LOCUS       sequence-30802-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(1-393)-M340Q-L344R
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatggagg agccgcagtc agatcctagc
      121 gtcgagcccc ctctgagtca ggaaacattt tcagacctat ggaaactact tcctgaaaac
      181 aacgttctgt cccccttgcc gtcccaagca atggatgatt tgatgctgtc cccggacgat
      241 attgaacaat ggttcaccga agacccaggt ccagatgaag ctcccagaat gccagaggct
      301 gctccccccg tggcccctgc accagcagct cctacaccgg cggcccctgc accagccccc
      361 tcctggcccc tgtcatcttc tgtcccttcc cagaaaacct accagggcag ctacggtttc
      421 cgtctgggct tcttgcattc tgggacagcc aagtctgtga cttgcacgta ctcccctgcc
      481 ctcaacaaga tgttttgcca actggccaag acctgccctg tgcagctgtg ggttgattcc
      541 acacccccgc ccggcacccg cgtccgcgcc atggccatct acaagcagtc acagcacatg
      601 acggaggttg tgaggcgctg cccccaccat gagcgctgct cagatagcga tggtctggcc
      661 cctcctcagc atcttatccg agtggaagga aatttgcgtg tggagtattt ggatgacaga
      721 aacacttttc gacatagtgt ggtggtgccc tatgagccgc ctgaggttgg ctctgactgt
      781 accaccatcc actacaacta catgtgtaac agttcctgca tgggcggcat gaaccggagg
      841 cccatcctca ccatcatcac actggaagac tccagtggta atctactggg acggaacagc
      901 tttgaggtgc gtgtttgtgc ctgtcctggg agaaac
//
