LOCUS       sequence-307959-       14469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 cloning vector for one or several guide RNAs, already 
            contains Cas9 and a kanamycin selection cassette for plant 
            transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT,
            Stuttmann J, Marillonnet S
  TITLE     High-efficiency genome editing in plants mediated by a Cas9 gene 
            containing multiple introns.
  JOURNAL   Plant Commun. 2020 Nov 23;2(2):100135. doi: 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8.
  PUBMED    33898975
REFERENCE   2  (bases 1 to 14469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2020-11-23"; volume: "2(2):100135. doi"; pages: " 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8"
COMMENT     Sequence Label: pAGM65881
FEATURES             Location/Qualifiers
     source          1..14469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             367..996
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1425..2498
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2820..2924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2925..3719
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3890..4478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4379..4398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4614..4638
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4803..4982
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4835..5018
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5039..5833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5093..5112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5703..5722
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5859..6111
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             7813..7833
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13566..13586
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      13613..13865
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    13939..13960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13975..13980,13981..13998,13999..14005)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14013..14029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14018..14040
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14037..14053
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14037..14053
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             14049..14372
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    14066..14122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(14123..14140)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14123..14139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14132..14154)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(14348..14367)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgccgtgcg
      181 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
      241 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
      301 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
      361 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
      421 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
      481 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
      541 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
      601 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
      661 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
      721 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
      781 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
      841 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
      901 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
      961 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     1021 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     1081 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     1141 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     1201 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     1261 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     1321 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     1381 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     1441 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     1501 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     1561 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgtcgt
     1621 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     1681 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     1741 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     1801 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     1861 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     1921 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     1981 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     2041 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     2101 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     2161 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     2221 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     2281 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     2341 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     2401 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     2461 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     2521 aaattgccct agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat agcacgtaca
     2581 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     2641 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     2701 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     2761 tgtctggcca gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg ctccgagtgc
     2821 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2881 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
     2941 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     3001 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     3061 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     3121 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     3181 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     3241 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     3301 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     3361 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
     3421 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     3481 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     3541 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     3601 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     3661 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
     3721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4501 tacggttcct gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta
     4561 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
     4621 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
     4681 ttaatgtact ggggttgaac actctgtgcc gaattcggat ccggagagcg gagaattaag
     4741 ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga
     4801 cagaaccgca acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag
     4861 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
     4921 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat
     4981 tagagtctca tattcactct caatccaaat aatctgcacc ggatctggat cgtttcgcat
     5041 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5101 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5161 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
     5221 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5281 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5341 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5401 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5461 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5521 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgta tgcccgacgg
     5581 cgaggatctc gtcgtgactc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5641 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5701 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5761 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5821 cgagttcttc tgagcgggtc tggatctaga gtcaagcaga tcgttcaaac atttggcaat
     5881 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     5941 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     6001 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     6061 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgacgctgca
     6121 agaattcaag cttggagctc aacttttgat tcgctatttg cagtgcacct gtggcgttca
     6181 tcacatcttt tgtgacactg tttgcactgg tcattgctat tacaaaggac cttcctgatg
     6241 ttgaaggaga tcgaaagtaa gtaactgcac gcataaccat tttctttccg ctctttggct
     6301 caatccattt gacagtcaaa gacaatgttt aaccagctcc gtttgatata ttgtctttat
     6361 gtgtttgttc aagcatgttt agttaatcat gcctttgatt gatcttgaat aggttccaaa
     6421 tatcaaccct ggcaacaaaa cttggagtga gaaacattgc attcctcggt tctggacttc
     6481 tgctagtaaa ttatgtttca gccatatcac tagctttcta catgcctcag gtgaattcat
     6541 ctatttccgt cttaactatt tcggttaatt aaagcacgaa caccattact gcatgtagaa
     6601 gcttgataaa ctatcgccac caatttattt ttgttgcgat attgttactt tcctcagtat
     6661 gcagctttga aaagaccaac cctcttatcc tttaacaatg aacaggtttt tagaggtagc
     6721 ttgatgattc ctgcacatgt gatcttggct tcaggcttaa ttttccaggt aaagcattat
     6781 gagatactct tatatctctt acatactttt gagataatgc acaagaactt cataactata
     6841 tgctttagtt tctgcatttg acactgccaa attcattaat ctctaatatc tttgttgttg
