LOCUS       sequence-308056-       11333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9K(RBSmut)_arr2
FEATURES             Location/Qualifiers
     source          1..11333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(86..100,101..106,107..123,124..130)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    138..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(191..217,218..220,221..2707,2708..2710,2711..4297)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4467..5276
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4534..4553)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5399..5987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5888..5907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcttgatc tttcatgcgc ttaacggtaa aatccaaggt attaagttca ccttccactt
       61 ttctctctct atcttctcta gaaaatcata aaaaatttat ttgctttgtg agcggataac
      121 aattataata gattcaattg tgagcggata acaatttcac acagaattca ttaaagaaga
      181 gaaattacat atggataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg
      241 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa
      301 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga
      361 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa
      421 tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt
      481 ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc
      541 tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca
      601 tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc
      661 cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga
      721 taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga
      781 agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag
      841 taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt
      901 atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga
      961 tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa
     1021 tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc
     1081 agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct
     1141 atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc
     1201 tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa
     1261 cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa
     1321 accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga
     1381 tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg
     1441 tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg
     1501 tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg
     1561 tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa
     1621 ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa
     1681 ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta
     1741 ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc
     1801 agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg
     1861 aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag
     1921 tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt
     1981 gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga
     2041 ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa
     2101 aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac
     2161 tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa
     2221 aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat
     2281 ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg
     2341 cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat
     2401 tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga
     2461 aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc
     2521 gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa
     2581 agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca
     2641 aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga
     2701 tgtcgatgcc attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt
     2761 aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa
     2821 aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt
     2881 tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat
     2941 caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag
     3001 tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac
     3061 cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga
     3121 gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt
     3181 gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga
     3241 tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt
     3301 cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat
     3361 tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg
     3421 gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa
     3481 aacagaagtc cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga
     3541 caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc
     3601 aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt
     3661 aaaatccgtt aaagagttac tagggatcac aattctggaa agaagttcct ttgaaaaaaa
     3721 tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa
     3781 actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc
     3841 cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata
     3901 tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt
     3961 gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc
     4021 taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca
     4081 tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa
     4141 tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac
     4201 gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga
     4261 aacacgcatt gatttgagtc agctaggagg tgactaataa aagcttgatc agatcccagc
     4321 ttttttttga agtgcctggc agtaagttga aaaaatttga aataaacttg gactatgtta
     4381 ataaaaaaat acaaattaac gaacaaaaag atttgaatta ttatataatt tagttaaaag
     4441 caattttaaa tgagggaggt ttccatatgg gccatattca acgggaaacg tcgaggccgc
     4501 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     4561 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     4621 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     4681 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     4741 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     4801 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     4861 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     4921 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     4981 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     5041 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     5101 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     5161 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     5221 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatgac
     5281 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcatgacc aaaatccctt
     5341 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5401 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5461 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5521 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5581 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5641 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5701 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5761 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5821 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5881 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5941 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6001 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcac
     6061 tagatccccg gcaagttcat ccttagggtt ttcatatctc ttaaaaagtc aatttctgct
     6121 tgattaattt cttgtggatt attgtttttg cggtttttca ttttttatct ccttgagtat
     6181 tgaatttttt cttatatata aatatatagc aaaaactatt tttgccaact tttttacaaa
     6241 aaatttttta ttaaaacact tagggctttg ctaaattctc ttttaaaaga acttagtaaa
     6301 gccctatgga tttaagaact gattatactt acgtagtaaa aatactacag atattaatag
     6361 tatagaccat atcaaaatca taatcaattt atatttatct aaaattgtag tccatacaaa
     6421 atacaaaaaa attaggtttt tctaaattct attttaaaag aacttagtaa cacctaatct
     6481 ctaaattact tacaaaattc taaaattaga tttttagttc ttcatatttc tttaaaagtt
     6541 ttttaagaaa atctttttga ttttcaaaaa tttcatgcat cataaaacta gtaaatttgg
     6601 attttttttt gtaaaaatca taactttctt gggttttaag ttgaaatctt aatggtttta
     6661 ttggattttg cctagatttt tttaacgccg gactttcttt atcttttata aaattataag
     6721 aatttttaat accattttct attaaataat cttcttttat aagcccctct tgcagtgcag
     6781 ttgctaatct taaatactta tacgcttgag tttttgctac atcaaagtct tttataaaat
     6841 caagaaaagt tttataacca tcaaatttat aatattgatt atcttttatt tcttttaaaa
     6901 ttttaatatt ttccaccctg tggaatattt cttttttgaa attgtcattt aacttttcct
     6961 ttagtgtctt gtaataagaa attttacttt cattaatcat taaattttgt tcaacataag
     7021 tatctatctt ttttgttaaa actttaacaa cacgtttttt ttccataaaa attttccttt
     7081 atttttgcaa cattttcctt aattgatata attgttacta ttttagattt tttaatatcc
     7141 acttaagaat taatccctca ctagaatatt ttttattata gaattcatat acatgttttt
     7201 ctaaatctat aaatttatca acaattgctt tattataggg tgtatctatc ttttcttttc
     7261 ttaataatcg tctcatattc tttaaataac aacaaacact gccggtttta aacctaaatt
     7321 ctatgtaata tatttttgaa aaagtatata cattttcaac cccgtttatt tcatacttta
     7381 caaaaacatt atttattggt tttctataac catagtaaat gcctaaaaat ttgtccccgt
     7441 ctttcatagg atatagataa agtttttgaa tctctgattt atctaataat ttttcaaaaa
     7501 aaattataca ccgcttttgt ttgttactaa ctactctatg caatgccttt aatatttttg
     7561 tgtgatatat atttttatca cctttttttt ctttcagcag gaagatactt tcttttggaa
