LOCUS       sequence-30807-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(94-312)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(812..830)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      826..873
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgaaatat tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gtgccgcgcg gcagccatat gtcatcttct gtcccttccc
      121 agaaaaccta ccagggcagc tacggtttcc gtctgggctt cttgcattct gggacagcca
      181 agtctgtgac ttgcacgtac tcccctgccc tcaacaagat gttttgccaa ctggccaaga
      241 cctgccctgt gcagctgtgg gttgattcca cacccccgcc cggcacccgc gtccgcgcca
      301 tggccatcta caagcagtca cagcacatga cggaggttgt gaggcgctgc ccccaccatg
      361 agcgctgctc agatagcgat ggtctggccc ctcctcagca tcttatccga gtggaaggaa
      421 atttgcgtgt ggagtatttg gatgacagaa acacttttcg acatagtgtg gtggtgccct
      481 atgagccgcc tgaggttggc tctgactgta ccaccatcca ctacaactac atgtgtaaca
      541 gttcctgcat gggcggcatg aaccggaggc ccatcctcac catcatcaca ctggaagact
      601 ccagtggtaa tctactggga cggaacagct ttgaggtgcg tgtttgtgcc tgtcctggga
      661 gagaccggcg cacagaggaa gagaatctcc gcaagaaagg ggagcctcac cacgagctgc
      721 ccccagggag cactaagcga gcactgccca acaacaccta aggatccggc tgctaacaaa
      781 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      841 ggggcctcta aacgggtctt gaggggtttt ttgctgaagg aggaactata tccggatatc
      901 ccgcaagagg cccggcagta ccggcataac cagc
//
