LOCUS       sequence-307975-       12397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in 
            Schizosaccharomyces pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12397)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     CRISPR-Cas12a system in fission yeast for multiplex genomic editing 
            and CRISPR interference.
  JOURNAL   Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 
            10.1093/nar/gkaa329.
  PUBMED    32374858
REFERENCE   2  (bases 1 to 12397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-06-4- 4"; volume: "48"; issue: 
            "10"; pages: "5788-5798"
COMMENT     Sequence Label: pYZ694
FEATURES             Location/Qualifiers
     source          1..12397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        325..681
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     misc_RNA        709..760
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(829..845)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(830..846)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(870..889)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(871..889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(899..916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(899..915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(908..930)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1056..1511
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1143..1162)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1353..1374
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1947..2741)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      3361..4144
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        4330..5071
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             5098..5118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9040..9087
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      10039..10286
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10055..10073)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        10492..10596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10597..10665,10666..11457)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10815..10834)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11628..12216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12117..12136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12370..12387
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctcgg atccactagt aacggccgcc
      301 agtgtgctgg aattcgccct tccttttgct tatgttggtg gtagttggca tgcgtagact
      361 gatgactagt cagcaaggag cgtagaacag tcacactcgt tatatatgtg cttccaagaa
      421 aactcaagaa tttaccatta gcaaacactt ttttgaaatg ttagacattt aaatgacgaa
      481 ggcatataga agctttgaat aggtgttgta aagtgttgat ttatgtgacg ctgagggtgc
      541 gcatgaaagg aatgttgggt cacgattatt aaacagtttg ctagcttgga cacttgagta
      601 ttggaagttg ttgaattcta aaaaactttc agttgatttg aatagttgct gttgccaaaa
      661 aacataacct gtaccgaaga aaatttctac tgttgtagat gcggccgccc tgtcaccgga
      721 tgtgctttcc ggtctgatga gtccgtgagg acgaaacagg ggtaccgtgg ggatcctcta
      781 gagtcgacct gaagggcgaa ttctgcagga attcgatatc aagcttatcg ataccgtcga
