LOCUS       sequence-30798-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(1-393)-N268D
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg gaggagccgc agtcagatcc
      121 tagcgtcgag ccccctctga gtcaggaaac attttcagac ctatggaaac tacttcctga
      181 aaacaacgtt ctgtccccct tgccgtccca agcaatggat gatttgatgc tgtccccgga
      241 cgatattgaa caatggttca ccgaagaccc aggtccagat gaagctccca gaatgccaga
      301 ggctgctccc cccgtggccc ctgcaccagc agctcctaca ccggcggccc ctgcaccagc
      361 cccctcctgg cccctgtcat cttctgtccc ttcccagaaa acctaccagg gcagctacgg
      421 tttccgtctg ggcttcttgc attctgggac agccaagtct gtgacttgca cgtactcccc
      481 tgccctcaac aagatgtttt gccaactggc caagacctgc cctgtgcagc tgtgggttga
      541 ttccacaccc ccgcccggca cccgcgtccg cgccatggcc atctacaagc agtcacagca
      601 catgacggag gttgtgaggc gctgccccca ccatgagcgc tgctcagata gcgatggtct
      661 ggcccctcct cagcatctta tccgagtgga aggaaatttg cgtgtggagt atttggatga
      721 cagaaacact tttcgacata gtgtggtggt gccctatgag ccgcctgagg ttggctctga
      781 ctgtaccacc atccactaca actacatgtg taacagttcc tgcatgggcg gcatgaaccg
      841 gaggcccatc ctcaccatca tcacactgga agactccagt ggtaatctac tgggacggga
      901 cagctttgag gtgcgtgttt gtgcc
//
