LOCUS       sequence-307972-       11500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in 
            Schizosaccharomyces pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     CRISPR-Cas12a system in fission yeast for multiplex genomic editing 
            and CRISPR interference.
  JOURNAL   Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 
            10.1093/nar/gkaa329.
  PUBMED    32374858
REFERENCE   2  (bases 1 to 11500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-06-4- 4"; volume: "48"; issue: 
            "10"; pages: "5788-5798"
COMMENT     Sequence Label: pYZ221
FEATURES             Location/Qualifiers
     source          1..11500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        325..681
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     misc_RNA        709..760
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(829..845)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(830..846)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(870..889)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(871..889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(899..916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(899..915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(908..930)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1056..1511
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1143..1162)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1353..1374
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1947..2741)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      3361..4144
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        4330..5071
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             8992..9039
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9046..9126
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      9142..9389
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(9158..9176)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        9595..9699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9700..9768,9769..10560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9918..9937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10731..11319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11220..11239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11473..11490
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctcgg atccactagt aacggccgcc
      301 agtgtgctgg aattcgccct tccttttgct tatgttggtg gtagttggca tgcgtagact
      361 gatgactagt cagcaaggag cgtagaacag tcacactcgt tatatatgtg cttccaagaa
      421 aactcaagaa tttaccatta gcaaacactt ttttgaaatg ttagacattt aaatgacgaa
      481 ggcatataga agctttgaat aggtgttgta aagtgttgat ttatgtgacg ctgagggtgc
      541 gcatgaaagg aatgttgggt cacgattatt aaacagtttg ctagcttgga cacttgagta
      601 ttggaagttg ttgaattcta aaaaactttc agttgatttg aatagttgct gttgccaaaa
      661 aacataacct gtaccgaaga aaatttctac tgttgtagat gcggccgccc tgtcaccgga
      721 tgtgctttcc ggtctgatga gtccgtgagg acgaaacagg ggtaccgtgg ggatcctcta
      781 gagtcgacct gaagggcgaa ttctgcagga attcgatatc aagcttatcg ataccgtcga
      841 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      901 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      961 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1021 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     1081 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1141 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1201 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1261 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1321 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1381 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     1441 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     1501 gtttacaatt tagcttgtga tattgacgaa actttttgac atctaattta ttctgttcca