      841 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      901 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      961 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1021 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     1081 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1141 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1201 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1261 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1321 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1381 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     1441 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     1501 gtttacaatt tagcttgtga tattgacgaa actttttgac atctaattta ttctgttcca
     1561 acaccaatgt ttataaccaa gttttatctt gtttgtctac atggtatttt acattcatct
     1621 acatacatct ttcattggct ttgtacatag ttatcattac aagtctaaaa aaattcactc
     1681 ttttcttatt caatgtcaat ccaagagaaa agattgtggt aatgttgtag gagcatgttt
     1741 aataaattac tatagcaaat tactttttat tcccaaggtg tttatctata atagttaata
     1801 ttttagtcgc tacataaaat tttaccaaag agtacttgta tactaattct aaatgccttc
     1861 tgacataaaa cgcctaggaa aacaaacgca aacaaggcat cgactttttc aataaccaac
     1921 caaaaaaatt ttacattagt ctttttttaa tgctgagaaa gtctttgctg atatgccttc
     1981 caaccagctt ctctatatct cttggcttcg acaacaggat tacgaccagc tccatagact
     2041 ccacgaccaa caatgatgat atcgctaccg cagtttacaa tcacttcttc aggagtacga
     2101 tattgctgtc ccagcccgtc tcctttaaca tccaagccga taccagggga catagttatg
     2161 tagtcgcttt gaaggttagg aaatcgacga ccagctataa agccaaagca aaaatcggta
     2221 tgcttctcaa accattctaa ggttttctct gtgtaggaac cagtagccaa agagcctttg
     2281 gaagacattt cagccaaaag caagagtcca cgtcccaaag gtaaaccaac ttctttgagg
     2341 ccttgtataa taccctcgcc tggcactgta tggcaatttg tgatatgagc ccaagaagca
     2401 attttgtaca caccagatgc atattgtagc ttgacggtat ttccaatgtc tgcgaatttg
     2461 cgatcctcaa agataagaaa acgatgcttt ttacctaagg ccaccagttt ttctaccata
     2521 tcctggtcga aatcctcgac aacgtcaata tgtgtcttga taacacagac atagggtcca
     2581 attttatcta ccaattctaa gatttcggat ttcttcgtca aatcgaccgc gactgacaag
     2641 ttgctttgct tttcttccat caaagccaac aattccttgg caatgggatt tttcatcccc
     2701 tcagctctag ctgaatagct ttgaaatact ctagcatcca taactttgct tttaaacctt
     2761 taatttcgat ccaagcaaaa aagaggttct tggtaggaca atacggtaag aaaacacgac
     2821 atgtgcagag atgccgacga agcatagtta aactgggatg gtaaaatcaa ttaagaattt
     2881 ataaagacaa aattgtataa gtctctaaaa catcttaatt atacctcaca gaactatcta
     2941 aaatatattc acaaagtgca aacattatca tgaaaaagaa ccattttaat ttaaagcaag
     3001 ggcattaagg cttatttaca gaatttctta cttttgtaaa gattataagg ctgattatct
     3061 ttttcaccat gccaaaaatt acacaagata gaatggatgt ttgaaattaa acgtgagtat
     3121 acaaacaaat acactaggta aatcgaaaca tttttttctc cattaagtaa caaattccta
     3181 tttagagaaa gaatgctgag tagattaaat aatctataca aactttttta acacaaatgc
     3241 atacatatag ccagtgggat ttgtagctag tcgatcgact ctagaggaaa ttcgagtcta
     3301 actccttaac cactcggaca tagtgactta tctgacactc attgtaaatt aatatatata
     3361 taggcatttt gtttagttaa aggtacttaa gtaattagta taaacgaacc aattttataa
     3421 tcaggaagtt aagtgaatgg tagcacatgt cgtaaaaatt gtgaattttt attgaataat
     3481 attttaaata caagcctttc tagactaggt atactcataa acatatatga gcaaaaggat
     3541 agaggagatt acattgcatc ttctacaaat ttatttattg ccctttactg aaaaattaaa
     3601 taatgagtac taaatgataa aaagcgctca gtacaagaaa gattgcaaaa atattgcatt
     3661 cttcatgaat taaagttgca tataaggcat attgaaagta atagtactaa aacagcagtt
     3721 agcgaaaatt aatagaatta tattcgcaag acaattgtga cattaaatta aaaaattgta
     3781 gaatttttac tatcctcttt aacgccatga gcctttataa aaaggttaaa ttagttttaa
     3841 cattcttttt ttgagtaaga gtttacaatt tatcaaaacc tgtgttatta tattcattag
