LOCUS       sequence-307989-       12432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a 
            Schizosaccharomyces pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     CRISPR-Cas12a system in fission yeast for multiplex genomic editing 
            and CRISPR interference.
  JOURNAL   Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 
            10.1093/nar/gkaa329.
  PUBMED    32374858
REFERENCE   2  (bases 1 to 12432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-06-4- 4"; volume: "48"; issue: 
            "10"; pages: "5788-5798"
COMMENT     Sequence Label: pYZ675
FEATURES             Location/Qualifiers
     source          1..12432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     248..267
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        248..266
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1581..1778
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1839..1856)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1839..1855)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1848..1870)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1996..2451
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2083..2102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2293..2314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2887..3681)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      4301..5084
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        5270..6011
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             9932..9979
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9986..10066
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      10082..10329
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10098..10116)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        10535..10639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10640..10708,10709..11500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10858..10877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11671..12259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12160..12179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12413..12430
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      241 caagctctaa tacgactcac tatagggaaa gcttgcatgc ctgcaggtcg actctagagg
      301 atcggccggc ccgcggccgc acagtctcat actcttctag tcaagtctca tcctcttcca
      361 ctctctcaat tcctttttat caatgtttct tcctcttgtt atgtacacaa acgatgatct
      421 cttctctcac ctcattgctg gactaatatt actcgtggcc aagcgaaaca caagtggtac
      481 tagataacca aaaaatgata aagcattcaa tcaatcaaac cacttacgaa attcatactc
      541 ccgtaaacga atgccggtcg gacgcatgga aaatcatcaa tacgtgagag agctacttag
      601 ttgtgcactt tgtatatgtc caaaaatact tttcatttca catacctgct tgtgtaaaga
      661 cagtgatgaa atgcggtgca aagtgtctat tcttacattg atggtaaagc taattcaagc
      721 atgcttggaa gaagaattgc cttgcatttc ttgatcattt gacccaggcg acaactataa
      781 aataagaata ctaccgaaga tgcagatggg gtgtgtgggc acaacaccat cgaaccgaac
      841 cgagacggcc agactgtgat aacaaaagaa atgcattagt ttagttggct ggttcaagtt
      901 ggttgagtag aaaaatccat cactcaaact gggaagtagg gatggctggg agaatggaga
      961 agcgagacga gacgagacaa gacaagagga gatgaaatga aattaatgaa aagaaatgaa
     1021 tagaaatgaa atagacatat gaatagtata gggcaggtta gatgtttgta tttataaatg
     1081 tttaatgctt aggtttgtat ttatgtttac gttggtaatg tttcttttgt ttgtgtttgt
     1141 gcatcgggtg aacgagttgg ttaccgccgc cgccgcttcc ttttgccttg acacacacca
     1201 cgcaacgtgt tacggttctt gaacggaatt gcgtacttcg atgcagagcg ctatcggggg
     1261 gattggggat ggtaaatgga agaaatcaaa aacttctttt gacgtcaacc cctaaaacag
     1321 gggacggtga ttggtggtaa ccacggaccg gataggtatc gatatgcaca tcaggtgaga
     1381 agtttttccg aggccaactc cggctccatg gtatataata gagggttgcg cgctactgtt
     1441 gcagtatcat acttccaaag gtcgtataga acacacacac aaattagcaa aatgaatttc
     1501 tactgttgta gatcgagacc tccctgcaag accatctaca aagttggtct ccaatttcta
     1561 ctgttgtaga ttgagtcagt actgacaata aaaagattct tgttttcaag aacttgtcat
     1621 ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata
     1681 ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat
     1741 catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc
     1801 gccatccagt gtcgaagcgg ccgcgcgagc tcgaatttac tggccgtcgt tttacaacgt
     1861 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1921 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1981 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2041 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2101 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2161 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2221 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2281 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     2341 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2401 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tagcttgtga
     2461 tattgacgaa actttttgac atctaattta ttctgttcca acaccaatgt ttataaccaa
     2521 gttttatctt gtttgtctac atggtatttt acattcatct acatacatct ttcattggct
     2581 ttgtacatag ttatcattac aagtctaaaa aaattcactc ttttcttatt caatgtcaat
     2641 ccaagagaaa agattgtggt aatgttgtag gagcatgttt aataaattac tatagcaaat
     2701 tactttttat tcccaaggtg tttatctata atagttaata ttttagtcgc tacataaaat
     2761 tttaccaaag agtacttgta tactaattct aaatgccttc tgacataaaa cgcctaggaa
     2821 aacaaacgca aacaaggcat cgactttttc aataaccaac caaaaaaatt ttacattagt
     2881 ctttttttaa tgctgagaaa gtctttgctg atatgccttc caaccagctt ctctatatct
     2941 cttggcttcg acaacaggat tacgaccagc tccatagact ccacgaccaa caatgatgat
     3001 atcgctaccg cagtttacaa tcacttcttc aggagtacga tattgctgtc ccagcccgtc
     3061 tcctttaaca tccaagccga taccagggga catagttatg tagtcgcttt gaaggttagg
     3121 aaatcgacga ccagctataa agccaaagca aaaatcggta tgcttctcaa accattctaa
     3181 ggttttctct gtgtaggaac cagtagccaa agagcctttg gaagacattt cagccaaaag
     3241 caagagtcca cgtcccaaag gtaaaccaac ttctttgagg ccttgtataa taccctcgcc
     3301 tggcactgta tggcaatttg tgatatgagc ccaagaagca attttgtaca caccagatgc
     3361 atattgtagc ttgacggtat ttccaatgtc tgcgaatttg cgatcctcaa agataagaaa
     3421 acgatgcttt ttacctaagg ccaccagttt ttctaccata tcctggtcga aatcctcgac
     3481 aacgtcaata tgtgtcttga taacacagac atagggtcca attttatcta ccaattctaa
     3541 gatttcggat ttcttcgtca aatcgaccgc gactgacaag ttgctttgct tttcttccat
     3601 caaagccaac aattccttgg caatgggatt tttcatcccc tcagctctag ctgaatagct
     3661 ttgaaatact ctagcatcca taactttgct tttaaacctt taatttcgat ccaagcaaaa
     3721 aagaggttct tggtaggaca atacggtaag aaaacacgac atgtgcagag atgccgacga
     3781 agcatagtta aactgggatg gtaaaatcaa ttaagaattt ataaagacaa aattgtataa
     3841 gtctctaaaa catcttaatt atacctcaca gaactatcta aaatatattc acaaagtgca
     3901 aacattatca tgaaaaagaa ccattttaat ttaaagcaag ggcattaagg cttatttaca
     3961 gaatttctta cttttgtaaa gattataagg ctgattatct ttttcaccat gccaaaaatt
     4021 acacaagata gaatggatgt ttgaaattaa acgtgagtat acaaacaaat acactaggta
     4081 aatcgaaaca tttttttctc cattaagtaa caaattccta tttagagaaa gaatgctgag
     4141 tagattaaat aatctataca aactttttta acacaaatgc atacatatag ccagtgggat
     4201 ttgtagctag tcgatcgact ctagaggaaa ttcgagtcta actccttaac cactcggaca
     4261 tagtgactta tctgacactc attgtaaatt aatatatata taggcatttt gtttagttaa
     4321 aggtacttaa gtaattagta taaacgaacc aattttataa tcaggaagtt aagtgaatgg
     4381 tagcacatgt cgtaaaaatt gtgaattttt attgaataat attttaaata caagcctttc
     4441 tagactaggt atactcataa acatatatga gcaaaaggat agaggagatt acattgcatc
     4501 ttctacaaat ttatttattg ccctttactg aaaaattaaa taatgagtac taaatgataa
     4561 aaagcgctca gtacaagaaa gattgcaaaa atattgcatt cttcatgaat taaagttgca
     4621 tataaggcat attgaaagta atagtactaa aacagcagtt agcgaaaatt aatagaatta
     4681 tattcgcaag acaattgtga cattaaatta aaaaattgta gaatttttac tatcctcttt
     4741 aacgccatga gcctttataa aaaggttaaa ttagttttaa cattcttttt ttgagtaaga
     4801 gtttacaatt tatcaaaacc tgtgttatta tattcattag tttcaattta ttagcatcta
     4861 gagaaaaatc aattggcagt tacattgttg gaatttatga agaaaaagaa tctacaacgg
     4921 agaataagtt gctgatcgct ttccctaaaa ttgtatattt tgctgagctt attttgacat
     4981 ttcgttgaag ttttctctaa ttcgcattca ttttaagtaa aacaatgaga aataaaatta
     5041 caaaaaatac aaattaaaat acaattttta gctataatta tagacgatgc ccttgtatcc
     5101 cattctgtct cgcttgcccc tactttttat cttttatata ccataatgaa cgctgccgct
