LOCUS       sequence-307974-       13329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 
            in Schizosaccharomyces pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     CRISPR-Cas12a system in fission yeast for multiplex genomic editing 
            and CRISPR interference.
  JOURNAL   Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 
            10.1093/nar/gkaa329.
  PUBMED    32374858
REFERENCE   2  (bases 1 to 13329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-06-4- 4"; volume: "48"; issue: 
            "10"; pages: "5788-5798"
COMMENT     Sequence Label: pYZ714
FEATURES             Location/Qualifiers
     source          1..13329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..259
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1573..1770
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1831..1848)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1831..1847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1840..1862)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1988..2443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2075..2094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2285..2306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2879..3673)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      4293..5076
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        5262..6003
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             6030..6050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9972..10019
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      10971..11218
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10987..11005)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        11424..11528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11529..11597,11598..12389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11747..11766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12560..13148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13049..13068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13302..13319
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 aatacgactc actataggga aagcttgcat gcctgcaggt cgactctaga ggatcggccg
      301 gcccgcggcc gcacagtctc atactcttct agtcaagtct catcctcttc cactctctca
      361 attccttttt atcaatgttt cttcctcttg ttatgtacac aaacgatgat ctcttctctc
      421 acctcattgc tggactaata ttactcgtgg ccaagcgaaa cacaagtggt actagataac
      481 caaaaaatga taaagcattc aatcaatcaa accacttacg aaattcatac tcccgtaaac
      541 gaatgccggt cggacgcatg gaaaatcatc aatacgtgag agagctactt agttgtgcac
      601 tttgtatatg tccaaaaata cttttcattt cacatacctg cttgtgtaaa gacagtgatg
      661 aaatgcggtg caaagtgtct attcttacat tgatggtaaa gctaattcaa gcatgcttgg
      721 aagaagaatt gccttgcatt tcttgatcat ttgacccagg cgacaactat aaaataagaa
      781 tactaccgaa gatgcagatg gggtgtgtgg gcacaacacc atcgaaccga accgagacgg
      841 ccagactgtg ataacaaaag aaatgcatta gtttagttgg ctggttcaag ttggttgagt
      901 agaaaaatcc atcactcaaa ctgggaagta gggatggctg ggagaatgga gaagcgagac
      961 gagacgagac aagacaagag gagatgaaat gaaattaatg aaaagaaatg aatagaaatg
     1021 aaatagacat atgaatagta tagggcaggt tagatgtttg tatttataaa tgtttaatgc
     1081 ttaggtttgt atttatgttt acgttggtaa tgtttctttt gtttgtgttt gtgcatcggg
     1141 tgaacgagtt ggttaccgcc gccgccgctt ccttttgcct tgacacacac cacgcaacgt
