LOCUS       sequence-308055-       11333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9K(-10TC)_arr2
FEATURES             Location/Qualifiers
     source          1..11333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(3371..3385,3386..3391,3392..3408,3409..3415)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3391..3407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3423..3439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3458..3469
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(3476..3502,3503..3505,3506..5992,5993..5995,
                     5996..7582)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7752..8561
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7819..7838)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8684..9272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9173..9192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtttagaat tatattcatt tttttcaaat tctttttgag tgttgcatag aatttccttt
       61 aatttttctt gtttttcttt catacaagat ttagtaaaat gtatattttt attttttagt
      121 aatttaattt ctattatttt tatgatttta agtattttga ttatattatc tttatcaata
      181 tttaaattga ataaaattgt aagaaaaatt gaaagaattt ttttacataa aaagttacat
      241 ttattgaagt aatttcttac ttgatacttt tctatttcat taatctttct ttcttctttt
      301 atattatttt tattatttaa acactccttt aaatctacac tcccattttt agtaaatcta
      361 tctttaaggc cccttgaaac tctattttga aatcttaaat cttttttttc ttgaaagtat
      421 ttatagattt tttggtaaca ttcttttttt gaaaaattaa gttgatagta tatttcggtc
      481 cccaaattta cccctaagtg tttgtgataa ttagttgtga ctttaaattc tttttctaat
      541 ttgtaaaggt aattttgcag tgtttttagt ttaacgggtg tttgactatt tcttttaagg
      601 ttttcattaa agtaatagag tatgtttttt tgtgtgtatt tcttatatgt tttatttatg
      661 tacgctagtg ttgagataag gactactaat ttatactgta atttattgta gcaagttgtg
      721 gttttactgg ttttgtttat attagataag ttttccattt atagctcctt gaataccgga
      781 ttttttatct ataaagtata tatatcacaa ttttgattta aaagtaaatt ctttttttaa
      841 aaatacaaaa attttttata aattatatag accatgatat tgtattaata ctaattgatt
      901 ttaatttaag gttgaactaa aataaacata gttttgtaga aaataatttt tcattatttt
      961 gctacttgat attggatcgt aaaaatatta gggctttaca aagttctttt aaaagagaat
     1021 ttagtaaagc cctaagtctt ttaataaaaa attttttgta aaaaagttgg caaaaatagt
     1081 ttttgctata tatttatata taagaaaaaa ttcaatactc aaggagataa aaaatggaaa
     1141 acttaaacaa taatccacaa aaaattaatc aaataaataa gcaaactaat tttattggtt
     1201 ttgaaaattt tatagaactt gataatcaag ttaatcaaac taaagaaaaa agtaaattaa
     1261 gtaagatagg taagaaatta cccggaataa gcagtcaagt ctagtgcggc cgcatggctt
     1321 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1381 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1441 gccctaaaac aaagttaaac atcatgagga ggtaacgtca tggtaaaaga ttggattcct
     1501 atttctcatg ataattataa acaagtacaa ggaccatttt atcatggaac taaagctaat
     1561 ttagcaattg gtgatttact aactacagga tttatttctc attttgaaga tggtcgaatt
     1621 cttaaacata tttatttttc agctttaatg gaaccagcag tttggggagc tgaacttgct
     1681 atgtcactat ctggtcttga aggtcgtggt tatatatata tagttgaacc aacaggacca
     1741 tttgaagatg atccaaatct tacaaataaa aaatttcctg gtaatccaac acaatcttat
     1801 agaacttgtg aacctttaag aattgttggt gttgttgaag attgggaagg acatcctgtt
     1861 gaattaataa gaggaatgtt agattcatta gaagatttaa aacgtcgtgg tttacatgtt
     1921 attgaagatt aacctggcgc gccaaataat gcccgtgtcc ctgttgaata gggacacggt
     1981 tttttaacaa tttttttatt cctaggcctt agtggccggt acccggggat ccttattact
     2041 ggccgctttc tagcctggat acttgtctgg ctagttgcat tagagagtca gctagagccc
     2101 tagggctagc tgtttgtgta ttcggagcca gtgtagtttt tctttttact aacgatacgg
