LOCUS       sequence-308046-        8851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SEAP and firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni
            G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, 
            Kempa S, Huttelmaier S, Selbach M, Landthaler M
  TITLE     HDLBP binds ER-targeted mRNAs by multivalent interactions to promote
            protein synthesis of transmembrane and secreted proteins.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7.
  PUBMED    35585045
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2727. doi"; pages: " 
            10.1038/s41467-022-30322-7"
COMMENT     Sequence Label: pFRTpsiCHECK_SEAP
FEATURES             Location/Qualifiers
     source          1..8851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          109..241
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     224..243
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        286..304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             310..1812
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    1870..1918
                     /note="synthetic polyadenylation signal"
     promoter        1927..2678
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2708..2717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2714..4366
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3673..3681
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(4412..4429)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4418..4642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4775..4794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4926..4973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4981..6003
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4981..6003
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6133..6254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6170..6189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6224..6243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6303..6319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6303..6319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6316..6338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6327..6343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6351..6357,6358..6375,6376..6381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6396..6417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6534..6551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6705..7293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6785..6804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7464..8255,8256..8324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8087..8106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8325..8429)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(8533..8851,1..39)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8533..8724
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8556..8576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(8741..8851,1..25)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca
       61 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      121 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      181 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      241 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
      301 taggctagca tgctgctgct gctgctgctg ctgggcctga ggctacagct ctccctgggc
      361 atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
      421 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
      481 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
      541 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
      601 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
      661 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
      721 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
      781 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
      841 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
      901 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
      961 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac
     1021 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
     1081 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
     1141 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
     1201 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
     1261 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
     1321 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
     1381 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
     1441 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
     1501 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
     1561 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
     1621 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
     1681 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
     1741 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
     1801 ggcaccaccg acgccgcgca cccgggttac tctagagtcg gggcggccgg ctaggcggcc
     1861 gctggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgag
     1921 gatctaaatg agtcttcgga cctcgcgggg gccgcttaag cggtggttag ggtttgtctg
     1981 acgcgggggg agggggaagg aacgaaacac tctcattcgg aggcggctcg gggtttggtc
     2041 ttggtggcca cgggcacgca gaagagcgcc gcgatcctct taagcacccc cccgccctcc
     2101 gtggaggcgg gggtttggtc ggcgggtggt aactggcggg ccgctgactc gggcgggtcg
     2161 cgcgccccag agtgtgacct tttcggtctg ctcgcagacc cccgggcggc gccgccgcgg
     2221 cggcgacggg ctcgctgggt cctaggctcc atggggaccg tatacgtgga caggctctgg
     2281 agcatccgca cgactgcggt gatattaccg gagaccttct gcgggacgag ccgggtcacg
     2341 cggctgacgc ggagcgtccg ttgggcgaca aacaccagga cggggcacag gtacactatc
     2401 ttgtcacccg gaggcgcgag ggactgcagg agcttcaggg agtggcgcag ctgcttcatc
     2461 cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc
     2521 tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg
     2581 cagatgcagt cggggcggcg cggtcccagg tccacttcgc atattaaggt gacgcgtgtg
     2641 gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaaa gcttggcatt ccggtactgt
     2701 tggtaaagcc accatggccg atgctaagaa cattaagaag ggccctgctc ccttctaccc
     2761 tctggaggat ggcaccgctg gcgagcagct gcacaaggcc atgaagaggt atgccctggt
     2821 gcctggcacc attgccttca ccgatgccca cattgaggtg gacatcacct atgccgagta
     2881 cttcgagatg tctgtgcgcc tggccgaggc catgaagagg tacggcctga acaccaacca
     2941 ccgcatcgtg gtgtgctctg agaactctct gcagttcttc atgccagtgc tgggcgccct
     3001 gttcatcgga gtggccgtgg cccctgctaa cgacatttac aacgagcgcg agctgctgaa
     3061 cagcatgggc atttctcagc ctaccgtggt gttcgtgtct aagaagggcc tgcagaagat
     3121 cctgaacgtg cagaagaagc tgcctatcat ccagaagatc atcatcatgg actctaagac
     3181 cgactaccag ggcttccaga gcatgtacac attcgtgaca tctcatctgc ctcctggctt
     3241 caacgagtac gacttcgtgc cagagtcttt cgacagggac aaaaccattg ccctgatcat
     3301 gaacagctct gggtctaccg gcctgcctaa gggcgtggcc ctgcctcatc gcaccgcctg
     3361 tgtgcgcttc tctcacgccc gcgaccctat tttcggcaac cagatcatcc ccgacaccgc
     3421 tattctgagc gtggtgccat tccaccacgg cttcggcatg ttcaccaccc tgggctacct
     3481 gatttgcggc tttcgggtgg tgctgatgta ccgcttcgag gaggagctgt tcctgcgcag
     3541 cctgcaagac tacaaaattc agtctgccct gctggtgcca accctgttca gcttcttcgc
     3601 taagagcacc ctgatcgaca agtacgacct gtctaacctg cacgagattg cctctggcgg
     3661 cgccccactg tctaaggagg tgggcgaagc cgtggccaag cgctttcatc tgccaggcat
     3721 ccgccagggc tacggcctga ccgagacaac cagcgccatt ctgattaccc cagagggcga
     3781 cgacaagcct ggcgccgtgg gcaaggtggt gccattcttc gaggccaagg tggtggacct
     3841 ggacaccggc aagaccctgg gagtgaacca gcgcggcgag ctgtgtgtgc gcggccctat
     3901 gattatgtcc ggctacgtga ataaccctga ggccacaaac gccctgatcg acaaggacgg
     3961 ctggctgcac tctggcgaca ttgcctactg ggacgaggac gagcacttct tcatcgtgga
     4021 ccgcctgaag tctctgatca agtacaaggg ctaccaggtg gccccagccg agctggagtc
     4081 tatcctgctg cagcacccta acattttcga cgccggagtg gccggcctgc ccgacgacga
     4141 tgccggcgag ctgcctgccg ccgtcgtcgt gctggaacac ggcaagacca tgaccgagaa
     4201 ggagatcgtg gactatgtgg ccagccaggt gacaaccgcc aagaagctgc gcggcggagt
     4261 ggtgttcgtg gacgaggtgc ccaagggcct gaccggcaag ctggacgccc gcaagatccg
     4321 cgagatcctg atcaaggcta agaaaggcgg caagatcgcc gtgtaataat tctagtcgag
     4381 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4441 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4501 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4561 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4621 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4681 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4741 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4801 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4861 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4921 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
     4981 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     5041 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     5101 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     5161 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     5221 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     5281 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     5341 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5401 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5461 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5521 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5581 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5641 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5701 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     5761 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5821 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     5881 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     5941 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     6001 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6061 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6121 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6241 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8461 gacggatcgg gagatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     8521 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     8581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8821 ggccgcctcg gcctctgagc tattccagaa g
//