     6901 atctttggta gacatgggta ctagaaaaag caaactacac caaggtaaaa tacttttgta
     6961 caaacataaa ctcgttatca cggaacatca atggagtgta tatctaacgg agtgtagaaa
     7021 catttgatta ttgcaggaag ctatctcagg atattatcgg tttatatgga atctcttcta
     7081 cgcagagtat ctgttattcc ccttcctcta gctttcaatt tcatggtgag gatatgcagt
     7141 tttctttgta tatcattctt cttcttcttt gtagcttgga gtcaaaatcg gttccttcat
     7201 gtacatacat caaggatatg tccttctgaa tttttatatc ttgcaataaa aatgcttgta
     7261 ccaattgaaa caccagcttt ttgagttcta tgatcactga cttggttcta accaaaaaaa
     7321 aaaaaatgtt taatttacat atctaaaagt aggtttaggg aaacctaaac agtaaaatat
     7381 ttgtatatta ttcgaatttc actcatcata aaaacttaaa ttgcaccata aaattttgtt
     7441 ttactattaa tgatgtaatt tgtgtaactt aagataaaaa taatattccg taagttaacc
     7501 ggctaaaacc acgtataaac cagggaacct gttaaaccgg ttctttactg gataaagaaa
     7561 tgaaagccca tgtagacagc tccattagag cccaaaccct aaatttctca tctatataaa
     7621 aggagtgaca ttagggtttt tgttcgtcct cttaaagctt ctcgttttct ctgccgtctc
     7681 tctcattcgc gcgacgcaaa cgatcttcag gtgatcttct ttctccaaat cctctctcat
     7741 aactctgatt tcgtacttgt gtatttgagc tcacgctctg tttctctcac cacagcaatg
     7801 gcttctagcc caccgaagaa gaagcggaag gtcagctgga aaatggacaa gaagtacagc
     7861 attggacttg atattggtac gaactcagtt gggtgggccg ttatcaccga tgaatacaag
     7921 gtaccttcga agaaatttaa agtgctgggc aacacagata ggcacagcat taagaagaac
     7981 ttgatcggag ctctgctctt tgactctgga gaaaccgcgg aggcgacaag gcttaaacgt
     8041 actgcgagga gaaggtacac tcgcaggaag aacagaatct gttatctcca agagatcttt
     8101 agcaacgaga tggcgaaggt aaggattttt atgatatagt atgcttatgt attttgtact
     8161 gaaagcatat cctgcttcat tgggatatta ctgaaagcat ttaactacat gtaaactcac
     8221 ttgatgatca ataaacttga ttttgcaggt tgacgactcg ttcttccatc gcctcgagga
     8281 atccttcctg gtagaggaag ataagaaaca cgagcgtcac cccatctttg ggaatattgt
     8341 tgacgaagta gcctatcatg aaaagtatcc gactatatac caccttcgca agaagctggt
     8401 ggactcaacc gataaggcag accttcggct catatacctg gctctcgcgc acatgataaa
     8461 gtttcgtggc catttcttga tcgaagggga cctcaacccg gataactccg atgtggataa
     8521 actgttcatt cagctcgtcc aaacctacaa tcagctgttc gaggagaacc ccatcaatgc
     8581 atcaggtaac attccttagt tacctttctt ttctttttcc atcataagtt tatagattgt
     8641 acatgctttg agatttttct ttgcaaacaa tctcaggtgt cgacgccaag gcaatactgt
     8701 ctgccagact ttcgaagtcc agacggcttg agaatctgat cgctcaattg ccaggcgaga
     8761 agaagaacgg cttgttcggg aatctgattg cactgtctct gggcctcacc cctaacttca
     8821 aaagcaactt tgacctcgcc gaggacgcga agctgcagct gtcaaaggat acatacgatg
     8881 atgatctgga caatctgctc gcccaaatag gtgctcttga aattggaact cttcttttgt
     8941 tgtctaaacc tatcaatttc tttgcggaaa tttatttgaa gctgtagagt taaaattgag
     9001 tcttttaaac ttttgtaggt gatcagtatg ccgacctgtt cttggctgcc aagaatctgt
     9061 cagacgctat cttgctcagt gacattctgc gggtcaacac ggagataacc aaagcgccac
     9121 ttagcgcctc catgatcaag aggtacgacg agcatcacca ggatctgacc cttctgaagg
     9181 ctttggttcg ccagcaactc cccgagaagt acaaggagat tttctttgac caatcgaaga
     9241 atggctacgc agggtacatt gatggaggta agttgttact tatgattgtt ttcctctctg
     9301 ctacatgtat tttgttgttc atttctgtaa gatataagaa ttgagttttc ctctgatgat
     9361 attattaggt gcaagtcagg aggaattcta caaattcatc aagcctattc tggaaaagat
     9421 ggacggtaca gaggagctgc tcgttaaatt gaaccgcgaa gatttgcttc ggaagcagcg
     9481 taccttcgac aatggcagca taccgcacca gatccacctc ggtgagctgc atgctatctt
     9541 gaggaggcaa gaggacttct atccgttcct gaaagacaac agagagaaga ttgaaaagat
     9601 cctcacgttc cgcattccct actatgtagg ttagtatcat atgaagaaat acctagtttc
     9661 agttgatgaa tgctattttc tgacctcagt tgttctcttt tgagaattat ttcttttcta
     9721 atttgcctga tttttctatt aattcattag gtccactcgc acgcgggaac tcgcggtttg
     9781 cgtggatgac acgcaaatcc gaggagacta tcacgccttg gaacttcgaa gaggtcgtgg
     9841 acaagggtgc gagtgcacag tccttcatcg aaaggatgac caacttcgat aagaatctcc
     9901 caaatgagaa agtcctgccc aagcatagtc tcctgtacga atacttcacg gtctacaacg
     9961 agctgacgaa ggtgaaatat gtgacggagg ggatgcgcaa accggccttc ctgtcaggta
    10021 aatcctggtc cacactttta cgataaaaac acaagatttt aaactatgaa ctgatcaata
    10081 atcattccta aaagaccaca cttttgtttt gtttctaaag taatttttac tgttataaca
    10141 ggtgagcaga agaaggccat tgtcgatctc ttgttcaaaa ccaatcggaa ggtcactgtg
    10201 aaacagctta aagaggacta ctttaagaag atcgaatgct ttgattctgt ggaaatcagc
    10261 ggcgttgagg ataggttcaa tgcctctctt ggcacatacc atgacctgtt gaaaatcatc
    10321 aaggacaagg acttccttga caacgaggag aacgaggaca tcctcgagga catcgtgctg
    10381 actctcacgc tgtttgagga cagagaaatg atcgaggagc gccttaagac ttatgcgcat
    10441 ctgttcgatg acaaggtcat gaagcagttg aagaggagga gatatacagg taagaggtca
    10501 aaaggtttcc gcaatgatcc ctcttttttt gtttctctag tttcaagaat ttgggtatat
    10561 gactaacttc tgagtgttcc ttgatgcata tttgtgatga gacaaatgtt tgttctatgt