     2161 gtaataattg attgcctttt acagcctgac cttgagacaa ttgcaaaagt ctgtccacag
     2221 aggtttgacc taataatcta aaatcttgtt tgatagtagt aagtggaggt atgtagcaac
     2281 tagaatcttc tgtatcatca taacccacaa ctgaaatatc agccccaact ctaagtccac
     2341 tttcagtaat agctcgcata gctcctaaag ccatttgatc attagcaact aacatagcag
     2401 tagggacaat gccttcattt aacatttgca ttgtttgctg aaatcctgac atagcagacc
     2461 aatctccttc tctttctgct attggttgta tttgatttct tgttaaatac ttgtgccatc
     2521 ctgctaatct caatctggca cttactgaac ttaagggacc tgcaagtagt gcaatttgtt
     2581 gatggcctaa tgcaacaagg tgttcaactc ctaaccttgt tccatcttcg tgagaaaaaa
     2641 ttatagagtt tattggggtt tggtccgaca catccaaaaa caatgctgga acattagtac
     2701 atgctgcttc aactgcaatc gcatcttggt catcaagagg ataattaatt ataagtccac
     2761 taacacgttg agctaataaa ttatgtactg ctgccttaca agcctctaca cctgatctct
     2821 caaccattga tacaacaacg cttgcaccaa gttgatcagc tctcgattta attgcggcta
     2881 caatttgact aggcgcatgt aatgcaagtg atgatgtggc aactccaatt aaaagtgatt
     2941 gtttccccgc aagttgctga gcaacgcgat ttggtatata attcaactct gccatagctg
     3001 cttcaacctt ctctcttgtt tttgcagata catgacttgc ttggttcaca actctcgaaa
     3061 ccgtttggta gctaaccccg gcatattcag caacatcata caaagtaaca ggtttcatgg
     3121 atcattcctc catgataaaa tttaaatttc tgactttggc aaatcctttt gcctgttaaa
     3181 aagaatagca caaaaattaa aaaaaaagaa acgcaaatag caatagcatt aaaaataatt
     3241 attattattt atttctttga ttaaaatcaa aagcctttaa tgtattttct tgatctttca
     3301 tgcgcttaac ggtaaaatcc aaggtattaa gttcaccttc cacttttctc tctctatctt
     3361 ctctagaaaa tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taacagattc
     3421 aattgtgagc ggataacaat ttcacacaga attcattaaa gaggagaaat tacatatgga
     3481 taagaaatac tcaataggct tagctatcgg cacaaatagc gtcggatggg cggtgatcac
     3541 tgatgaatat aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag
     3601 tatcaaaaaa aatcttatag gggctctttt atttgacagt ggagagacag cggaagcgac
     3661 tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg aagaatcgta tttgttatct
     3721 acaggagatt ttttcaaatg agatggcgaa agtagatgat agtttctttc atcgacttga
     3781 agagtctttt ttggtggaag aagacaagaa gcatgaacgt catcctattt ttggaaatat
     3841 agtagatgaa gttgcttatc atgagaaata tccaactatc tatcatctgc gaaaaaaatt
     3901 ggtagattct actgataaag cggatttgcg cttaatctat ttggccttag cgcatatgat
     3961 taagtttcgt ggtcattttt tgattgaggg agatttaaat cctgataata gtgatgtgga
     4021 caaactattt atccagttgg tacaaaccta caatcaatta tttgaagaaa accctattaa
     4081 cgcaagtgga gtagatgcta aagcgattct ttctgcacga ttgagtaaat caagacgatt
     4141 agaaaatctc attgctcagc tccccggtga gaagaaaaat ggcttatttg ggaatctcat
     4201 tgctttgtca ttgggtttga cccctaattt taaatcaaat tttgatttgg cagaagatgc
     4261 taaattacag ctttcaaaag atacttacga tgatgattta gataatttat tggcgcaaat
     4321 tggagatcaa tatgctgatt tgtttttggc agctaagaat ttatcagatg ctattttact
     4381 ttcagatatc ctaagagtaa atactgaaat aactaaggct cccctatcag cttcaatgat
     4441 taaacgctac gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca
     4501 acttccagaa aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta
     4561 tattgatggg ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa
     4621 aatggatggt actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca
     4681 acggaccttt gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat
     4741 tttgagaaga caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa
     4801 aatcttgact tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt
     4861 tgcatggatg actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt
     4921 cgataaaggt gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct
     4981 tccaaatgaa aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa
     5041 cgaattgaca aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg
     5101 tgaacagaag aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa
     5161 gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg
     5221 agttgaagat agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa
     5281 agataaagat tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac
     5341 attgacctta tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct
     5401 ctttgatgat aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt
     5461 gtctcgaaaa ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt
     5521 tttgaaatca gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt
     5581 gacatttaaa gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga
     5641 acatattgca aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa
     5701 agttgttgat gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga
     5761 aatggcacgt gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa
     5821 acgaatcgaa gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga
     5881 aaatactcaa ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat
     5941 gtatgtggac caagaattag atattaatcg tttaagtgat tatgatgtcg atgccattgt
     6001 tccacaaagt ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa
     6061 aaatcgtggt aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta
     6121 ttggagacaa cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa
     6181 agctgaacgt ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt
     6241 tgaaactcgc caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa
     6301 atacgatgaa aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt
     6361 agtttctgac ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca
     6421 tcatgcccat gatgcgtatc taaatgccgt cgttggaact gctttgatta agaaatatcc
     6481 aaaacttgaa tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat
     6541 tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat
     6601 catgaacttc ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct
     6661 aatcgaaact aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac
     6721 agtgcgcaaa gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtccagac
     6781 aggcggattc tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg
     6841 taaaaaagac tgggatccaa aaaaatatgg tggttttgat agtccaacgg tagcttattc
     6901 agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga
     6961 gttactaggg atcacaattc tggaaagaag ttcctttgaa aaaaatccga ttgacttttt
     7021 agaagctaaa ggatataagg aagttaaaaa agacttaatc attaaactac ctaaatatag
     7081 tctttttgag ttagaaaacg gtcgtaaacg gatgctggct agtgccggag aattacaaaa
     7141 aggaaatgag ctggctctgc caagcaaata tgtgaatttt ttatatttag ctagtcatta
     7201 tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa caattgtttg tggagcagca
     7261 taagcattat ttagatgaga ttattgagca aatcagtgaa ttttctaagc gtgttatttt
     7321 agcagatgcc aatttagata aagttcttag tgcatataac aaacatagag acaaaccaat
     7381 acgtgaacaa gcagaaaata ttattcattt atttacgttg acgaatcttg gagctcccgc
     7441 tgcttttaaa tattttgata caacaattga tcgtaaacga tatacgtcta caaaagaagt
     7501 tttagatgcc actcttatcc atcaatccat cactggtctt tatgaaacac gcattgattt
     7561 gagtcagcta ggaggtgact aataaaagct tgatcagatc ccagcttttt tttgaagtgc
     7621 ctggcagtaa gttgaaaaaa tttgaaataa acttggacta tgttaataaa aaaatacaaa
     7681 ttaacgaaca aaaagatttg aattattata taatttagtt aaaagcaatt ttaaatgagg
