LOCUS       sequence-307955-       14700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 cloning vector for one or several guide RNAs, already 
            contains Cas9 and Bar selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT,
            Stuttmann J, Marillonnet S
  TITLE     High-efficiency genome editing in plants mediated by a Cas9 gene 
            containing multiple introns.
  JOURNAL   Plant Commun. 2020 Nov 23;2(2):100135. doi: 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8.
  PUBMED    33898975
REFERENCE   2  (bases 1 to 14700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2020-11-23"; volume: "2(2):100135. doi"; pages: " 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8"
COMMENT     Sequence Label: pAGM65879
FEATURES             Location/Qualifiers
     source          1..14700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             367..996
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1425..2498
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2820..2924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2925..3719
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3890..4478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4379..4398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4614..4638
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4803..4982
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5018..5073
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      5634..6341
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             8044..8064
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13797..13817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      13844..14096
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    14170..14191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14206..14211,14212..14229,14230..14236)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14244..14260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14249..14271
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14268..14284
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14268..14284
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             14280..14603
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    14297..14353
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(14354..14371)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14354..14370)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14363..14385)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(14579..14598)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgccgtgcg
      181 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
      241 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
      301 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
      361 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
      421 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
      481 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
      541 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
      601 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
      661 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
      721 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
      781 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
      841 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
      901 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
      961 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     1021 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     1081 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     1141 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     1201 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     1261 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     1321 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     1381 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     1441 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     1501 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     1561 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgtcgt
     1621 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     1681 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     1741 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     1801 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     1861 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     1921 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     1981 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     2041 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     2101 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     2161 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     2221 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     2281 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     2341 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     2401 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     2461 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     2521 aaattgccct agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat agcacgtaca