     7621 atttaatttt ttgttcattt attcttgcag ccttaagtcc gattttcata tttaatcctt
     7681 atatagcctt tctatttaat tcttttaaac tcaataaact tttttggttt ttcattagtt
     7741 ctaataattc gcaataatat gtgcaaaaca ctttattgta ttctattttc ttttggttat
     7801 tcaaatattc ttttataatt ggctttagaa gttcaatatt tacttctttt tttagttgtt
     7861 caataaggat attgaaaatg tttgttttta aatcttgata attttgttga gaattttctt
     7921 tttttctttc aattgacttc tctaatttac gctttatatt gtttaaatcg ctatatttat
     7981 gactttcaat aataaaatga ggcttaaatt tgtaattttc gtatattttt tggaaacttg
     8041 tttctaattg tttagaatta tattcatttt tttcaaattc tttttgagtg ttgcatagaa
     8101 tttcctttaa tttttcttgt ttttctttca tacaagattt agtaaaatgt atatttttat
     8161 tttttagtaa tttaatttct attattttta tgattttaag tattttgatt atattatctt
     8221 tatcaatatt taaattgaat aaaattgtaa gaaaaattga aagaattttt ttacataaaa
     8281 agttacattt attgaagtaa tttcttactt gatacttttc tatttcatta atctttcttt
     8341 cttcttttat attattttta ttatttaaac actcctttaa atctacactc ccatttttag
     8401 taaatctatc tttaaggccc cttgaaactc tattttgaaa tcttaaatct tttttttctt
     8461 gaaagtattt atagattttt tggtaacatt ctttttttga aaaattaagt tgatagtata
     8521 tttcggtccc caaatttacc cctaagtgtt tgtgataatt agttgtgact ttaaattctt
     8581 tttctaattt gtaaaggtaa ttttgcagtg tttttagttt aacgggtgtt tgactatttc
     8641 ttttaaggtt ttcattaaag taatagagta tgtttttttg tgtgtatttc ttatatgttt
     8701 tatttatgta cgctagtgtt gagataagga ctactaattt atactgtaat ttattgtagc
     8761 aagttgtggt tttactggtt ttgtttatat tagataagtt ttccatttat agctccttga
     8821 ataccggatt ttttatctat aaagtatata tatcacaatt ttgatttaaa agtaaattct
     8881 ttttttaaaa atacaaaaat tttttataaa ttatatagac catgatattg tattaatact
     8941 aattgatttt aatttaaggt tgaactaaaa taaacatagt tttgtagaaa ataatttttc
     9001 attattttgc tacttgatat tggatcgtaa aaatattagg gctttacaaa gttcttttaa
     9061 aagagaattt agtaaagccc taagtctttt aataaaaaat tttttgtaaa aaagttggca
     9121 aaaatagttt ttgctatata tttatatata agaaaaaatt caatactcaa ggagataaaa
     9181 aatggaaaac ttaaacaata atccacaaaa aattaatcaa ataaataagc aaactaattt
     9241 tattggtttt gaaaatttta tagaacttga taatcaagtt aatcaaacta aagaaaaaag
     9301 taaattaagt aagataggta agaaattacc cggaataagc agtcaagtct agtgcggccg
     9361 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     9421 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     9481 gggcagtcgc cctaaaacaa agttaaacat catgaggagg taacgtcatg gtaaaagatt
     9541 ggattcctat ttctcatgat aattataaac aagtacaagg accattttat catggaacta
     9601 aagctaattt agcaattggt gatttactaa ctacaggatt tatttctcat tttgaagatg
     9661 gtcgaattct taaacatatt tatttttcag ctttaatgga accagcagtt tggggagctg
     9721 aacttgctat gtcactatct ggtcttgaag gtcgtggtta tatatatata gttgaaccaa
     9781 caggaccatt tgaagatgat ccaaatctta caaataaaaa atttcctggt aatccaacac
     9841 aatcttatag aacttgtgaa cctttaagaa ttgttggtgt tgttgaagat tgggaaggac
     9901 atcctgttga attaataaga ggaatgttag attcattaga agatttaaaa cgtcgtggtt
     9961 tacatgttat tgaagattaa cctggcgcgc caaataatgc ccgtgtccct gttgaatagg
    10021 gacacggttt tttaacaatt tttttattcc taggccttag tggccggtac ccggggatcc
    10081 ttattactgg ccgctttcta gcctggatac ttgtctggct agttgcatta gagagtcagc
    10141 tagagcccta gggctagctg tttgtgtatt cggagccagt gtagtttttc tttttactaa
    10201 cgatacgggt aataattgat tgccttttac agcctgacct tgagacaatt gcaaaagtct
    10261 gtccacagag gtttgaccta ataatctaaa atcttgtttg atagtagtaa gtggaggtat
    10321 gtagcaacta gaatcttctg tatcatcata acccacaact gaaatatcag ccccaactct
    10381 aagtccactt tcagtaatag ctcgcatagc tcctaaagcc atttgatcat tagcaactaa
    10441 catagcagta gggacaatgc cttcatttaa catttgcatt gtttgctgaa atcctgacat
    10501 agcagaccaa tctccttctc tttctgctat tggttgtatt tgatttcttg ttaaatactt
    10561 gtgccatcct gctaatctca atctggcact tactgaactt aagggacctg caagtagtgc
    10621 aatttgttga tggcctaatg caacaaggtg ttcaactcct aaccttgttc catcttcgtg
    10681 agaaaaaatt atagagttta ttggggtttg gtccgacaca tccaaaaaca atgctggaac
    10741 attagtacat gctgcttcaa ctgcaatcgc atcttggtca tcaagaggat aattaattat
    10801 aagtccacta acacgttgag ctaataaatt atgtactgct gccttacaag cctctacacc
    10861 tgatctctca accattgata caacaacgct tgcaccaagt tgatcagctc tcgatttaat
    10921 tgcggctaca atttgactag gcgcatgtaa tgcaagtgat gatgtggcaa ctccaattaa
    10981 aagtgattgt ttccccgcaa gttgctgagc aacgcgattt ggtatataat tcaactctgc
    11041 catagctgct tcaaccttct ctcttgtttt tgcagataca tgacttgctt ggttcacaac
    11101 tctcgaaacc gtttggtagc taaccccggc atattcagca acatcataca aagtaacagg
    11161 tttcatggat cattcctcca tgataaaatt taaatttctg actttggcaa atccttttgc
    11221 ctgttaaaaa gaatagcaca aaaattaaaa aaaaagaaac gcaaatagca atagcattaa
    11281 aaataattat tattatttat ttctttgatt aaaatcaaaa gcctttaatg tat
//