     1201 gttacggttc ttgaacggaa ttgcgtactt cgatgcagag cgctatcggg gggattgggg
     1261 atggtaaatg gaagaaatca aaaacttctt ttgacgtcaa cccctaaaac aggggacggt
     1321 gattggtggt aaccacggac cggataggta tcgatatgca catcaggtga gaagtttttc
     1381 cgaggccaac tccggctcca tggtatataa tagagggttg cgcgctactg ttgcagtatc
     1441 atacttccaa aggtcgtata gaacacacac acaaattagc aaaatgaatt tctactgttg
     1501 tagatcgaga cctccctgca agaccatcta caaagttggt ctccaatttc tactgttgta
     1561 gattgagtca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     1621 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     1681 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     1741 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     1801 gtgtcgaagc ggccgcgcga gctcgaattt actggccgtc gttttacaac gtcgtgactg
     1861 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1921 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1981 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2341 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2401 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttagcttgt gatattgacg
     2461 aaactttttg acatctaatt tattctgttc caacaccaat gtttataacc aagttttatc
     2521 ttgtttgtct acatggtatt ttacattcat ctacatacat ctttcattgg ctttgtacat
     2581 agttatcatt acaagtctaa aaaaattcac tcttttctta ttcaatgtca atccaagaga
     2641 aaagattgtg gtaatgttgt aggagcatgt ttaataaatt actatagcaa attacttttt
     2701 attcccaagg tgtttatcta taatagttaa tattttagtc gctacataaa attttaccaa
     2761 agagtacttg tatactaatt ctaaatgcct tctgacataa aacgcctagg aaaacaaacg
     2821 caaacaaggc atcgactttt tcaataacca accaaaaaaa ttttacatta gtcttttttt
     2881 aatgctgaga aagtctttgc tgatatgcct tccaaccagc ttctctatat ctcttggctt
     2941 cgacaacagg attacgacca gctccataga ctccacgacc aacaatgatg atatcgctac
     3001 cgcagtttac aatcacttct tcaggagtac gatattgctg tcccagcccg tctcctttaa
     3061 catccaagcc gataccaggg gacatagtta tgtagtcgct ttgaaggtta ggaaatcgac
     3121 gaccagctat aaagccaaag caaaaatcgg tatgcttctc aaaccattct aaggttttct
     3181 ctgtgtagga accagtagcc aaagagcctt tggaagacat ttcagccaaa agcaagagtc
     3241 cacgtcccaa aggtaaacca acttctttga ggccttgtat aataccctcg cctggcactg
     3301 tatggcaatt tgtgatatga gcccaagaag caattttgta cacaccagat gcatattgta
     3361 gcttgacggt atttccaatg tctgcgaatt tgcgatcctc aaagataaga aaacgatgct
     3421 ttttacctaa ggccaccagt ttttctacca tatcctggtc gaaatcctcg acaacgtcaa
     3481 tatgtgtctt gataacacag acatagggtc caattttatc taccaattct aagatttcgg
     3541 atttcttcgt caaatcgacc gcgactgaca agttgctttg cttttcttcc atcaaagcca
     3601 acaattcctt ggcaatggga tttttcatcc cctcagctct agctgaatag ctttgaaata
     3661 ctctagcatc cataactttg cttttaaacc tttaatttcg atccaagcaa aaaagaggtt
     3721 cttggtagga caatacggta agaaaacacg acatgtgcag agatgccgac gaagcatagt
     3781 taaactggga tggtaaaatc aattaagaat ttataaagac aaaattgtat aagtctctaa
     3841 aacatcttaa ttatacctca cagaactatc taaaatatat tcacaaagtg caaacattat
     3901 catgaaaaag aaccatttta atttaaagca agggcattaa ggcttattta cagaatttct
     3961 tacttttgta aagattataa ggctgattat ctttttcacc atgccaaaaa ttacacaaga
     4021 tagaatggat gtttgaaatt aaacgtgagt atacaaacaa atacactagg taaatcgaaa
     4081 catttttttc tccattaagt aacaaattcc tatttagaga aagaatgctg agtagattaa
     4141 ataatctata caaacttttt taacacaaat gcatacatat agccagtggg atttgtagct
