LOCUS       sequence-308057-        6743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luminescent reporter for jasmonic acid signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Lehmann S, Dominguez-Ferreras A, Huang WJ, Denby K, Ntoukakis V, 
            Schafer P
  TITLE     Novel markers for high-throughput protoplast-based analyses of 
            phytohormone signaling.
  JOURNAL   PLoS One. 2020 Jun 4;15(6):e0234154. doi: 
            10.1371/journal.pone.0234154. eCollection 2020.
  PUBMED    32497144
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-06-4"; volume: "15(6):e0234154. doi"; pages: " 
            10.1371/journal.pone.0234154. eCollection 2020"
COMMENT     Sequence Label: pJAZ10::LUC
FEATURES             Location/Qualifiers
     source          1..6743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..30)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..30)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(27..49)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(62..68,69..86,87..92))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(245..262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(416..1004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(496..515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1175..1966,1967..2035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1798..1817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2036..2140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2208..2226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2386..2405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2599..2621
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2613..2630
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2614..2630
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4679..6331
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4745..4763)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6486..6738
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
       61 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      121 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      241 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      301 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      361 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      421 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      481 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      541 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      601 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      661 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      721 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      781 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      841 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      901 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      961 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1021 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1081 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1141 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1201 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1261 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1321 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1381 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1441 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1501 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1561 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1621 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1681 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1741 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1801 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1861 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1921 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1981 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     2041 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2101 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2161 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2221 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2281 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2341 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2401 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2461 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2521 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2581 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg
     2641 catgcctgca ggctctagag gatcccaact ataggttcaa ttatattctt atgtaagggt
     2701 actaactagt gtgggctgta aacgacttaa agtttcggcc atttagtttt atgcaagaaa
     2761 aaaaatatag ataatacagt aatgatgaat ccactataca tgttaaatcc aatataataa
     2821 atccactata cattagtgtt ctcatatttc gtttcaagat tcgattcaaa aaatttgaaa
     2881 atgttaaata tgtgtttctg agattaaagg ccaaaagaag ggtaaggtcg tatatgaaag
     2941 gtggacagag gggcaaatta gtcagcttga aaaatacatc gacattaaaa atcggcagca
     3001 ctgacaaaac gtgtgataac acaaataaaa ataatcggaa gaaaaagaat aaataaggcg
     3061 tattgactct ggaacgtgag aagatgaaag agagcgacga aaacctctaa agacttatcg
     3121 agaagtgcac cttagcctag tgaataatga tatgtattga cattaactac tactactaca
     3181 gctcccaaca cgtgaaagac atgcatctaa ctctttcact ttcggttgcc aagaaaaaaa
     3241 aaaaaaaaaa actttcactt tcggcatcaa atatatacta ctaaaaaagt tttttatacg
     3301 taattctttt ttaatatttg attagttcag aatattaatt aaaaaaatta cttttcgtaa
     3361 tcactacatt ttgtagttat atgattttta atttaaaata gtaaaaagga gaaatgtaac
     3421 taattaagct aacatgcgcg cgatagaata aggtgaagtt taaatttgag cacaagatag
     3481 aaaattgagg tcaatttaat tcggaagtgg ttcaaggtat attttcacaa gaaagaaaaa
     3541 cgtgagttca ttgaatccct ctataacttt acttgagaac aatgtaataa tatgtaaatc
     3601 tgttaggaaa cgagcagcca catatgcttt aaacaagaga acaagaaaaa gagcggccac
     3661 atgtgaaaaa aaaacaccag ccaaaatgtg aaactaagaa aagaaaagaa aaaccatcga
     3721 catcgtccaa aggatcgacc cgtcttttct ggtttggcag aaaacaaact tccaagttcc
     3781 gacttgacct agtctttatg catctgcagt ggtgttttct ttaaatcttt ttttgaatgg
     3841 atttgaaaat cttatttttt cttcttcttt caaaacacta actgtgaatc accaccaaca
     3901 ttttattgtt ctaaaatccc tatttgtatg ttttattggt tactttccca aatatgatga
     3961 aagtttattg gtgtacttta gtttagaaat gtaagaaatt tcagttgtat tattaggtca
     4021 accaaatatt attcttgaat agtaatgtca aattttgttt atattgaaaa atcaataaga
     4081 ctgatatcta ctaaatgtga tttgctatac tagtagagct agtagtagca agattaaaat
     4141 tcatattaag aaaaaatgga aaagaaaaac atgtttcgag aaaaatatac agatcagaga
     4201 ctagagtaga aaacaaatat aatgtcagaa gaacccaatg tgacgaacag taacagaaac
     4261 atgttaccaa atttttgaag atatattttt ataaaacatt acaatgttca ttggacttcc
     4321 gttatttccg gatataaaat atcttctttc tgcgtagcaa gcaattacat tacattaggt
     4381 atattaagaa tttaaacatg tgttttgaat cattaaatta attagataag taagagaaga
     4441 ggttgggagc ctaataaaaa caaaacaaaa aggaaaaagt ggataggttg ccaattgtgc
     4501 cgaaaagaaa gagaaagcat gaacctcgtg cgttaaataa gtatcagaga catactacat
     4561 tttaaaaact ctcacatgag aaatcagaat ccgttattat tcctccattt attcatctca
     4621 aaacccatat ctctctgtct tgatctctct ctcactttct aataagatca aagaagccat
     4681 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag aggatggaac
     4741 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     4801 ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt
     4861 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     4921 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     4981 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc
     5041 gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     5101 aaaattacca ataatccaga aaattattat catggattct aaaacggatt accagggatt
     5161 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     5221 tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc
     5281 tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca
     5341 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     5401 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     5461 agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa
     5521 aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat
     5581 tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa
     5641 agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg
     5701 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     5761 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     5821 gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta
     5881 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     5941 agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt
     6001 aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat tgttacaaca
     6061 ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc
     6121 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     6181 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     6241 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     6301 ggccaagaag ggcggaaagt ccaaattgta aaatgtaact gtattcagcg atgacgaaat
     6361 tcttagctat tgtaatatta tatgcaaatt gatgaatggt aattttgtaa ttgtgggtca
     6421 ctgtactatt ttaacgaata ataaaatcag gtataggtaa ctaaaaagga attcgagctc
     6481 gaattgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     6541 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     6601 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     6661 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     6721 catctatgtt actagatctc tag
//