    10621 tttaggttgg ggaaggctct ccaggaagct catcaacggc atccgcgaca agcaatccgg
    10681 caagactata ctggactttc tcaaatccga cggttttgcg aatcggaact tcatgcagct
    10741 tattcacgat gactcactga ccttcaaaga agatatccag aaggcccaag tgtcaggtca
    10801 gggcgatagc cttcacgaac acatagccaa cctggctgga tcgccagcta taaagaaggg
    10861 catactgcag acagtgaagg ttgtggatga gctggtgaag gtaagttctg catttggtta
    10921 tgctccttgc attttaggtg ttcgtcgcac ttccatttcc atgaatagct aagatttttt
    10981 ttctctgcat tcattcttct tgcctcagtt ctaactgttt gtggtatttt tgttttaatt
    11041 attgctacag gtcatgggcc gccataagcc ggagaacatc gtcatcgaga tggcgaggga
    11101 aaaccagacg actcagaaag ggcagaagaa ctcacgggag cgcatgaagc ggatagagga
    11161 aggcatcaag gagcttggga gtcagattct gaaagagcac ccagtcgaaa atactcaact
    11221 ccagaacgag aagctgtacc tctattacct ccagaatggg agagatatgt acgtcgacca
    11281 agagctcgac attaacagac tctccgacta tgatgtggat cacattgtcc ctcaatcttt
    11341 cctgaaggac gatagtattg acaacaaggt aaagcaactg tgttttaatc aatttcttgt
    11401 caggatatat ggattataac ttaatttttg agaaatctgt agtatttggc gtgaaatgag
    11461 tttgcttttt ggtttctccc gtgttatagg tccttacgcg ctcagacaag aaccgcggaa
    11521 aatccgacaa tgtacccagc gaggaggttg tgaagaagat gaagaactat tggaggcagc
    11581 ttttgaatgc taagctcata acccaacgga aattcgacaa tctcacgaag gcagaaaggg
    11641 gcggactgtc tgagctcgac aaagccggct tcatcaagcg ccagttggtt gaaactcgtc
    11701 agattacgaa acatgtggcc cagatactcg attcgcgtat gaatacgaag tatgatgaga
    11761 atgacaaact tatcagggag gtaaaggtaa agtttccaac tttcctttac catatcaaac
    11821 taaagttcga aactttttat ttgatcaact tcaaggccac ccgatctttc tattcctgat
    11881 taatttgtga tgaatccata ttgacttttg atggttacgc aggtgatcac cctcaagagc
    11941 aaactggtta gtgacttccg gaaggacttc cagttttaca aggttcgcga gatcaacaac
    12001 taccatcatg cccatgacgc ctacctgaac gccgttgttg gcactgctct catcaagaag
    12061 tatccgaaac tggagtctga gtttgtgtac ggggattaca aggtgtacga cgttaggaag
    12121 atgatcgcga agtcagaaca agagatcggc aaggctaccg cgaaatactt cttttactcg
    12181 aatatcatga acttcttcaa gacagagatc actctggcga atggtgaaat ccggaagagg
    12241 cctctgatcg agacaaatgg cgaaacaggt ctgtctttcc tatttcatat gtttaatcct
    12301 aggaatttga tcaattgatt gtatgtatgt cgatcccaag actttcttgt tcacttatat
    12361 cttaactctc tctttgctgt ttcttgcagg tgagattgtc tgggataagg gcagggattt
    12421 tgcgactgtg cgtaaggttc tcagcatgcc ccaagtcaac atagtcaaga aaacggaggt
    12481 tcaaaccggt ggtttctcca aggagtccat tctccctaag cgcaactccg acaaactgat
    12541 tgcgaggaag aaggattggg atccgaagaa atacggaggc tttgatagcc ctaccgtggc
    12601 atacagcgta ctggtagtgg ccaaggtgga gaagggcaag agcaagaaac tgaaaagcgt
    12661 caaggaactg cttggaatta ccataatgga aaggtcctcg ttcgagaaga atccgatcga
    12721 cttcctcgag gctaaaggta aaatattgga tgccagacga tattctttct tttgatttgt
    12781 aactttttcc tgtcaaggtc gataaatttt attttttttg gtaaaaggtc gataattttt
    12841 ttttggagcc attatgtaat tttcctaatt aactgaacca aaattataca aaccaggtta
    12901 caaagaggtg aagaaagacc tcattatcaa actgcccaag tattcgcttt tcgaattgga
    12961 aaatggcaga aaacgcatgc tggcatctgc cggagaactg cagaagggca acgagctggc
    13021 attgcccagt aagtacgtca acttcctgta cttggcctca cactatgaga agctgaaggg
    13081 gtcaccagag gacaacgagc agaagcagtt gtttgtcgag cagcacaagc actatcttga
    13141 tgagatcata gagcagatca gcgaattttc caagcgggtc attcttgcag acgctaacct
    13201 cgataaggta aggacttctc atgaatatta gtggcagatt agtgttgtta aagtctttgg
    13261 ttagataatc gatgcctcct aattgtccat gttttactgg ttttctacaa ttaaaggtgc
    13321 tttccgcgta caacaagcac agagataagc cgataaggga acaagcggaa aacatcatcc
    13381 acctgttcac actgaccaat ctgggagccc cagcagcctt taagtacttc gataccacta
    13441 tcgacagaaa gcgctacaca tcaaccaagg aagtgttgga cgctaccctt attcaccaat
    13501 ctattacagg gctctatgag acaaggatag atctgtcgca gttgggtggt gactctaggg
    13561 ctgacccaaa gaagaagcgt aaagtctgag cttgatcctc tagagtcaag cagatcgttc
    13621 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
    13681 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
    13741 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
    13801 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
    13861 agatcgacgc tactattgtc ttcgcagctg gcacgacagg tttcccgact ggaaagcggg
    13921 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    13981 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    14041 aaacagctat gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc
    14101 cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    14161 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    14221 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
    14281 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
    14341 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
    14401 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    14461 cgaagacaa
//