     1561 acaccaatgt ttataaccaa gttttatctt gtttgtctac atggtatttt acattcatct
     1621 acatacatct ttcattggct ttgtacatag ttatcattac aagtctaaaa aaattcactc
     1681 ttttcttatt caatgtcaat ccaagagaaa agattgtggt aatgttgtag gagcatgttt
     1741 aataaattac tatagcaaat tactttttat tcccaaggtg tttatctata atagttaata
     1801 ttttagtcgc tacataaaat tttaccaaag agtacttgta tactaattct aaatgccttc
     1861 tgacataaaa cgcctaggaa aacaaacgca aacaaggcat cgactttttc aataaccaac
     1921 caaaaaaatt ttacattagt ctttttttaa tgctgagaaa gtctttgctg atatgccttc
     1981 caaccagctt ctctatatct cttggcttcg acaacaggat tacgaccagc tccatagact
     2041 ccacgaccaa caatgatgat atcgctaccg cagtttacaa tcacttcttc aggagtacga
     2101 tattgctgtc ccagcccgtc tcctttaaca tccaagccga taccagggga catagttatg
     2161 tagtcgcttt gaaggttagg aaatcgacga ccagctataa agccaaagca aaaatcggta
     2221 tgcttctcaa accattctaa ggttttctct gtgtaggaac cagtagccaa agagcctttg
     2281 gaagacattt cagccaaaag caagagtcca cgtcccaaag gtaaaccaac ttctttgagg
     2341 ccttgtataa taccctcgcc tggcactgta tggcaatttg tgatatgagc ccaagaagca
     2401 attttgtaca caccagatgc atattgtagc ttgacggtat ttccaatgtc tgcgaatttg
     2461 cgatcctcaa agataagaaa acgatgcttt ttacctaagg ccaccagttt ttctaccata
     2521 tcctggtcga aatcctcgac aacgtcaata tgtgtcttga taacacagac atagggtcca
     2581 attttatcta ccaattctaa gatttcggat ttcttcgtca aatcgaccgc gactgacaag
     2641 ttgctttgct tttcttccat caaagccaac aattccttgg caatgggatt tttcatcccc
     2701 tcagctctag ctgaatagct ttgaaatact ctagcatcca taactttgct tttaaacctt
     2761 taatttcgat ccaagcaaaa aagaggttct tggtaggaca atacggtaag aaaacacgac
     2821 atgtgcagag atgccgacga agcatagtta aactgggatg gtaaaatcaa ttaagaattt
     2881 ataaagacaa aattgtataa gtctctaaaa catcttaatt atacctcaca gaactatcta
     2941 aaatatattc acaaagtgca aacattatca tgaaaaagaa ccattttaat ttaaagcaag
     3001 ggcattaagg cttatttaca gaatttctta cttttgtaaa gattataagg ctgattatct
     3061 ttttcaccat gccaaaaatt acacaagata gaatggatgt ttgaaattaa acgtgagtat
     3121 acaaacaaat acactaggta aatcgaaaca tttttttctc cattaagtaa caaattccta
     3181 tttagagaaa gaatgctgag tagattaaat aatctataca aactttttta acacaaatgc
     3241 atacatatag ccagtgggat ttgtagctag tcgatcgact ctagaggaaa ttcgagtcta
     3301 actccttaac cactcggaca tagtgactta tctgacactc attgtaaatt aatatatata
     3361 taggcatttt gtttagttaa aggtacttaa gtaattagta taaacgaacc aattttataa
     3421 tcaggaagtt aagtgaatgg tagcacatgt cgtaaaaatt gtgaattttt attgaataat
     3481 attttaaata caagcctttc tagactaggt atactcataa acatatatga gcaaaaggat
     3541 agaggagatt acattgcatc ttctacaaat ttatttattg ccctttactg aaaaattaaa
     3601 taatgagtac taaatgataa aaagcgctca gtacaagaaa gattgcaaaa atattgcatt
     3661 cttcatgaat taaagttgca tataaggcat attgaaagta atagtactaa aacagcagtt
     3721 agcgaaaatt aatagaatta tattcgcaag acaattgtga cattaaatta aaaaattgta
     3781 gaatttttac tatcctcttt aacgccatga gcctttataa aaaggttaaa ttagttttaa
     3841 cattcttttt ttgagtaaga gtttacaatt tatcaaaacc tgtgttatta tattcattag
     3901 tttcaattta ttagcatcta gagaaaaatc aattggcagt tacattgttg gaatttatga
     3961 agaaaaagaa tctacaacgg agaataagtt gctgatcgct ttccctaaaa ttgtatattt
     4021 tgctgagctt attttgacat ttcgttgaag ttttctctaa ttcgcattca ttttaagtaa
     4081 aacaatgaga aataaaatta caaaaaatac aaattaaaat acaattttta gctataatta
     4141 tagacgatgc ccttgtatcc cattctgtct cgcttgcccc tactttttat cttttatata
