4741 catcgtggac acctacacac actacatcct ggaaaacaac aaggtcgaga tgtttatcaa
     4801 cgacaaagag gacagcgccc cactgctgcc cgtgatcgag gatgatagat acgtggtcaa
     4861 gacaatcccc agctgccgga tgagcaccct ggaaattcca gccatggcct tccacatgtt
     4921 tctgttcggc agcaagaaaa ccgagaagct gatcgtggac gtgcacaacc ggtacaagag
     4981 actgttccag gccatgcaga aagaagaagt gaccgccgag aatatcgcca gcttcggaat
     5041 cgccgagagc gacctgcctc agaagatcct ggatctgatc agcggcaatg cccacggcaa
     5101 ggatgtggac gccttcatca gactgaccgt ggacgacatg ctgaccgaca ccgagcggag
     5161 aatcaagaga ttcaaggacg accggaagtc cattcggagc gccgacaaca agatgggaaa
     5221 gagaggcttc aagcagatct ccacaggcaa gctggccgac ttcctggcca aggacatcgt
     5281 gctgtttcag cccagcgtga acgatggcga gaacaagatc accggcctga actaccggat
     5341 catgcagagc gccattgccg tgtacgatag cggcgacgat tacgaggcca agcagcagtt
     5401 caagctgatg ttcgagaagg cccggctgat cggcaagggc acaacagagc ctcatccatt
     5461 tctgtacaag gtgttcgccc gcagcatccc cgccaatgcc gtcgagttct acgagcgcta
     5521 cctgatcgag cggaagttct acctgaccgg cctgtccaac gagatcaaga aaggcaacag
     5581 agtggatgtg cccttcatcc ggcgggacca gaacaagtgg aaaacacccg ccatgaagac
     5641 cctgggcaga atctacagcg aggatctgcc cgtggaactg cccagacaga tgttcgacaa
     5701 tgagatcaag tcccacctga agtccctgcc acagatggaa ggcatcgact tcaacaatgc
     5761 caacgtgacc tatctgatcg ccgagtacat gaagagagtg ctggacgacg acttccagac
     5821 cttctaccag tggaaccgca actaccggta catggacatg cttaagggcg agtacgacag
     5881 aaagggctcc ctgcagcact gcttcaccag cgtggaagag agagaaggcc tctggaaaga
     5941 gcgggcctcc agaacagagc ggtacagaaa gcaggccagc aacaagatcc gcagcaaccg
     6001 gcagatgaga aacgccagca gcgaagagat cgagacaatc ctggataagc ggctgagcaa
     6061 cagccggaac gagtaccaga aaagcgagaa agtgatccgg cgctacagag tgcaggatgc
     6121 cctgctgttt ctgctggcca aaaagaccct gaccgaactg gccgatttcg acggcgagag
     6181 gttcaaactg aaagaaatca tgcccgacgc cgagaaggga atcctgagcg agatcatgcc
     6241 catgagcttc accttcgaga aaggcggcaa gaagtacacc atcaccagcg agggcatgaa
     6301 gctgaagaac tacggcgact tctttgtgct ggctagcgac aagaggatcg gcaacctgct
     6361 ggaactcgtg ggcagcgaca tcgtgtccaa agaggatatc atggaagagt tcaacaaata
     6421 cgaccagtgc aggcccgaga tcagctccat cgtgttcaac ctggaaaagt gggccttcga
     6481 cacatacccc gagctgtctg ccagagtgga ccgggaagag aaggtggact tcaagagcat
     6541 cctgaaaatc ctgctgaaca acaagaacat caacaaagag cagagcgaca tcctgcggaa
     6601 gatccggaac gccttcgatg ccaacaatta ccccgacaaa ggcgtggtgg aaatcaaggc
     6661 cctgcctgag atcgccatga gcatcaagaa ggcctttggg gagtacgcca tcatgaagga
     6721 gggagctcta gaggcgctac cggtcgccac catggtgtct aagggcgaag agctgatcaa
     6781 ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg
     6841 cacaggtgaa ggagaaggca gaccgtacga gggagtgcaa accatgagga tcaaagtcat
     6901 cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag
     6961 ccgtaccttt atcaagtacc cggccgacat ccctgatttc tttaaacagt cctttcctga
     7021 gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcacgca
     7081 ggacaccagc cttgaggatg gcgagctcgt ctacaacgtc aaggtcagag gggtaaactt
     7141 tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat
     7201 gatgtatcca gcagatggtg gtctgagagg atacactgac atcgcactga aagttgatgg
     7261 tggtggccat ctgcactgca acttcgtgac aacttacagg tcaaaaaaga ccgtcgggaa
     7321 catcaagatg cccggtgtcc atgccgttga tcaccgcctg gaaaggatcg aggagagtga
     7381 caatgaaacc tacgtagtgc aaagagaagt ggcagttgcc aaatacagca accttggtgg
     7441 tggcatggac gagctgtaca agtccggact cagatctgtg agcaagggcg aggagctgat
     7501 caaggaaaat atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa
     7561 atgcacaggt gaaggagaag gcagaccgta cgagggagtg caaaccatga ggatcaaagt
     7621 catcgaggga ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg
     7681 cagccgtacc tttatcaagt acccggccga catccctgat ttctttaaac agtcctttcc
     7741 tgagggtttt acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcac
     7801 gcaggacacc agccttgagg atggcgagct cgtctacaac gtcaaggtca gaggggtaaa
     7861 ctttccctcc aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga
     7921 gatgatgtat ccagcagatg gtggtctgag aggatacact gacatcgcac tgaaagttga
     7981 tggtggtggc catctgcact gcaacttcgt gacaacttac aggtcaaaaa agaccgtcgg
     8041 gaacatcaag atgcccggtg tccatgccgt tgatcaccgc ctggaaagga tcgaggagag
     8101 tgacaatgaa acctacgtag tgcaaagaga agtggcagtt gccaaataca gcaaccttgg
     8161 tggtggcatg gacgagctgt acaagaagct tgtgagcaag ggcgaggagc tgatcaagga
     8221 aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac
     8281 aggtgaagga gaaggcagac cgtacgaggg agtgcaaacc atgaggatca aagtcatcga
     8341 gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt atggcagccg