     4201 agtcgatcga ctctagagga aattcgagtc taactcctta accactcgga catagtgact
     4261 tatctgacac tcattgtaaa ttaatatata tataggcatt ttgtttagtt aaaggtactt
     4321 aagtaattag tataaacgaa ccaattttat aatcaggaag ttaagtgaat ggtagcacat
     4381 gtcgtaaaaa ttgtgaattt ttattgaata atattttaaa tacaagcctt tctagactag
     4441 gtatactcat aaacatatat gagcaaaagg atagaggaga ttacattgca tcttctacaa
     4501 atttatttat tgccctttac tgaaaaatta aataatgagt actaaatgat aaaaagcgct
     4561 cagtacaaga aagattgcaa aaatattgca ttcttcatga attaaagttg catataaggc
     4621 atattgaaag taatagtact aaaacagcag ttagcgaaaa ttaatagaat tatattcgca
     4681 agacaattgt gacattaaat taaaaaattg tagaattttt actatcctct ttaacgccat
     4741 gagcctttat aaaaaggtta aattagtttt aacattcttt ttttgagtaa gagtttacaa
     4801 tttatcaaaa cctgtgttat tatattcatt agtttcaatt tattagcatc tagagaaaaa
     4861 tcaattggca gttacattgt tggaatttat gaagaaaaag aatctacaac ggagaataag
     4921 ttgctgatcg ctttccctaa aattgtatat tttgctgagc ttattttgac atttcgttga
     4981 agttttctct aattcgcatt cattttaagt aaaacaatga gaaataaaat tacaaaaaat
     5041 acaaattaaa atacaatttt tagctataat tatagacgat gcccttgtat cccattctgt
     5101 ctcgcttgcc cctacttttt atcttttata taccataatg aacgctgccg ctactaacca
     5161 taccccgatt ttacatttcg gactcccaag gacgtacaaa atagaaaact atagaaaaaa
     5221 ataatcagaa aatagcatgt catctctttg taaaacgcag tgccctacaa caactaagaa
     5281 aatggctatc atgcggaagc tggtgagaag aacagcatcg ggacaaggga aggaagaaca
     5341 aagacaaaga aaacaaaaga aagcaattga aaacaaaaca aaacaatttt cattccttct
     5401 cttatcattc cttttctttt cttttctctc attcaacgca ctccatcgta tccgtattcc
     5461 tcttattttt tctctttctc tatatccatt tctttctctc taggtgtgtc ctctctctct
     5521 cttcaatttc tctactccgc attccaacgc atccttcccc caacctccca tttcctcctt
     5581 acggcccgat agcgatcgtc tttccctcgc tatcactcgc taccggcccc tcctctgcac
     5641 cgtaacctcc tacgtattta ccatatcata aagttttttc cgacgcttat cgctgacccc
     5701 ctgtcgccct cctattggct tccggattat cttcttgtcc ataaggtgat ccatgcttcc
     5761 tgaagattcc cgaaatgtgt ccactttggc ggggaatcat tccatccact tctttctctc
     5821 tcgctttcct cattcggcgc tccccttccg cgtctcattg gtcttccgct ccgtttttgc
     5881 tttgccgatg ttacttgggg agaggtgcga taatcctttc gcaaaaactc ggtttgacgc
     5941 ctcccatggt ataaatagtg ggtggtggac aggtgccttc gcttttcttt aagcaagaga
     6001 attgctgcag gtcgactcta gagatggctc ctaagaagaa gcggaaggtt ggtattcacg
     6061 gggtgcctgc ggctagcatc taccaggagt tcgtcaacaa gtattcactg agtaagacac
     6121 tgcggttcga gctgatccca cagggcaaga cactggagaa catcaaggcc cgaggcctga
     6181 ttctggacga tgagaagcgg gcaaaagact ataagaaagc caagcagatc attgataaat
     6241 accaccagtt ctttatcgag gaaattctga gctccgtgtg catcagtgag gatctgctgc
     6301 agaattactc agacgtgtac ttcaagctga agaagagcga cgatgacaac ctgcagaagg
     6361 acttcaagtc cgccaaggac accatcaaga aacagattag cgagtacatc aaggactccg
     6421 aaaagtttaa aaatctgttc aaccagaatc tgatcgatgc taagaaaggc caggagtccg
     6481 acctgatcct gtggctgaaa cagtctaagg acaatgggat tgaactgttc aaggctaact
     6541 ccgatatcac tgatattgac gaggcactgg aaatcatcaa gagcttcaag ggatggacca
     6601 catactttaa aggcttccac gagaaccgca agaacgtgta ctccagcaac gacattccta
     6661 cctccatcat ctaccgaatc gtcgatgaca atctgccaaa gttcctggag aacaaggcca
     6721 aatatgaatc tctgaaggac aaagctcccg aggcaattaa ttacgaacag atcaagaaag
     6781 atctggctga ggaactgaca ttcgatatcg actataagac tagcgaggtg aaccagaggg