     3901 tttcaattta ttagcatcta gagaaaaatc aattggcagt tacattgttg gaatttatga
     3961 agaaaaagaa tctacaacgg agaataagtt gctgatcgct ttccctaaaa ttgtatattt
     4021 tgctgagctt attttgacat ttcgttgaag ttttctctaa ttcgcattca ttttaagtaa
     4081 aacaatgaga aataaaatta caaaaaatac aaattaaaat acaattttta gctataatta
     4141 tagacgatgc ccttgtatcc cattctgtct cgcttgcccc tactttttat cttttatata
     4201 ccataatgaa cgctgccgct actaaccata ccccgatttt acatttcgga ctcccaagga
     4261 cgtacaaaat agaaaactat agaaaaaaat aatcagaaaa tagcatgtca tctctttgta
     4321 aaacgcagtg ccctacaaca actaagaaaa tggctatcat gcggaagctg gtgagaagaa
     4381 cagcatcggg acaagggaag gaagaacaaa gacaaagaaa acaaaagaaa gcaattgaaa
     4441 acaaaacaaa acaattttca ttccttctct tatcattcct tttcttttct tttctctcat
     4501 tcaacgcact ccatcgtatc cgtattcctc ttattttttc tctttctcta tatccatttc
     4561 tttctctcta ggtgtgtcct ctctctctct tcaatttctc tactccgcat tccaacgcat
     4621 ccttccccca acctcccatt tcctccttac ggcccgatag cgatcgtctt tccctcgcta
     4681 tcactcgcta ccggcccctc ctctgcaccg taacctccta cgtatttacc atatcataaa
     4741 gttttttccg acgcttatcg ctgaccccct gtcgccctcc tattggcttc cggattatct
     4801 tcttgtccat aaggtgatcc atgcttcctg aagattcccg aaatgtgtcc actttggcgg
     4861 ggaatcattc catccacttc tttctctctc gctttcctca ttcggcgctc cccttccgcg
     4921 tctcattggt cttccgctcc gtttttgctt tgccgatgtt acttggggag aggtgcgata
     4981 atcctttcgc aaaaactcgg tttgacgcct cccatggtat aaatagtggg tggtggacag
     5041 gtgccttcgc ttttctttaa gcaagagaat tgctgcaggt cgactctaga gatggctcct
     5101 aagaagaagc ggaaggttgg tattcacggg gtgcctgcgg ctagcatcta ccaggagttc
     5161 gtcaacaagt attcactgag taagacactg cggttcgagc tgatcccaca gggcaagaca
     5221 ctggagaaca tcaaggcccg aggcctgatt ctggacgatg agaagcgggc aaaagactat
     5281 aagaaagcca agcagatcat tgataaatac caccagttct ttatcgagga aattctgagc
     5341 tccgtgtgca tcagtgagga tctgctgcag aattactcag acgtgtactt caagctgaag
     5401 aagagcgacg atgacaacct gcagaaggac ttcaagtccg ccaaggacac catcaagaaa
     5461 cagattagcg agtacatcaa ggactccgaa aagtttaaaa atctgttcaa ccagaatctg
     5521 atcgatgcta agaaaggcca ggagtccgac ctgatcctgt ggctgaaaca gtctaaggac
     5581 aatgggattg aactgttcaa ggctaactcc gatatcactg atattgacga ggcactggaa
     5641 atcatcaaga gcttcaaggg atggaccaca tactttaaag gcttccacga gaaccgcaag
     5701 aacgtgtact ccagcaacga cattcctacc tccatcatct accgaatcgt cgatgacaat
     5761 ctgccaaagt tcctggagaa caaggccaaa tatgaatctc tgaaggacaa agctcccgag
     5821 gcaattaatt acgaacagat caagaaagat ctggctgagg aactgacatt cgatatcgac
     5881 tataagacta gcgaggtgaa ccagagggtc ttttccctgg acgaggtgtt tgaaatcgcc
     5941 aatttcaaca attacctgaa ccagtccggc attactaaat tcaataccat cattggcggg
     6001 aagtttgtga acggggagaa taccaagcgc aagggaatta acgaatacat caatctgtat
     6061 agccagcaga tcaacgacaa aactctgaag aaatacaaga tgtctgtgct gttcaaacag
     6121 atcctgagtg ataccgagtc caagtctttt gtcattgata aactggaaga tgactcagac
     6181 gtggtcacta ccatgcagag cttttatgag cagatcgccg ctttcaagac agtggaggaa
     6241 aaatctatta aggaaactct gagtctgctg ttcgatgacc tgaaagccca gaagctggac
     6301 ctgagtaaga tctacttcaa aaacgataag agtctgacag acctgtcaca gcaggtgttt
     6361 gatgactatt ccgtgattgg gaccgccgtc ctggagtaca ttacacagca gatcgctcca
     6421 aagaacctgg ataatccctc taagaaagag caggaactga tcgctaagaa aaccgagaag
     6481 gcaaaatatc tgagtctgga aacaattaag ctggcactgg aggagttcaa caagcacagg
     6541 gatattgaca aacagtgccg ctttgaggaa atcctggcca acttcgcagc catccccatg