     5161 actaaccata ccccgatttt acatttcgga ctcccaagga cgtacaaaat agaaaactat
     5221 agaaaaaaat aatcagaaaa tagcatgtca tctctttgta aaacgcagtg ccctacaaca
     5281 actaagaaaa tggctatcat gcggaagctg gtgagaagaa cagcatcggg acaagggaag
     5341 gaagaacaaa gacaaagaaa acaaaagaaa gcaattgaaa acaaaacaaa acaattttca
     5401 ttccttctct tatcattcct tttcttttct tttctctcat tcaacgcact ccatcgtatc
     5461 cgtattcctc ttattttttc tctttctcta tatccatttc tttctctcta ggtgtgtcct
     5521 ctctctctct tcaatttctc tactccgcat tccaacgcat ccttccccca acctcccatt
     5581 tcctccttac ggcccgatag cgatcgtctt tccctcgcta tcactcgcta ccggcccctc
     5641 ctctgcaccg taacctccta cgtatttacc atatcataaa gttttttccg acgcttatcg
     5701 ctgaccccct gtcgccctcc tattggcttc cggattatct tcttgtccat aaggtgatcc
     5761 atgcttcctg aagattcccg aaatgtgtcc actttggcgg ggaatcattc catccacttc
     5821 tttctctctc gctttcctca ttcggcgctc cccttccgcg tctcattggt cttccgctcc
     5881 gtttttgctt tgccgatgtt acttggggag aggtgcgata atcctttcgc aaaaactcgg
     5941 tttgacgcct cccatggtat aaatagtggg tggtggacag gtgccttcgc ttttctttaa
     6001 gcaagagaat tgctgcaggt cgactctaga gatgagcatc taccaggagt tcgtcaacaa
     6061 gtattcactg agtaagacac tgcggttcga gctgatccca cagggcaaga cactggagaa
     6121 catcaaggcc cgaggcctga ttctggacga tgagaagcgg gcaaaagact ataagaaagc
     6181 caagcagatc attgataaat accaccagtt ctttatcgag gaaattctga gctccgtgtg
     6241 catcagtgag gatctgctgc agaattactc agacgtgtac ttcaagctga agaagagcga
     6301 cgatgacaac ctgcagaagg acttcaagtc cgccaaggac accatcaaga aacagattag
     6361 cgagtacatc aaggactccg aaaagtttaa aaatctgttc aaccagaatc tgatcgatgc
     6421 taagaaaggc caggagtccg acctgatcct gtggctgaaa cagtctaagg acaatgggat
     6481 tgaactgttc aaggctaact ccgatatcac tgatattgac gaggcactgg aaatcatcaa
     6541 gagcttcaag ggatggacca catactttaa aggcttccac gagaaccgca agaacgtgta
     6601 ctccagcaac gacattccta cctccatcat ctaccgaatc gtcgatgaca atctgccaaa
     6661 gttcctggag aacaaggcca aatatgaatc tctgaaggac aaagctcccg aggcaattaa
     6721 ttacgaacag atcaagaaag atctggctga ggaactgaca ttcgatatcg actataagac
     6781 tagcgaggtg aaccagaggg tcttttccct ggacgaggtg tttgaaatcg ccaatttcaa
     6841 caattacctg aaccagtccg gcattactaa attcaatacc atcattggcg ggaagtttgt
     6901 gaacggggag aataccaagc gcaagggaat taacgaatac atcaatctgt atagccagca
     6961 gatcaacgac aaaactctga agaaatacaa gatgtctgtg ctgttcaaac agatcctgag
     7021 tgataccgag tccaagtctt ttgtcattga taaactggaa gatgactcag acgtggtcac
     7081 taccatgcag agcttttatg agcagatcgc cgctttcaag acagtggagg aaaaatctat
     7141 taaggaaact ctgagtctgc tgttcgatga cctgaaagcc cagaagctgg acctgagtaa
     7201 gatctacttc aaaaacgata agagtctgac agacctgtca cagcaggtgt ttgatgacta
     7261 ttccgtgatt gggaccgccg tcctggagta cattacacag cagatcgctc caaagaacct
     7321 ggataatccc tctaagaaag agcaggaact gatcgctaag aaaaccgaga aggcaaaata
     7381 tctgagtctg gaaacaatta agctggcact ggaggagttc aacaagcaca gggatattga
     7441 caaacagtgc cgctttgagg aaatcctggc caacttcgca gccatcccca tgatttttga
     7501 tgagatcgcc cagaacaaag acaatctggc tcagatcagt attaagtacc agaaccaggg
     7561 caagaaagac ctgctgcagg cttcagcaga agatgacgtg aaagccatca aggatctgct
     7621 ggaccagacc aacaatctgc tgcacaagct gaaaatcttc catattagtc agtcagagga
     7681 taaggctaat atcctggata aagacgaaca cttctacctg gtgttcgagg aatgttactt
     7741 cgagctggca aacattgtcc ccctgtataa caagattagg aactacatca cacagaagcc
     7801 ttactctgac gagaagttta aactgaactt cgaaaatagt accctggcca acgggtggga
     7861 taagaacaag gagcctgaca acacagctat cctgttcatc aaggatgaca agtactatct
     7921 gggagtgatg aataagaaaa acaataagat cttcgatgac aaagccatta aggagaacaa
     7981 aggggaagga tacaagaaaa tcgtgtataa gctgctgccc ggcgcaaata agatgctgcc
     8041 taaggtgttc ttcagcgcca agagtatcaa attctacaac ccatccgagg acatcctgcg
     8101 gattagaaat cactcaacac atactaagaa cgggagcccc cagaagggat atgagaaatt
     8161 tgagttcaac atcgaggatt gcaggaagtt tattgacttc tacaagcaga gcatctccaa
     8221 acaccctgaa tggaaggatt ttggcttccg gttttccgac acacagagat ataactctat
     8281 cgacgagttc taccgcgagg tggaaaatca ggggtataag ctgacttttg agaacatttc
     8341 tgaaagttac atcgacagcg tggtcaatca gggaaagctg tacctgttcc agatctataa
     8401 caaagatttt tcagcataca gcaagggcag accaaacctg catacactgt actggaaggc