     6841 tcttttccct ggacgaggtg tttgaaatcg ccaatttcaa caattacctg aaccagtccg
     6901 gcattactaa attcaatacc atcattggcg ggaagtttgt gaacggggag aataccaagc
     6961 gcaagggaat taacgaatac atcaatctgt atagccagca gatcaacgac aaaactctga
     7021 agaaatacaa gatgtctgtg ctgttcaaac agatcctgag tgataccgag tccaagtctt
     7081 ttgtcattga taaactggaa gatgactcag acgtggtcac taccatgcag agcttttatg
     7141 agcagatcgc cgctttcaag acagtggagg aaaaatctat taaggaaact ctgagtctgc
     7201 tgttcgatga cctgaaagcc cagaagctgg acctgagtaa gatctacttc aaaaacgata
     7261 agagtctgac agacctgtca cagcaggtgt ttgatgacta ttccgtgatt gggaccgccg
     7321 tcctggagta cattacacag cagatcgctc caaagaacct ggataatccc tctaagaaag
     7381 agcaggaact gatcgctaag aaaaccgaga aggcaaaata tctgagtctg gaaacaatta
     7441 agctggcact ggaggagttc aacaagcaca gggatattga caaacagtgc cgctttgagg
     7501 aaatcctggc caacttcgca gccatcccca tgatttttga tgagatcgcc cagaacaaag
     7561 acaatctggc tcagatcagt attaagtacc agaaccaggg caagaaagac ctgctgcagg
     7621 cttcagcaga agatgacgtg aaagccatca aggatctgct ggaccagacc aacaatctgc
     7681 tgcacaagct gaaaatcttc catattagtc agtcagagga taaggctaat atcctggata
     7741 aagacgaaca cttctacctg gtgttcgagg aatgttactt cgagctggca aacattgtcc
     7801 ccctgtataa caagattagg aactacatca cacagaagcc ttactctgac gagaagttta
     7861 aactgaactt cgaaaatagt accctggcca acgggtggga taagaacaag gagcctgaca
     7921 acacagctat cctgttcatc aaggatgaca agtactatct gggagtgatg aataagaaaa
     7981 acaataagat cttcgatgac aaagccatta aggagaacaa aggggaagga tacaagaaaa
     8041 tcgtgtataa gctgctgccc ggcgcaaata agatgctgcc taaggtgttc ttcagcgcca
     8101 agagtatcaa attctacaac ccatccgagg acatcctgcg gattagaaat cactcaacac
     8161 atactaagaa cgggagcccc cagaagggat atgagaaatt tgagttcaac atcgaggatt
     8221 gcaggaagtt tattgacttc tacaagcaga gcatctccaa acaccctgaa tggaaggatt
     8281 ttggcttccg gttttccgac acacagagat ataactctat cgacgagttc taccgcgagg
     8341 tggaaaatca ggggtataag ctgacttttg agaacatttc tgaaagttac atcgacagcg
     8401 tggtcaatca gggaaagctg tacctgttcc agatctataa caaagatttt tcagcataca
     8461 gcaagggcag accaaacctg catacactgt actggaaggc cctgttcgat gagaggaatc
     8521 tgcaggacgt ggtctataaa ctgaacggag aggccgaact gttttaccgg aagcagtcta
     8581 ttcctaagaa aatcactcac ccagctaagg aggccatcgc taacaagaac aaggacaatc
     8641 ctaagaaaga gagcgtgttc gaatacgatc tgattaagga caagcggttc accgaagata
     8701 agttcttttt ccattgtcca atcaccatta acttcaagtc aagcggcgct aacaagttca
     8761 acgacgagat caatctgctg ctgaaggaaa aagcaaacga tgtgcacatc ctgagcattg
     8821 cccgaggaga gcggcatctg gcctactata ccctggtgga tggcaaaggg aatatcatta
     8881 agcaggatac attcaacatc attggcaatg accggatgaa aaccaactac cacgataaac
     8941 tggctgcaat cgagaaggat agagactcag ctaggaagga ctggaagaaa atcaacaaca
     9001 ttaaggagat gaaggaaggc tatctgagcc aggtggtcca tgagattgca aagctggtca
     9061 tcgaatacaa tgccattgtg gtgttcgagg atctgaactt cggctttaag agggggcgct
     9121 ttaaggtgga aaaacaggtc tatcagaagc tggagaaaat gctgatcgaa aagctgaatt
     9181 acctggtgtt taaagataac gagttcgaca agaccggagg cgtcctgaga gcctaccagc
     9241 tgacagctcc ctttgaaact ttcaagaaaa tgggaaaaca gacaggcatc atctactatg
     9301 tgccagccgg attcacttcc aagatctgcc ccgtgaccgg ctttgtcaac cagctgtacc
     9361 ctaaatatga gtcagtgagc aagtcccagg aatttttcag caagttcgat aagatctgtt
     9421 ataatctgga caaggggtac ttcgagtttt ccttcgatta caagaacttc ggcgacaagg