     2581 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     2641 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     2701 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     2761 tgtctggcca gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg ctccgagtgc
     2821 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2881 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
     2941 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     3001 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     3061 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     3121 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     3181 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     3241 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     3301 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     3361 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
     3421 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     3481 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     3541 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     3601 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     3661 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
     3721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4501 tacggttcct gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta
     4561 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
     4621 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
     4681 ttaatgtact ggggttgaac actctgtgcc gaattcggat ccggagagcg gagaattaag
     4741 ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga
     4801 cagaaccgca acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag
     4861 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
     4921 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat
     4981 tagagtctca tattcactct catactgtat ttttacaaca attaccaaca acaacaaaca
     5041 acaaacaaca ttacaattac tatttacaat tacaatgtct cctgaaagaa ggcctgctga
     5101 tattagaagg gctactgagg ctgatatgcc tgctgtgtgc actattgtga atcattacat
     5161 tgagacttct actgtgaatt tcaggactga gcctcaagaa ccacaagagt ggactgatga
     5221 tcttgtgagg cttagagaga ggtatccttg gcttgttgct gaagtggatg gtgaagtggc
     5281 tggtattgct tatgctggtc cttggaaggc taggaatgct tacgattgga ctgctgagtc
     5341 tactgtttac gtgtctccta ggcatcaaag aactggtctt ggatctactc tttacactca
     5401 tcttttgaag tctttggagg ctcagggttt caagtctgtg gttgctgtga ttggacttcc
     5461 taatgatcct tctgtgagga tgcatgaggc tcttggttat gctcctaggg gaatgcttag
     5521 agctgctggt ttcaaacatg gaaattggca tgatgtggga ttctggcagc ttgatttctc
     5581 tcttcctgtt cctcctagac ctgttcttcc tgtgactgag atttgagctt gtcctgcttt
     5641 aatgagatat gcgagaagcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
     5701 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
     5761 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
     5821 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
     5881 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
     5941 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
     6001 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
     6061 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
     6121 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
     6181 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
     6241 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     6301 gtccagggcg aattttgcga caacatgtcg aggctcagca ggaccgctgc aagaattcaa
     6361 gcttggagct caacttttga ttcgctattt gcagtgcacc tgtggcgttc atcacatctt
     6421 ttgtgacact gtttgcactg gtcattgcta ttacaaagga ccttcctgat gttgaaggag
     6481 atcgaaagta agtaactgca cgcataacca ttttctttcc gctctttggc tcaatccatt
     6541 tgacagtcaa agacaatgtt taaccagctc cgtttgatat attgtcttta tgtgtttgtt
     6601 caagcatgtt tagttaatca tgcctttgat tgatcttgaa taggttccaa atatcaaccc
     6661 tggcaacaaa acttggagtg agaaacattg cattcctcgg ttctggactt ctgctagtaa
     6721 attatgtttc agccatatca ctagctttct acatgcctca ggtgaattca tctatttccg
     6781 tcttaactat ttcggttaat taaagcacga acaccattac tgcatgtaga agcttgataa
     6841 actatcgcca ccaatttatt tttgttgcga tattgttact ttcctcagta tgcagctttg
     6901 aaaagaccaa ccctcttatc ctttaacaat gaacaggttt ttagaggtag cttgatgatt
     6961 cctgcacatg tgatcttggc ttcaggctta attttccagg taaagcatta tgagatactc
     7021 ttatatctct tacatacttt tgagataatg cacaagaact tcataactat atgctttagt
