LOCUS       sequence-308065-        9265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyclin inducible expression of human PHGDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Richard Possemato
  TITLE     Richard Possemato Lab plasmids
  JOURNAL   Richard Possemato Lab plasmids
  PUBMED    32446203
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Richard 
            Possemato Lab plasmids"
COMMENT     Sequence Label: pCW57.1 hPHGDH
FEATURES             Location/Qualifiers
     source          1..9265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1929..2046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2100..2414
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2179..2197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2215..2233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2251..2269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2286..2304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2387..2411
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4095..4605
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4524..4542
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4615..5211
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4615..4634)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5111..5131
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5212..5265
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5266..5883,5884..5889,5890..5925,5926..5928,5929..5964,
                     5965..5967,5968..6003,6004..6012)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6052..6072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6470..6481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6686..6919
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6991..7112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7028..7047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7082..7101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7152..7287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7287..7358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7359..7427,7428..8219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7577..7596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8390..8978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8879..8898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9132..9149
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggacaa ttctcgacct cgagacaaat ggcagtattc
     1921 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1981 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2041 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcgaat cgatatgtcg
     2101 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
     2161 gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     2221 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2281 gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     2341 acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
     2401 accgtcagat cgcctggaga attggctagc accggtgcaa tggcttttgc aaatctgcgg
     2461 aaagtgctca tcagtgacag cctggaccct tgctgccgga agatcttgca agatggaggg
     2521 ctgcaggtgg tggaaaagca gaacctcagt aaggaagaac ttatcgcgga gctgcaggac
     2581 tgtgaaggcc ttattgttcg ctctgccacc aaggtgaccg ctgatgtcat caacgcagct
     2641 gagaaactcc aggtggtggg cagggctggc acaggtgtgg acaatgtgga tctggaggcc
     2701 gcaacaagga agggcatctt ggttatgaac acccccaatg ggaacagcct cagtgccgca
     2761 gaactcactt gtggaatgat catgtgcctg gccaggcaga ttccccaggc gacggcttcg
     2821 atgaaggacg gcaaatggga gcggaagaag ttcatgggaa cagagctgaa tggaaagacc
     2881 ctgggaattc ttggcctggg caggattggg agagaggtag ctacccggat gcagtccttt
     2941 gggatgaaga ctatagggta tgaccccatc atttccccag aggtctcggc ctcctttggt
     3001 gttcagcagc tgcccctgga ggagatctgg cctctctgtg atttcatcac tgtgcacact
     3061 cctctcctgc cctccacgac aggcttgctg aatgacaaca cctttgccca gtgcaagaag
     3121 ggggtgcgtg tggtgaactg tgcccgtgga gggatcgtgg acgaaggcgc cctgctccgg
     3181 gccctgcagt ctggccagtg tgccggggct gcactggacg tgtttacgga agagccgcca
     3241 cgggaccggg ccttggtgga ccatgagaat gtcatcagct gtccccacct gggtgccagc
     3301 accaaggagg ctcagagccg ctgtggggag gaaattgctg ttcagttcgt ggacatggtg
     3361 aaggggaaat ctctcacggg ggttgtgaat gcccaggccc ttaccagtgc cttctctcca
     3421 cacaccaagc cttggattgg tctggcagaa gctctgggga cactgatgcg agcctgggct
     3481 gggtccccca aagggaccat ccaggtgata acacagggaa catccctgaa gaatgctggg
     3541 aactgcctaa gccccgcagt cattgtcggc ctcctgaaag aggcttccaa gcaggcggat
     3601 gtgaacttgg tgaacgctaa gctgctggtg aaagaggctg gcctcaatgt caccacctcc
     3661 cacagccctg ctgcaccagg ggagcaaggc ttcggggaat gcctcctggc cgtggccctg
     3721 gcaggcgccc cttaccaggc tgtgggcttg gtccaaggca ctacacctgt actgcagggg
     3781 ctcaatggag ctgtcttcag gccagaagtg cctctccgca gggacctgcc cctgctccta
     3841 ttccggactc agacctctga ccctgcaatg ctgcctacca tgattggcct cctggcagag
     3901 gcaggcgtgc ggctgctgtc ctaccagact tcactggtgt cagatgggga gacctggcac
     3961 gtcatgggca tctcctcctt gctgcccagc ctggaagcgt ggaagcagca tgtgactgaa
     4021 gccttccagt tccacttcta accttggagc tcactggtcc ctgcctctgg ggcttttctg
     4081 aacaattcgg atccggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     4141 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     4201 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     4261 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     4321 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     4381 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     4441 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     4501 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     4561 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagcaatt caccatgacc
     4621 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4681 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     4741 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4801 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4861 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4921 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4981 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5041 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5101 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5161 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cgaaggtaga
     5221 ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc tagactggac
     5281 aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg
     5341 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     5401 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     5461 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     5521 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     5581 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     5641 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     5701 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     5761 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     5821 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     5881 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     5941 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     6001 ctccccgggt aactaagtaa ggatcgatcc aagatatcgt attcttaact atgttgctcc
     6061 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6121 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6181 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6241 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6301 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6361 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6421 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6481 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6541 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg tttcgcctcg
     6601 gcgtccggac tagaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6661 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6721 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6781 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6841 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6901 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6961 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7021 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7081 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7141 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7201 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7261 agtagtgagg aggctttttt ggaggccttt caaatatgta tccgctcatg agacaataac
     7321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9061 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9181 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9241 aagcgggcag tgagcgcaac gcaat
//