     9481 ccgctaaggg gaaatggacc attgcctcct tcggatctcg cctgatcaac tttcgaaatt
     9541 ccgataaaaa ccacaattgg gacactaggg aggtgtaccc aaccaaggag ctggaaaagc
     9601 tgctgaaaga ctactctatc gagtatggac atggcgaatg catcaaggca gccatctgtg
     9661 gcgagagtga taagaaattt ttcgccaagc tgacctcagt gctgaataca atcctgcaga
     9721 tgcggaactc aaagaccggg acagaactgg actatctgat tagccccgtg gctgatgtca
     9781 acggaaactt cttcgacagc agacaggcac ccaaaaatat gcctcaggat gcagacgcca
     9841 acggggccta ccacatcggg ctgaagggac tgatgctgct gggccggatc aagaacaatc
     9901 aggaggggaa gaagctgaac ctggtcatta agaacgagga atacttcgag tttgtccaga
     9961 atagaaataa caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
    10021 gatccggtag ttccaagctt ggcggcagcg gcggcagctc caaacttgac tcttataccc
    10081 atctaagttt ttacgaaaag agagagctat ttcgtaagaa actgcgggaa attgaaggac
    10141 ccgaagtaac gcttgtcaat gaagttgacg atgaaccctg tccatcttta gattttcaat
    10201 tcattagcca gtaccgctta acacaaggtg ttatcccgcc tgatccaaat tttcaatctg
    10261 gatgcaattg ctcgtccctt ggcggttgtg accttaacaa tccttctcgt tgtgagtgtt
    10321 tagatgattt ggatgagcca actcattttg cctatgatgc acaggggcgg gtccgtgcag
    10381 atacgggtgc cgttatatat gaatgcaact ctttttgttc ttgttcaatg gagtgtccta
    10441 atcgcgttgt tcaacgtggt agaactcttc cgcttgaaat atttaaaaca aaagaaaaag
    10501 gatggggtgt acgttcgctt cgatttgccc ctgctggcac gtttatcact tgctatttag
    10561 gagaagtcat aacttcagca gaggccgcca agcgtgacaa aaattatgac gacgacggta
    10621 ttacctattt atttgattta gacatgtttg atgatgctag cgagtataca gtggatgctc
    10681 agaactatgg agatgtctct cgttttttta accactcctg ttcacctaat atagctattt
    10741 attctgctgt tcggaaccat ggatttcgaa ccatttatga tctcgctttt tttgccatca
    10801 aagacattca gcctctagag gagcttacat ttgattacgc tggtgcaaaa gatttctcac
    10861 ctgttcaatc tcaaaaatct cagcaaaata gaatttcaaa acttcgccgg cagtgcaaat
    10921 gtggttccgc taattgtcgt ggctggcttt tcggttaatc tcttctcgag tcatgtaatt
    10981 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
    11041 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
    11101 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
    11161 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcga
    11221 tccccttttc gtttgcaaga agaaaggaaa caatggagaa gagatcatcc atttgtatgt
    11281 aaattttagt aaacttgaag aaatcactaa caacttctct tacttaggga ttctatgcaa
    11341 aaccttgtaa atcatctgat ggaggactcg atttaatgaa ttggaaggtt ggatttccag
    11401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    11461 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11761 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12241 gtggatctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    12301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    12361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12421 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    12601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    12661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    12721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    13021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    13081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    13141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    13201 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    13261 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    13321 aagcggaag
//