     7081 ttctgcattt gacactgcca aattcattaa tctctaatat ctttgttgtt gatctttggt
     7141 agacatgggt actagaaaaa gcaaactaca ccaaggtaaa atacttttgt acaaacataa
     7201 actcgttatc acggaacatc aatggagtgt atatctaacg gagtgtagaa acatttgatt
     7261 attgcaggaa gctatctcag gatattatcg gtttatatgg aatctcttct acgcagagta
     7321 tctgttattc cccttcctct agctttcaat ttcatggtga ggatatgcag ttttctttgt
     7381 atatcattct tcttcttctt tgtagcttgg agtcaaaatc ggttccttca tgtacataca
     7441 tcaaggatat gtccttctga atttttatat cttgcaataa aaatgcttgt accaattgaa
     7501 acaccagctt tttgagttct atgatcactg acttggttct aaccaaaaaa aaaaaaatgt
     7561 ttaatttaca tatctaaaag taggtttagg gaaacctaaa cagtaaaata tttgtatatt
     7621 attcgaattt cactcatcat aaaaacttaa attgcaccat aaaattttgt tttactatta
     7681 atgatgtaat ttgtgtaact taagataaaa ataatattcc gtaagttaac cggctaaaac
     7741 cacgtataaa ccagggaacc tgttaaaccg gttctttact ggataaagaa atgaaagccc
     7801 atgtagacag ctccattaga gcccaaaccc taaatttctc atctatataa aaggagtgac
     7861 attagggttt ttgttcgtcc tcttaaagct tctcgttttc tctgccgtct ctctcattcg
     7921 cgcgacgcaa acgatcttca ggtgatcttc tttctccaaa tcctctctca taactctgat
     7981 ttcgtacttg tgtatttgag ctcacgctct gtttctctca ccacagcaat ggcttctagc
     8041 ccaccgaaga agaagcggaa ggtcagctgg aaaatggaca agaagtacag cattggactt
     8101 gatattggta cgaactcagt tgggtgggcc gttatcaccg atgaatacaa ggtaccttcg
     8161 aagaaattta aagtgctggg caacacagat aggcacagca ttaagaagaa cttgatcgga
     8221 gctctgctct ttgactctgg agaaaccgcg gaggcgacaa ggcttaaacg tactgcgagg
     8281 agaaggtaca ctcgcaggaa gaacagaatc tgttatctcc aagagatctt tagcaacgag
     8341 atggcgaagg taaggatttt tatgatatag tatgcttatg tattttgtac tgaaagcata
     8401 tcctgcttca ttgggatatt actgaaagca tttaactaca tgtaaactca cttgatgatc
     8461 aataaacttg attttgcagg ttgacgactc gttcttccat cgcctcgagg aatccttcct
     8521 ggtagaggaa gataagaaac acgagcgtca ccccatcttt gggaatattg ttgacgaagt
     8581 agcctatcat gaaaagtatc cgactatata ccaccttcgc aagaagctgg tggactcaac
     8641 cgataaggca gaccttcggc tcatatacct ggctctcgcg cacatgataa agtttcgtgg
     8701 ccatttcttg atcgaagggg acctcaaccc ggataactcc gatgtggata aactgttcat
     8761 tcagctcgtc caaacctaca atcagctgtt cgaggagaac cccatcaatg catcaggtaa
     8821 cattccttag ttacctttct tttctttttc catcataagt ttatagattg tacatgcttt
     8881 gagatttttc tttgcaaaca atctcaggtg tcgacgccaa ggcaatactg tctgccagac
     8941 tttcgaagtc cagacggctt gagaatctga tcgctcaatt gccaggcgag aagaagaacg
     9001 gcttgttcgg gaatctgatt gcactgtctc tgggcctcac ccctaacttc aaaagcaact
     9061 ttgacctcgc cgaggacgcg aagctgcagc tgtcaaagga tacatacgat gatgatctgg
     9121 acaatctgct cgcccaaata ggtgctcttg aaattggaac tcttcttttg ttgtctaaac
     9181 ctatcaattt ctttgcggaa atttatttga agctgtagag ttaaaattga gtcttttaaa
     9241 cttttgtagg tgatcagtat gccgacctgt tcttggctgc caagaatctg tcagacgcta
     9301 tcttgctcag tgacattctg cgggtcaaca cggagataac caaagcgcca cttagcgcct
     9361 ccatgatcaa gaggtacgac gagcatcacc aggatctgac ccttctgaag gctttggttc
     9421 gccagcaact ccccgagaag tacaaggaga ttttctttga ccaatcgaag aatggctacg
     9481 cagggtacat tgatggaggt aagttgttac ttatgattgt tttcctctct gctacatgta
     9541 ttttgttgtt catttctgta agatataaga attgagtttt cctctgatga tattattagg
     9601 tgcaagtcag gaggaattct acaaattcat caagcctatt ctggaaaaga tggacggtac
     9661 agaggagctg ctcgttaaat tgaaccgcga agatttgctt cggaagcagc gtaccttcga
     9721 caatggcagc ataccgcacc agatccacct cggtgagctg catgctatct tgaggaggca
     9781 agaggacttc tatccgttcc tgaaagacaa cagagagaag attgaaaaga tcctcacgtt
     9841 ccgcattccc tactatgtag gttagtatca tatgaagaaa tacctagttt cagttgatga
     9901 atgctatttt ctgacctcag ttgttctctt ttgagaatta tttcttttct aatttgcctg
     9961 atttttctat taattcatta ggtccactcg cacgcgggaa ctcgcggttt gcgtggatga
    10021 cacgcaaatc cgaggagact atcacgcctt ggaacttcga agaggtcgtg gacaagggtg
    10081 cgagtgcaca gtccttcatc gaaaggatga ccaacttcga taagaatctc ccaaatgaga
    10141 aagtcctgcc caagcatagt ctcctgtacg aatacttcac ggtctacaac gagctgacga
    10201 aggtgaaata tgtgacggag gggatgcgca aaccggcctt cctgtcaggt aaatcctggt
    10261 ccacactttt acgataaaaa cacaagattt taaactatga actgatcaat aatcattcct
    10321 aaaagaccac acttttgttt tgtttctaaa gtaattttta ctgttataac aggtgagcag
    10381 aagaaggcca ttgtcgatct cttgttcaaa accaatcgga aggtcactgt gaaacagctt
    10441 aaagaggact actttaagaa gatcgaatgc tttgattctg tggaaatcag cggcgttgag
    10501 gataggttca atgcctctct tggcacatac catgacctgt tgaaaatcat caaggacaag
    10561 gacttccttg acaacgagga gaacgaggac atcctcgagg acatcgtgct gactctcacg
    10621 ctgtttgagg acagagaaat gatcgaggag cgccttaaga cttatgcgca tctgttcgat
    10681 gacaaggtca tgaagcagtt gaagaggagg agatatacag gtaagaggtc aaaaggtttc
    10741 cgcaatgatc cctctttttt tgtttctcta gtttcaagaa tttgggtata tgactaactt
