LOCUS       sequence-308062-       10993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Novarch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Chien MP, Brinks D, Testa-Silva G, Tian H, Phil Brooks F 3rd, Adam 
            Y, Bloxham W, Gmeiner B, Kheifets S, Cohen AE
  TITLE     Photoactivated voltage imaging in tissue with an 
            archaerhodopsin-derived reporter.
  JOURNAL   Sci Adv. 2021 May 5;7(19). pii: 7/19/eabe3216. doi: 
            10.1126/sciadv.abe3216. Print 2021 May.
  PUBMED    33952514
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-05-5"; volume: "7(19). pii: 7/19/eabe3216. doi"; pages: 
            " 10.1126/sciadv.abe3216. Print 2021 May"
COMMENT     Sequence Label: MPC020: CamKII CMV_NovArch_citrine
FEATURES             Location/Qualifiers
     source          1..10993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..85)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        257..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        638..836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     791..811
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             854..1034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1081..1206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1699..1932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2117..2161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2459..2576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2621..3909
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3922..4680
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILASGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAARERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVIGLGTETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             join(4750..4752,4753..5463)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4792..4813)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5053..5072)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5400..5421
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5704..6292
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5757..5777)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6175..6186)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6295..6311)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6296..6312)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6817..6997
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7023..7040)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7029..7253
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7299..7727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7386..7405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7596..7617
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7736..7756)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7741..8070
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7921..8056
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7983..8002
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8118..8165
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8184..8558
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8688..8809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8725..8744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8779..8798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8858..8874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8858..8874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8871..8893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8882..8898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8906..8912,8913..8930,8931..8936))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8951..8972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9089..9106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9260..9848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9340..9359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10019..10810,10811..10879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10642..10661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10880..10984)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
       61 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
      121 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
      181 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      241 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      301 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
      841 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1021 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     1081 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     1141 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1201 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1261 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1321 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1381 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1441 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1501 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1561 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1621 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1681 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1741 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1801 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1861 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1921 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1981 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     2041 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     2101 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     2161 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2221 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2281 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2341 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2401 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2461 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2521 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2581 tttattacag ggacagcaga gatccagttt ggttaattaa cattatggcc ttaggtcact
     2641 tcatctccat ggggttcttc ttctgatttt ctagaaaatg agatgggggt gcagagagct
     2701 tcctcagtga cctgcccagg gtcacatcag aaatgtcaga gctagaactt gaactcagat
     2761 tactaatctt aaattccatg ccttgggggc atgcaagtac gatatacaga aggagtgaac
     2821 tcattagggc agatgaccaa tgagtttagg aaagaagagt ccagggcagg gtacatctac
     2881 accacccgcc cagccctggg tgagtccagc cacgttcacc tcattatagt tgcctctctc
     2941 cagtcctacc ttgacgggaa gcacaagcag aaactgggac aggagcccca ggagaccaaa
     3001 tcttcatggt ccctctggga ggatgggtgg ggagagctgt ggcagaggcc tcaggagggg
     3061 ccctgctgct cagtggtgac agataggggt gagaaagcag acagagtcat tccgtcagca
     3121 ttctgggtct gtttggtact tcttctcacg ctaaggtggc ggtgtgatat gcacaatggc
     3181 taaaaagcag ggagagctgg aaagaaacaa ggacagagac agaggccaag tcaaccagac
     3241 caattcccag aggaagcaaa gaaaccatta cagagactac aagggggaag ggaaggagag
     3301 atgaattagc ttcccctgta aaccttagaa cccagctgtt gccagggcaa cggggcaata
     3361 cctgtctctt cagaggagat gaagttgcca gggtaactac atcctgtctt tctcaaggac
     3421 catcccagaa tgtggcaccc actagccgtt accatagcaa ctgcctcttt gccccactta
     3481 atcccatccc gtctgttaaa agggccctat agttggaggt gggggaggta ggaagagcga
     3541 tgatcacttg tggactaagt ttgttcgcat ccccttctcc aaccccctca gtacatcacc
     3601 ctgggggaac agggtccact tgctcctggg cccacacagt cctgcagtat tgtgtatata
     3661 aggccagggc aaagaggagc aggttttaaa gtgaaaggca ggcaggtgtt ggggaggcag
     3721 ttaccggggc aacgggaaca gggcgtttcg gaggtggttg ccatggggac ctggatgctg
     3781 acgaaggctc gcgaggctgt gagcagccac agtgccctgc tcagaagccc caagctcgtc
     3841 agtcaagccg gttctccgtt tgcactcagg agcacgggca ggcgagtggc ccctagttct
     3901 gggggcagcg ggggatccac catggtaagt atcgctctgc aggctggtta cgacctactg
     3961 ggtgacggca gacctgaaac cctgtggctg ggcatcggca ctctgctgat gctgattgga
     4021 accttctact ttctggtccg cggatgggga gtcaccgata aggatgcccg ggaatattac
     4081 gctgtgacta tcctggcctc cggaatcgca tccgccgcat atctgtctat gttctttggt
     4141 atcgggctta ctgaggtgtc cgtcgggggc gaaatgttgg atatctatta tgccaggtac
     4201 gcccagtggc tgtttaccac cccacttctg ctgctgcacc tggcccttct cgctaaggtg
     4261 gatcgggtga ccatcggcac cctggtgggt gtggacgccc tgatgatcgt cactggcctc
     4321 atcggagcct tgagccacac ggccatagcc agatacagtt ggtggttgtt ctctacaatt
     4381 tgcatgatag tggtgctcta tgttctggct acatccctgc gatctgctgc aagggagcgg
     4441 ggccccgagg tggcatctac ctttaacacc ctgacagctc tggtcttggt gctgtggacc
     4501 gcttacccta tcctgtggat cataggcact gagggcgctg gcgtgatagg cctgggcacc
     4561 gaaactctgc tgtttatggt gttggacgtg actgccaagg tcggctttgg ctttatcctg
     4621 ttgagatccc gggctattct gggcgacacc gaggcaccag aacccagtgc cggtgccgat
     4681 acgttgatga agagtagaat cacaagcgaa ggcgagtaca tccccctgga tcaaatagac
     4741 ataaatgtag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4801 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4861 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4921 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
     4981 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5041 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5101 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5161 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5221 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5281 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5341 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5401 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5461 aaatcaagga tcacctctga gggcgagtat atccctctgg atcagatcga cattaacgtc
     5521 ggtggaaaat cccgcataac ctctgagggc gagtacatcc ctctggatca aatcgatata
     5581 aacgtggggg gaaaaagtcg aatcacttct gagggcgagt atatacccct cgatcagatt
     5641 gacatcaatg tggggggctt ttgttatgag aatgaataag aattcgatat caagcttatc
     5701 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5761 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5821 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5881 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5941 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6001 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6061 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6121 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6181 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6241 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     6301 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     6361 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     6421 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6481 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     6541 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     6601 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     6661 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     6721 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     6781 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     6841 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6901 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6961 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     7021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7261 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7501 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7621 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7741 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7801 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7861 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7981 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     8041 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     8101 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     8161 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     8221 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     8281 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     8341 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     8401 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     8461 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     8521 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     8581 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     8641 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     8701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     8821 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8881 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8941 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9001 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9061 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10321 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10981 cgcgcacatt tcc
//