     7741 gaggtttcca tatgggccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     7801 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     7861 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     7921 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     7981 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     8041 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     8101 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     8161 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     8221 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     8281 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     8341 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     8401 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     8461 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     8521 ataaattgca gtttcatttg atgctcgatg agtttttcta atgacgtcgc cggagcggtc
     8581 gagttctgga ccgaccggct cgggttctca tgaccaaaat cccttaacgt gagttttcgt
     8641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcactagat ccccggcaag
     9361 ttcatcctta gggttttcat atctcttaaa aagtcaattt ctgcttgatt aatttcttgt
     9421 ggattattgt ttttgcggtt tttcattttt tatctccttg agtattgaat tttttcttat
     9481 atataaatat atagcaaaaa ctatttttgc caactttttt acaaaaaatt ttttattaaa
     9541 acacttaggg ctttgctaaa ttctctttta aaagaactta gtaaagccct atggatttaa
     9601 gaactgatta tacttacgta gtaaaaatac tacagatatt aatagtatag accatatcaa
     9661 aatcataatc aatttatatt tatctaaaat tgtagtccat acaaaataca aaaaaattag
     9721 gtttttctaa attctatttt aaaagaactt agtaacacct aatctctaaa ttacttacaa
     9781 aattctaaaa ttagattttt agttcttcat atttctttaa aagtttttta agaaaatctt
     9841 tttgattttc aaaaatttca tgcatcataa aactagtaaa tttggatttt tttttgtaaa
     9901 aatcataact ttcttgggtt ttaagttgaa atcttaatgg ttttattgga ttttgcctag
     9961 atttttttaa cgccggactt tctttatctt ttataaaatt ataagaattt ttaataccat
    10021 tttctattaa ataatcttct tttataagcc cctcttgcag tgcagttgct aatcttaaat
    10081 acttatacgc ttgagttttt gctacatcaa agtcttttat aaaatcaaga aaagttttat
    10141 aaccatcaaa tttataatat tgattatctt ttatttcttt taaaatttta atattttcca
    10201 ccctgtggaa tatttctttt ttgaaattgt catttaactt ttcctttagt gtcttgtaat
    10261 aagaaatttt actttcatta atcattaaat tttgttcaac ataagtatct atcttttttg
    10321 ttaaaacttt aacaacacgt tttttttcca taaaaatttt cctttatttt tgcaacattt
    10381 tccttaattg atataattgt tactatttta gattttttaa tatccactta agaattaatc
    10441 cctcactaga atatttttta ttatagaatt catatacatg tttttctaaa tctataaatt
    10501 tatcaacaat tgctttatta tagggtgtat ctatcttttc ttttcttaat aatcgtctca
    10561 tattctttaa ataacaacaa acactgccgg ttttaaacct aaattctatg taatatattt
    10621 ttgaaaaagt atatacattt tcaaccccgt ttatttcata ctttacaaaa acattattta
    10681 ttggttttct ataaccatag taaatgccta aaaatttgtc cccgtctttc ataggatata
    10741 gataaagttt ttgaatctct gatttatcta ataatttttc aaaaaaaatt atacaccgct
    10801 tttgtttgtt actaactact ctatgcaatg cctttaatat ttttgtgtga tatatatttt
    10861 tatcaccttt tttttctttc agcaggaaga tactttcttt tggaaattta attttttgtt
    10921 catttattct tgcagcctta agtccgattt tcatatttaa tccttatata gcctttctat
    10981 ttaattcttt taaactcaat aaactttttt ggtttttcat tagttctaat aattcgcaat
    11041 aatatgtgca aaacacttta ttgtattcta ttttcttttg gttattcaaa tattctttta
    11101 taattggctt tagaagttca atatttactt ctttttttag ttgttcaata aggatattga
    11161 aaatgtttgt ttttaaatct tgataatttt gttgagaatt ttcttttttt ctttcaattg
    11221 acttctctaa tttacgcttt atattgttta aatcgctata tttatgactt tcaataataa
    11281 aatgaggctt aaatttgtaa ttttcgtata ttttttggaa acttgtttct aat
//
