LOCUS       sequence-308020-        5380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luminescent reporter for abscisic acid signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Lehmann S, Dominguez-Ferreras A, Huang WJ, Denby K, Ntoukakis V, 
            Schafer P
  TITLE     Novel markers for high-throughput protoplast-based analyses of 
            phytohormone signaling.
  JOURNAL   PLoS One. 2020 Jun 4;15(6):e0234154. doi: 
            10.1371/journal.pone.0234154. eCollection 2020.
  PUBMED    32497144
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-06-4"; volume: "15(6):e0234154. doi"; pages: " 
            10.1371/journal.pone.0234154. eCollection 2020"
COMMENT     Sequence Label: pRAB18::LUC
FEATURES             Location/Qualifiers
     source          1..5380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..30)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..30)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(27..49)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(62..68,69..86,87..92))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(245..262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(416..1004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(496..515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1175..1966,1967..2035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1798..1817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2036..2140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2208..2226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2386..2405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2599..2621
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2613..2630
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2614..2630
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3316..4968
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3382..3400)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5123..5375
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
       61 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      121 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      241 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      301 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      361 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      421 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      481 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      541 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      601 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      661 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      721 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      781 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      841 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      901 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      961 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1021 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1081 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1141 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1201 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1261 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1321 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1381 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1441 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1501 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1561 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1621 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1681 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1741 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1801 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1861 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1921 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1981 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     2041 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2101 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2161 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2221 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2281 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2341 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2401 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2461 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2521 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2581 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg
     2641 catgcctgca ggctctagag gatccggtgc agagaggaga aactccccga caatgaacac
     2701 caacgatcta aacgcggcgt ttggtaaaag ttgagtaaat tttgttaggg cttagtttta
     2761 gtccatgggc taattagtaa gtgatttacg gcccacacat gagcccaaat gtttcagacc
     2821 cagccaagtt tcttcaaatt cacccaatca acgacgatgt acgtgtgtat gaaaatcatt
     2881 aacacgacgc atcgctttcg aggaggagca ttacgtgtcc tgttagctac gataatgtta
     2941 gtaccgccac aaagaaaagg atagatattt tgctttccag caccctgtca tgggattgat
     3001 atgaacacgt acttggtatc gacatgaaag ctcaaaaata aattcaatcc gattccttta
     3061 gtgatatcag aagttcattt taaatacgaa cacgtatggc gaaacaccac gccgacattt
     3121 tctgctgctg ccacgcgtca ctttccaaat attgattcat taaactaata gttgatccat
     3181 atccgaaacc ggactataaa actatcttca atgcgttaac gaatcttcat cgatcaaact
     3241 catcaaagtc taatatcaca aagaaagagt ttttttaact agcttagctc aaagtgtttg
     3301 cttaagacaa gaaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     3361 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     3421 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
     3481 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     3541 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     3601 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     3661 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
     3721 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
     3781 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     3841 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
     3901 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
     3961 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     4021 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     4081 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
     4141 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
     4201 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
     4261 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     4321 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     4381 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     4441 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     4501 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     4561 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     4621 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     4681 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     4741 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     4801 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     4861 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     4921 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
     4981 ttcagcgatg acgaaattct tagctattgt aatattatat gcaaattgat gaatggtaat
     5041 tttgtaattg tgggtcactg tactatttta acgaataata aaatcaggta taggtaacta
     5101 aaaaggaatt cgagctcgaa ttgatcgttc aaacatttgg caataaagtt tcttaagatt
     5161 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     5221 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     5281 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     5341 attatcgcgc gcggtgtcat ctatgttact agatctctag
//
