LOCUS       sequence-30804-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(1-320)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taattccctc tgaatatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg aggagccgca
      121 gtcagatcct agcgtcgagc cccctctgag tcaggaaaca ttttcagacc tatggaaact
      181 acttcctgaa aacaacgttc tgtccccctt gccgtcccaa gcaatggatg atttgatgct
      241 gtccccggac gatattgaac aatggttcac cgaagaccca ggtccagatg aagctcccag
      301 aatgccagag gctgctcccc ccgtggcccc tgcaccagca gctcctacac cggcggcccc
      361 tgcaccagcc ccctcctggc ccctgtcatc ttctgtccct tcccagaaaa cctaccaggg
      421 cagctacggt ttccgtctgg gcttcttgca ttctgggaca gccaagtctg tgacttgcac
      481 gtactcccct gccctcaaca agatgttttg ccaactggcc aagacctgcc ctgtgcagct
      541 gtgggttgat tccacacccc cgcccggcac ccgcgtccgc gccatggcca tctacaagca
      601 gtcacagcac atgacggagg ttgtgaggcg ctgcccccac catgagcgct gctcagatag
      661 cgatggtctg gcccctcctc agcatcttat ccgagtggaa ggaaatttgc gtgtggagta
      721 tttggatgac agaaacactt ttcgacatag tgtggtggtg ccctatgagc cgcctgaggt
      781 tggctctgac tgtaccacca tccactacaa ctacatgtgt aacagttcct gcatgggcgg
      841 catgaaccgg aggcccatcc tcaccatcat cacact
//