     8461 cctgttcgat gagaggaatc tgcaggacgt ggtctataaa ctgaacggag aggccgaact
     8521 gttttaccgg aagcagtcta ttcctaagaa aatcactcac ccagctaagg aggccatcgc
     8581 taacaagaac aaggacaatc ctaagaaaga gagcgtgttc gaatacgatc tgattaagga
     8641 caagcggttc accgaagata agttcttttt ccattgtcca atcaccatta acttcaagtc
     8701 aagcggcgct aacaagttca acgacgagat caatctgctg ctgaaggaaa aagcaaacga
     8761 tgtgcacatc ctgagcattg cccgaggaga gcggcatctg gcctactata ccctggtgga
     8821 tggcaaaggg aatatcatta agcaggatac attcaacatc attggcaatg accggatgaa
     8881 aaccaactac cacgataaac tggctgcaat cgagaaggat agagactcag ctaggaagga
     8941 ctggaagaaa atcaacaaca ttaaggagat gaaggaaggc tatctgagcc aggtggtcca
     9001 tgagattgca aagctggtca tcgaatacaa tgccattgtg gtgttcgagg atctgaactt
     9061 cggctttaag agggggcgct ttaaggtgga aaaacaggtc tatcagaagc tggagaaaat
     9121 gctgatcgaa aagctgaatt acctggtgtt taaagataac gagttcgaca agaccggagg
     9181 cgtcctgaga gcctaccagc tgacagctcc ctttgaaact ttcaagaaaa tgggaaaaca
     9241 gacaggcatc atctactatg tgccagccgg attcacttcc aagatctgcc ccgtgaccgg
     9301 ctttgtcaac cagctgtacc ctaaatatga gtcagtgagc aagtcccagg aatttttcag
     9361 caagttcgat aagatctgtt ataatctgga caaggggtac ttcgagtttt ccttcgatta
     9421 caagaacttc ggcgacaagg ccgctaaggg gaaatggacc attgcctcct tcggatctcg
     9481 cctgatcaac tttcgaaatt ccgataaaaa ccacaattgg gacactaggg aggtgtaccc
     9541 aaccaaggag ctggaaaagc tgctgaaaga ctactctatc gagtatggac atggcgaatg
     9601 catcaaggca gccatctgtg gcgagagtga taagaaattt ttcgccaagc tgacctcagt
     9661 gctgaataca atcctgcaga tgcggaactc aaagaccggg acagaactgg actatctgat
     9721 tagccccgtg gctgatgtca acggaaactt cttcgacagc agacaggcac ccaaaaatat
     9781 gcctcaggat gcagacgcca acggggccta ccacatcggg ctgaagggac tgatgctgct
     9841 gggccggatc aagaacaatc aggaggggaa gaagctgaac ctggtcatta agaacgagga
     9901 atacttcgag tttgtccaga atagaaataa caaaaggccg gcggccacga aaaaggccgg
     9961 ccaggcaaaa aagaaaaagg gatcctaccc atacgatgtt ccagattacg cttatcccta
    10021 cgacgtgcct gattatgcat acccatatga tgtccccgac tatgcctaat ctcttctcga
    10081 gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
    10141 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
    10201 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
    10261 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
    10321 ttaatttgcg atcccctttt cgtttgcaag aagaaaggaa acaatggaga agagatcatc
    10381 catttgtatg taaattttag taaacttgaa gaaatcacta acaacttctc ttacttaggg
    10441 attctatgca aaaccttgta aatcatctga tggaggactc gatttaatga attggaaggt
    10501 tggatttcca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10561 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10621 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10681 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10741 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10801 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10861 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    10921 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10981 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    11041 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    11101 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    11161 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    11221 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    11281 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    11341 agccggtgag cgtggatctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11401 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    11461 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11521 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11581 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    11821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    12121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    12181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    12241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    12301 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    12361 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    12421 agtgagcgag ga
//