     6601 atttttgatg agatcgccca gaacaaagac aatctggctc agatcagtat taagtaccag
     6661 aaccagggca agaaagacct gctgcaggct tcagcagaag atgacgtgaa agccatcaag
     6721 gatctgctgg accagaccaa caatctgctg cacaagctga aaatcttcca tattagtcag
     6781 tcagaggata aggctaatat cctggataaa gacgaacact tctacctggt gttcgaggaa
     6841 tgttacttcg agctggcaaa cattgtcccc ctgtataaca agattaggaa ctacatcaca
     6901 cagaagcctt actctgacga gaagtttaaa ctgaacttcg aaaatagtac cctggccaac
     6961 gggtgggata agaacaagga gcctgacaac acagctatcc tgttcatcaa ggatgacaag
     7021 tactatctgg gagtgatgaa taagaaaaac aataagatct tcgatgacaa agccattaag
     7081 gagaacaaag gggaaggata caagaaaatc gtgtataagc tgctgcccgg cgcaaataag
     7141 atgctgccta aggtgttctt cagcgccaag agtatcaaat tctacaaccc atccgaggac
     7201 atcctgcgga ttagaaatca ctcaacacat actaagaacg ggagccccca gaagggatat
     7261 gagaaatttg agttcaacat cgaggattgc aggaagttta ttgacttcta caagcagagc
     7321 atctccaaac accctgaatg gaaggatttt ggcttccggt tttccgacac acagagatat
     7381 aactctatcg acgagttcta ccgcgaggtg gaaaatcagg ggtataagct gacttttgag
     7441 aacatttctg aaagttacat cgacagcgtg gtcaatcagg gaaagctgta cctgttccag
     7501 atctataaca aagatttttc agcatacagc aagggcagac caaacctgca tacactgtac
     7561 tggaaggccc tgttcgatga gaggaatctg caggacgtgg tctataaact gaacggagag
     7621 gccgaactgt tttaccggaa gcagtctatt cctaagaaaa tcactcaccc agctaaggag
     7681 gccatcgcta acaagaacaa ggacaatcct aagaaagaga gcgtgttcga atacgatctg
     7741 attaaggaca agcggttcac cgaagataag ttctttttcc attgtccaat caccattaac
     7801 ttcaagtcaa gcggcgctaa caagttcaac gacgagatca atctgctgct gaaggaaaaa
     7861 gcaaacgatg tgcacatcct gagcattgcc cgaggagagc ggcatctggc ctactatacc
     7921 ctggtggatg gcaaagggaa tatcattaag caggatacat tcaacatcat tggcaatgac
     7981 cggatgaaaa ccaactacca cgataaactg gctgcaatcg agaaggatag agactcagct
     8041 aggaaggact ggaagaaaat caacaacatt aaggagatga aggaaggcta tctgagccag
     8101 gtggtccatg agattgcaaa gctggtcatc gaatacaatg ccattgtggt gttcgaggat
     8161 ctgaacttcg gctttaagag ggggcgcttt aaggtggaaa aacaggtcta tcagaagctg
     8221 gagaaaatgc tgatcgaaaa gctgaattac ctggtgttta aagataacga gttcgacaag
     8281 accggaggcg tcctgagagc ctaccagctg acagctccct ttgaaacttt caagaaaatg
     8341 ggaaaacaga caggcatcat ctactatgtg ccagccggat tcacttccaa gatctgcccc
     8401 gtgaccggct ttgtcaacca gctgtaccct aaatatgagt cagtgagcaa gtcccaggaa
     8461 tttttcagca agttcgataa gatctgttat aatctggaca aggggtactt cgagttttcc
     8521 ttcgattaca agaacttcgg cgacaaggcc gctaagggga aatggaccat tgcctccttc
     8581 ggatctcgcc tgatcaactt tcgaaattcc gataaaaacc acaattggga cactagggag
     8641 gtgtacccaa ccaaggagct ggaaaagctg ctgaaagact actctatcga gtatggacat
     8701 ggcgaatgca tcaaggcagc catctgtggc gagagtgata agaaattttt cgccaagctg
     8761 acctcagtgc tgaatacaat cctgcagatg cggaactcaa agaccgggac agaactggac
     8821 tatctgatta gccccgtggc tgatgtcaac ggaaacttct tcgacagcag acaggcaccc
     8881 aaaaatatgc ctcaggatgc agacgccaac ggggcctacc acatcgggct gaagggactg
     8941 atgctgctgg gccggatcaa gaacaatcag gaggggaaga agctgaacct ggtcattaag
     9001 aacgaggaat acttcgagtt tgtccagaat agaaataaca aaaggccggc ggccacgaaa
     9061 aaggccggcc aggcaaaaaa gaaaaaggga tccggtagtt ccaagcttgg cggcagcggc
     9121 ggcagctcca aacttgactc ttatacccat ctaagttttt acgaaaagag agagctattt
     9181 cgtaagaaac tgcgggaaat tgaaggaccc gaagtaacgc ttgtcaatga agttgacgat
     9241 gaaccctgtc catctttaga ttttcaattc attagccagt accgcttaac acaaggtgtt
     9301 atcccgcctg atccaaattt tcaatctgga tgcaattgct cgtcccttgg cggttgtgac