     4201 ccataatgaa cgctgccgct actaaccata ccccgatttt acatttcgga ctcccaagga
     4261 cgtacaaaat agaaaactat agaaaaaaat aatcagaaaa tagcatgtca tctctttgta
     4321 aaacgcagtg ccctacaaca actaagaaaa tggctatcat gcggaagctg gtgagaagaa
     4381 cagcatcggg acaagggaag gaagaacaaa gacaaagaaa acaaaagaaa gcaattgaaa
     4441 acaaaacaaa acaattttca ttccttctct tatcattcct tttcttttct tttctctcat
     4501 tcaacgcact ccatcgtatc cgtattcctc ttattttttc tctttctcta tatccatttc
     4561 tttctctcta ggtgtgtcct ctctctctct tcaatttctc tactccgcat tccaacgcat
     4621 ccttccccca acctcccatt tcctccttac ggcccgatag cgatcgtctt tccctcgcta
     4681 tcactcgcta ccggcccctc ctctgcaccg taacctccta cgtatttacc atatcataaa
     4741 gttttttccg acgcttatcg ctgaccccct gtcgccctcc tattggcttc cggattatct
     4801 tcttgtccat aaggtgatcc atgcttcctg aagattcccg aaatgtgtcc actttggcgg
     4861 ggaatcattc catccacttc tttctctctc gctttcctca ttcggcgctc cccttccgcg
     4921 tctcattggt cttccgctcc gtttttgctt tgccgatgtt acttggggag aggtgcgata
     4981 atcctttcgc aaaaactcgg tttgacgcct cccatggtat aaatagtggg tggtggacag
     5041 gtgccttcgc ttttctttaa gcaagagaat tgctgcaggt cgactctaga gatgagcatc
     5101 taccaggagt tcgtcaacaa gtattcactg agtaagacac tgcggttcga gctgatccca
     5161 cagggcaaga cactggagaa catcaaggcc cgaggcctga ttctggacga tgagaagcgg
     5221 gcaaaagact ataagaaagc caagcagatc attgataaat accaccagtt ctttatcgag
     5281 gaaattctga gctccgtgtg catcagtgag gatctgctgc agaattactc agacgtgtac
     5341 ttcaagctga agaagagcga cgatgacaac ctgcagaagg acttcaagtc cgccaaggac
     5401 accatcaaga aacagattag cgagtacatc aaggactccg aaaagtttaa aaatctgttc
     5461 aaccagaatc tgatcgatgc taagaaaggc caggagtccg acctgatcct gtggctgaaa
     5521 cagtctaagg acaatgggat tgaactgttc aaggctaact ccgatatcac tgatattgac
     5581 gaggcactgg aaatcatcaa gagcttcaag ggatggacca catactttaa aggcttccac
     5641 gagaaccgca agaacgtgta ctccagcaac gacattccta cctccatcat ctaccgaatc
     5701 gtcgatgaca atctgccaaa gttcctggag aacaaggcca aatatgaatc tctgaaggac
     5761 aaagctcccg aggcaattaa ttacgaacag atcaagaaag atctggctga ggaactgaca
     5821 ttcgatatcg actataagac tagcgaggtg aaccagaggg tcttttccct ggacgaggtg
     5881 tttgaaatcg ccaatttcaa caattacctg aaccagtccg gcattactaa attcaatacc
     5941 atcattggcg ggaagtttgt gaacggggag aataccaagc gcaagggaat taacgaatac
     6001 atcaatctgt atagccagca gatcaacgac aaaactctga agaaatacaa gatgtctgtg
     6061 ctgttcaaac agatcctgag tgataccgag tccaagtctt ttgtcattga taaactggaa
     6121 gatgactcag acgtggtcac taccatgcag agcttttatg agcagatcgc cgctttcaag
     6181 acagtggagg aaaaatctat taaggaaact ctgagtctgc tgttcgatga cctgaaagcc
     6241 cagaagctgg acctgagtaa gatctacttc aaaaacgata agagtctgac agacctgtca
     6301 cagcaggtgt ttgatgacta ttccgtgatt gggaccgccg tcctggagta cattacacag
     6361 cagatcgctc caaagaacct ggataatccc tctaagaaag agcaggaact gatcgctaag
     6421 aaaaccgaga aggcaaaata tctgagtctg gaaacaatta agctggcact ggaggagttc
     6481 aacaagcaca gggatattga caaacagtgc cgctttgagg aaatcctggc caacttcgca
     6541 gccatcccca tgatttttga tgagatcgcc cagaacaaag acaatctggc tcagatcagt
     6601 attaagtacc agaaccaggg caagaaagac ctgctgcagg cttcagcaga agatgacgtg
     6661 aaagccatca aggatctgct ggaccagacc aacaatctgc tgcacaagct gaaaatcttc
     6721 catattagtc agtcagagga taaggctaat atcctggata aagacgaaca cttctacctg
     6781 gtgttcgagg aatgttactt cgagctggca aacattgtcc ccctgtataa caagattagg
     6841 aactacatca cacagaagcc ttactctgac gagaagttta aactgaactt cgaaaatagt
     6901 accctggcca acgggtggga taagaacaag gagcctgaca acacagctat cctgttcatc
     6961 aaggatgaca agtactatct gggagtgatg aataagaaaa acaataagat cttcgatgac
     7021 aaagccatta aggagaacaa aggggaagga tacaagaaaa tcgtgtataa gctgctgccc
