LOCUS       sequence-308063-       10936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encoded soma targeted NovArch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Chien MP, Brinks D, Testa-Silva G, Tian H, Phil Brooks F 3rd, Adam 
            Y, Bloxham W, Gmeiner B, Kheifets S, Cohen AE
  TITLE     Photoactivated voltage imaging in tissue with an 
            archaerhodopsin-derived reporter.
  JOURNAL   Sci Adv. 2021 May 5;7(19). pii: 7/19/eabe3216. doi: 
            10.1126/sciadv.abe3216. Print 2021 May.
  PUBMED    33952514
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-05-5"; volume: "7(19). pii: 7/19/eabe3216. doi"; pages: 
            " 10.1126/sciadv.abe3216. Print 2021 May"
COMMENT     Sequence Label: MPC021: CamKII CMV_sNovArch_citrine (soma targ...
FEATURES             Location/Qualifiers
     source          1..10936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..88)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        260..639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..839
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     794..814
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             857..1037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1084..1209
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1702..1935
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2120..2164
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2462..2579
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2624..3912
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3922..3931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3928..4686
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILASGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAARERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVIGLGTETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             join(4693..4695,4696..5406)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4735..4756)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4996..5015)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5343..5364
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5650..6238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5703..5723)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6121..6132)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6241..6257)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6242..6258)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6763..6943
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6969..6986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6975..7199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7245..7673
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7332..7351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7542..7563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7682..7702)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7687..8016
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7867..8002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7929..7948
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8064..8111
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8130..8504
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8634..8755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8671..8690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8725..8744
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8804..8820)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8804..8820)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8817..8839)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8828..8844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8852..8858,8859..8876,8877..8882))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8897..8918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9035..9052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9206..9794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9286..9305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9965..10756,10757..10825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10588..10607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10826..10930)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
       61 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
      121 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
      181 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      241 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      481 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      661 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      781 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
      841 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      901 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      961 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1021 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     1081 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1141 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1201 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1261 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1321 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1381 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1441 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1501 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1561 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1621 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1681 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1741 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1801 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1861 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1921 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1981 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     2041 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     2101 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     2161 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     2221 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2281 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2341 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2401 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     2461 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2521 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2581 gggtttatta cagggacagc agagatccag tttggttaat taacattatg gccttaggtc
     2641 acttcatctc catggggttc ttcttctgat tttctagaaa atgagatggg ggtgcagaga
     2701 gcttcctcag tgacctgccc agggtcacat cagaaatgtc agagctagaa cttgaactca
     2761 gattactaat cttaaattcc atgccttggg ggcatgcaag tacgatatac agaaggagtg
     2821 aactcattag ggcagatgac caatgagttt aggaaagaag agtccagggc agggtacatc
     2881 tacaccaccc gcccagccct gggtgagtcc agccacgttc acctcattat agttgcctct
     2941 ctccagtcct accttgacgg gaagcacaag cagaaactgg gacaggagcc ccaggagacc
     3001 aaatcttcat ggtccctctg ggaggatggg tggggagagc tgtggcagag gcctcaggag
     3061 gggccctgct gctcagtggt gacagatagg ggtgagaaag cagacagagt cattccgtca
     3121 gcattctggg tctgtttggt acttcttctc acgctaaggt ggcggtgtga tatgcacaat
     3181 ggctaaaaag cagggagagc tggaaagaaa caaggacaga gacagaggcc aagtcaacca
     3241 gaccaattcc cagaggaagc aaagaaacca ttacagagac tacaaggggg aagggaagga
     3301 gagatgaatt agcttcccct gtaaacctta gaacccagct gttgccaggg caacggggca
     3361 atacctgtct cttcagagga gatgaagttg ccagggtaac tacatcctgt ctttctcaag
     3421 gaccatccca gaatgtggca cccactagcc gttaccatag caactgcctc tttgccccac
     3481 ttaatcccat cccgtctgtt aaaagggccc tatagttgga ggtgggggag gtaggaagag
     3541 cgatgatcac ttgtggacta agtttgttcg catccccttc tccaaccccc tcagtacatc
     3601 accctggggg aacagggtcc acttgctcct gggcccacac agtcctgcag tattgtgtat
     3661 ataaggccag ggcaaagagg agcaggtttt aaagtgaaag gcaggcaggt gttggggagg
     3721 cagttaccgg ggcaacggga acagggcgtt tcggaggtgg ttgccatggg gacctggatg
     3781 ctgacgaagg ctcgcgaggc tgtgagcagc cacagtgccc tgctcagaag ccccaagctc
     3841 gtcagtcaag ccggttctcc gtttgcactc aggagcacgg gcaggcgagt ggcccctagt
     3901 tctgggggca gcgggggatc cgccaccatg gtaagtatcg ctctgcaggc tggttacgac
     3961 ctactgggtg acggcagacc tgaaaccctg tggctgggca tcggcactct gctgatgctg
     4021 attggaacct tctactttct ggtccgcgga tggggagtca ccgataagga tgcccgggaa
     4081 tattacgctg tgactatcct ggcctccgga atcgcatccg ccgcatatct gtctatgttc
     4141 tttggtatcg ggcttactga ggtgtccgtc gggggcgaaa tgttggatat ctattatgcc
     4201 aggtacgccc agtggctgtt taccacccca cttctgctgc tgcacctggc ccttctcgct
     4261 aaggtggatc gggtgaccat cggcaccctg gtgggtgtgg acgccctgat gatcgtcact
     4321 ggcctcatcg gagccttgag ccacacggcc atagccagat acagttggtg gttgttctct
     4381 acaatttgca tgatagtggt gctctatgtt ctggctacat ccctgcgatc tgctgcaagg
     4441 gagcggggcc ccgaggtggc atctaccttt aacaccctga cagctctggt cttggtgctg
     4501 tggaccgctt accctatcct gtggatcata ggcactgagg gcgctggcgt gataggcctg
     4561 ggcaccgaaa ctctgctgtt tatggtgttg gacgtgactg ccaaggtcgg ctttggcttt
     4621 atcctgttga gatcccgggc tattctgggc gacaccgagg caccagaacc cagtgccggt
     4681 gccgatcctc cggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4741 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4801 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4861 tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac
     4921 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4981 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5041 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     5101 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     5161 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     5221 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     5281 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     5341 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5401 tacaagcagt cccagcccat cctcaacacc aaggagatgg ccccgcagag caagcctcca
     5461 gaggagctgg agatgagcag catgcccagc cccgtggccc ctctgcccgc acgcacggag
     5521 ggcgtcatcg acatgcggag catgtccagc attgacagct tcatcagctg tgccacggac
     5581 ttccctgaag ccaccagatt cttttgttat gagaatgaag tgtaagaatt cgatatcaag
     5641 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5701 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5761 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5821 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5881 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5941 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6001 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6061 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6121 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6181 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6241 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     6301 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     6361 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6421 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     6481 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     6541 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     6601 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     6661 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     6721 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     6781 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6841 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6901 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     6961 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7021 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7081 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7141 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     7201 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     7261 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7321 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7381 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7441 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7501 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7561 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     7621 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     7681 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7741 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7801 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7861 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7921 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     7981 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     8041 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     8101 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     8161 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     8221 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     8281 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     8341 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     8401 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     8461 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     8521 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     8581 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     8641 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8701 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8761 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8821 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8881 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8941 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9001 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10921 gggttccgcg cacatt
//