    10801 ctgagtgttc cttgatgcat atttgtgatg agacaaatgt ttgttctatg ttttaggttg
    10861 gggaaggctc tccaggaagc tcatcaacgg catccgcgac aagcaatccg gcaagactat
    10921 actggacttt ctcaaatccg acggttttgc gaatcggaac ttcatgcagc ttattcacga
    10981 tgactcactg accttcaaag aagatatcca gaaggcccaa gtgtcaggtc agggcgatag
    11041 ccttcacgaa cacatagcca acctggctgg atcgccagct ataaagaagg gcatactgca
    11101 gacagtgaag gttgtggatg agctggtgaa ggtaagttct gcatttggtt atgctccttg
    11161 cattttaggt gttcgtcgca cttccatttc catgaatagc taagattttt tttctctgca
    11221 ttcattcttc ttgcctcagt tctaactgtt tgtggtattt ttgttttaat tattgctaca
    11281 ggtcatgggc cgccataagc cggagaacat cgtcatcgag atggcgaggg aaaaccagac
    11341 gactcagaaa gggcagaaga actcacggga gcgcatgaag cggatagagg aaggcatcaa
    11401 ggagcttggg agtcagattc tgaaagagca cccagtcgaa aatactcaac tccagaacga
    11461 gaagctgtac ctctattacc tccagaatgg gagagatatg tacgtcgacc aagagctcga
    11521 cattaacaga ctctccgact atgatgtgga tcacattgtc cctcaatctt tcctgaagga
    11581 cgatagtatt gacaacaagg taaagcaact gtgttttaat caatttcttg tcaggatata
    11641 tggattataa cttaattttt gagaaatctg tagtatttgg cgtgaaatga gtttgctttt
    11701 tggtttctcc cgtgttatag gtccttacgc gctcagacaa gaaccgcgga aaatccgaca
    11761 atgtacccag cgaggaggtt gtgaagaaga tgaagaacta ttggaggcag cttttgaatg
    11821 ctaagctcat aacccaacgg aaattcgaca atctcacgaa ggcagaaagg ggcggactgt
    11881 ctgagctcga caaagccggc ttcatcaagc gccagttggt tgaaactcgt cagattacga
    11941 aacatgtggc ccagatactc gattcgcgta tgaatacgaa gtatgatgag aatgacaaac
    12001 ttatcaggga ggtaaaggta aagtttccaa ctttccttta ccatatcaaa ctaaagttcg
    12061 aaacttttta tttgatcaac ttcaaggcca cccgatcttt ctattcctga ttaatttgtg
    12121 atgaatccat attgactttt gatggttacg caggtgatca ccctcaagag caaactggtt
    12181 agtgacttcc ggaaggactt ccagttttac aaggttcgcg agatcaacaa ctaccatcat
    12241 gcccatgacg cctacctgaa cgccgttgtt ggcactgctc tcatcaagaa gtatccgaaa
    12301 ctggagtctg agtttgtgta cggggattac aaggtgtacg acgttaggaa gatgatcgcg
    12361 aagtcagaac aagagatcgg caaggctacc gcgaaatact tcttttactc gaatatcatg
    12421 aacttcttca agacagagat cactctggcg aatggtgaaa tccggaagag gcctctgatc
    12481 gagacaaatg gcgaaacagg tctgtctttc ctatttcata tgtttaatcc taggaatttg
    12541 atcaattgat tgtatgtatg tcgatcccaa gactttcttg ttcacttata tcttaactct
    12601 ctctttgctg tttcttgcag gtgagattgt ctgggataag ggcagggatt ttgcgactgt
    12661 gcgtaaggtt ctcagcatgc cccaagtcaa catagtcaag aaaacggagg ttcaaaccgg
    12721 tggtttctcc aaggagtcca ttctccctaa gcgcaactcc gacaaactga ttgcgaggaa
    12781 gaaggattgg gatccgaaga aatacggagg ctttgatagc cctaccgtgg catacagcgt
    12841 actggtagtg gccaaggtgg agaagggcaa gagcaagaaa ctgaaaagcg tcaaggaact
    12901 gcttggaatt accataatgg aaaggtcctc gttcgagaag aatccgatcg acttcctcga
    12961 ggctaaaggt aaaatattgg atgccagacg atattctttc ttttgatttg taactttttc
    13021 ctgtcaaggt cgataaattt tatttttttt ggtaaaaggt cgataatttt tttttggagc
    13081 cattatgtaa ttttcctaat taactgaacc aaaattatac aaaccaggtt acaaagaggt
    13141 gaagaaagac ctcattatca aactgcccaa gtattcgctt ttcgaattgg aaaatggcag
    13201 aaaacgcatg ctggcatctg ccggagaact gcagaagggc aacgagctgg cattgcccag
    13261 taagtacgtc aacttcctgt acttggcctc acactatgag aagctgaagg ggtcaccaga
    13321 ggacaacgag cagaagcagt tgtttgtcga gcagcacaag cactatcttg atgagatcat
    13381 agagcagatc agcgaatttt ccaagcgggt cattcttgca gacgctaacc tcgataaggt
    13441 aaggacttct catgaatatt agtggcagat tagtgttgtt aaagtctttg gttagataat
    13501 cgatgcctcc taattgtcca tgttttactg gttttctaca attaaaggtg ctttccgcgt
    13561 acaacaagca cagagataag ccgataaggg aacaagcgga aaacatcatc cacctgttca
    13621 cactgaccaa tctgggagcc ccagcagcct ttaagtactt cgataccact atcgacagaa
    13681 agcgctacac atcaaccaag gaagtgttgg acgctaccct tattcaccaa tctattacag
    13741 ggctctatga gacaaggata gatctgtcgc agttgggtgg tgactctagg gctgacccaa
    13801 agaagaagcg taaagtctga gcttgatcct ctagagtcaa gcagatcgtt caaacatttg
    13861 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
    13921 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
    13981 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
    14041 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg
    14101 ctactattgt cttcgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    14161 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    14221 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    14281 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
    14341 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    14401 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    14461 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
    14521 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    14581 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    14641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acgaagacaa
//