     7081 ggcgcaaata agatgctgcc taaggtgttc ttcagcgcca agagtatcaa attctacaac
     7141 ccatccgagg acatcctgcg gattagaaat cactcaacac atactaagaa cgggagcccc
     7201 cagaagggat atgagaaatt tgagttcaac atcgaggatt gcaggaagtt tattgacttc
     7261 tacaagcaga gcatctccaa acaccctgaa tggaaggatt ttggcttccg gttttccgac
     7321 acacagagat ataactctat cgacgagttc taccgcgagg tggaaaatca ggggtataag
     7381 ctgacttttg agaacatttc tgaaagttac atcgacagcg tggtcaatca gggaaagctg
     7441 tacctgttcc agatctataa caaagatttt tcagcataca gcaagggcag accaaacctg
     7501 catacactgt actggaaggc cctgttcgat gagaggaatc tgcaggacgt ggtctataaa
     7561 ctgaacggag aggccgaact gttttaccgg aagcagtcta ttcctaagaa aatcactcac
     7621 ccagctaagg aggccatcgc taacaagaac aaggacaatc ctaagaaaga gagcgtgttc
     7681 gaatacgatc tgattaagga caagcggttc accgaagata agttcttttt ccattgtcca
     7741 atcaccatta acttcaagtc aagcggcgct aacaagttca acgacgagat caatctgctg
     7801 ctgaaggaaa aagcaaacga tgtgcacatc ctgagcattg accgaggaga gcggcatctg
     7861 gcctactata ccctggtgga tggcaaaggg aatatcatta agcaggatac attcaacatc
     7921 attggcaatg accggatgaa aaccaactac cacgataaac tggctgcaat cgagaaggat
     7981 agagactcag ctaggaagga ctggaagaaa atcaacaaca ttaaggagat gaaggaaggc
     8041 tatctgagcc aggtggtcca tgagattgca aagctggtca tcgaatacaa tgccattgtg
     8101 gtgttcgagg atctgaactt cggctttaag agggggcgct ttaaggtgga aaaacaggtc
     8161 tatcagaagc tggagaaaat gctgatcgaa aagctgaatt acctggtgtt taaagataac
     8221 gagttcgaca agaccggagg cgtcctgaga gcctaccagc tgacagctcc ctttgaaact
     8281 ttcaagaaaa tgggaaaaca gacaggcatc atctactatg tgccagccgg attcacttcc
     8341 aagatctgcc ccgtgaccgg ctttgtcaac cagctgtacc ctaaatatga gtcagtgagc
     8401 aagtcccagg aatttttcag caagttcgat aagatctgtt ataatctgga caaggggtac
     8461 ttcgagtttt ccttcgatta caagaacttc ggcgacaagg ccgctaaggg gaaatggacc
     8521 attgcctcct tcggatctcg cctgatcaac tttcgaaatt ccgataaaaa ccacaattgg
     8581 gacactaggg aggtgtaccc aaccaaggag ctggaaaagc tgctgaaaga ctactctatc
     8641 gagtatggac atggcgaatg catcaaggca gccatctgtg gcgagagtga taagaaattt
     8701 ttcgccaagc tgacctcagt gctgaataca atcctgcaga tgcggaactc aaagaccggg
     8761 acagaactgg actatctgat tagccccgtg gctgatgtca acggaaactt cttcgacagc
     8821 agacaggcac ccaaaaatat gcctcaggat gcagacgcca acggggccta ccacatcggg
     8881 ctgaagggac tgatgctgct gggccggatc aagaacaatc aggaggggaa gaagctgaac
     8941 ctggtcatta agaacgagga atacttcgag tttgtccaga atagaaataa caaaaggccg
     9001 gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gatcctaccc atacgatgtt
     9061 ccagattacg cttatcccta cgacgtgcct gattatgcat acccatatga tgtccccgac
     9121 tatgcctaat ctcttctcga gtcatgtaat tagttatgtc acgcttacat tcacgccctc
     9181 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     9241 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     9301 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     9361 ttttgggacg ctcgaaggct ttaatttgcg atcccctttt cgtttgcaag aagaaaggaa
     9421 acaatggaga agagatcatc catttgtatg taaattttag taaacttgaa gaaatcacta
     9481 acaacttctc ttacttaggg attctatgca aaaccttgta aatcatctga tggaggactc
     9541 gatttaatga attggaaggt tggatttcca ggtggcactt ttcggggaaa tgtgcgcgga
     9601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10381 tttattgctg ataaatctgg agccggtgag cgtggatctc gcggtatcat tgcagcactg
    10441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10861 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11341 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11401 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11461 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