     9361 cttaacaatc cttctcgttg tgagtgttta gatgatttgg atgagccaac tcattttgcc
     9421 tatgatgcac aggggcgggt ccgtgcagat acgggtgccg ttatatatga atgcaactct
     9481 ttttgttctt gttcaatgga gtgtcctaat cgcgttgttc aacgtggtag aactcttccg
     9541 cttgaaatat ttaaaacaaa agaaaaagga tggggtgtac gttcgcttcg atttgcccct
     9601 gctggcacgt ttatcacttg ctatttagga gaagtcataa cttcagcaga ggccgccaag
     9661 cgtgacaaaa attatgacga cgacggtatt acctatttat ttgatttaga catgtttgat
     9721 gatgctagcg agtatacagt ggatgctcag aactatggag atgtctctcg tttttttaac
     9781 cactcctgtt cacctaatat agctatttat tctgctgttc ggaaccatgg atttcgaacc
     9841 atttatgatc tcgctttttt tgccatcaaa gacattcagc ctctagagga gcttacattt
     9901 gattacgctg gtgcaaaaga tttctcacct gttcaatctc aaaaatctca gcaaaataga
     9961 atttcaaaac ttcgccggca gtgcaaatgt ggttccgcta attgtcgtgg ctggcttttc
    10021 ggttaatctc ttctcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc
    10081 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
    10141 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
    10201 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
    10261 tgggacgctc gaaggcttta atttgcgatc cccttttcgt ttgcaagaag aaaggaaaca
    10321 atggagaaga gatcatccat ttgtatgtaa attttagtaa acttgaagaa atcactaaca
    10381 acttctctta cttagggatt ctatgcaaaa ccttgtaaat catctgatgg aggactcgat
    10441 ttaatgaatt ggaaggttgg atttccaggt ggcacttttc ggggaaatgt gcgcggaacc
    10501 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    10561 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    10621 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10681 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10741 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10801 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    10861 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10921 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10981 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    11041 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    11101 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    11161 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    11221 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    11281 attgctgata aatctggagc cggtgagcgt ggatctcgcg gtatcattgc agcactgggg
    11341 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    11401 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    11461 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    11521 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    11581 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    11641 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11701 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11761 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11821 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11881 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11941 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    12001 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    12061 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    12121 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    12181 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    12241 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    12301 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    12361 accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