     8401 tacctttatc aagtacccgg ccgacatccc tgatttcttt aaacagtcct ttcctgaggg
     8461 ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg tcacgcagga
     8521 caccagcctt gaggatggcg agctcgtcta caacgtcaag gtcagagggg taaactttcc
     8581 ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata cagagatgat
     8641 gtatccagca gatggtggtc tgagaggata cactgacatc gcactgaaag ttgatggtgg
     8701 tggccatctg cactgcaact tcgtgacaac ttacaggtca aaaaagaccg tcgggaacat
     8761 caagatgccc ggtgtccatg ccgttgatca ccgcctggaa aggatcgagg agagtgacaa
     8821 tgaaacctac gtagtgcaaa gagaagtggc agttgccaaa tacagcaacc ttggtggtgg
     8881 catggacgag ctgtacaagt ccggactcag atctcgacgg taccgccata tgaagcgacc
     8941 tgccgccaca aagaaggctg gacaggctaa gaagaagaaa tagcgtctcg cccgaccacc
     9001 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     9061 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     9121 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     9181 gcaagcccgg tgcctgacgc ccatcgatag atcctaatca acctctggat tacaaaattt
     9241 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     9301 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     9361 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     9421 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     9481 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     9541 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     9601 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     9661 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     9721 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     9781 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc
     9841 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     9901 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     9961 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10021 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10081 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
    10141 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga
    10201 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
    10261 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
    10321 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10381 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10441 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10501 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10561 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10621 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
    10681 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10741 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10801 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10861 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10921 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10981 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11041 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11101 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11161 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11221 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11281 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11341 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11401 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11461 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11521 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11581 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11641 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11701 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11761 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11821 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11881 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11941 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12001 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12061 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12121 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12241 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
    12301 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    12361 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
    12421 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    12481 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    12541 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
    12601 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    12661 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    12721 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    12781 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
