LOCUS       sequence-308079-        1150 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of proteins with a C-terminal thermostable GFP 
            (TGP) tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D
  TITLE     An improved fluorescent tag and its nanobodies for membrane protein 
            expression, stability assay, and purification.
  JOURNAL   Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z.
  PUBMED    33303987
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-12-10"; volume: "3(1):753. doi"; pages: " 
            10.1038/s42003-020-01478-z"
COMMENT     Sequence Label: pBTSG
FEATURES             Location/Qualifiers
     source          1..1150
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          133..265
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     248..267
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      325..334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             373..396
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1090..1116
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
ORIGIN
        1 ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag ttaaattgct
       61 aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
      121 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      181 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      241 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaagga
      301 atcccggtcc gaagcgcgcg ggccgccacc atgggatcca agcttagcgg ccgcgaattc
      361 ctcgagggta ccctggaagt gctgtttcag ggcccgggcg gtagtggagg ttccgcgagt
      421 gtcattaagc cggagatgaa aattaagttg cgtatggagg gggccgtgaa tgggcacaaa
      481 ttcgttattg aaggggaggg gattggaaag ccgtatgagg gtacgcaaac cttggactta
      541 acagtagaag aaggggcacc attgcctttt tcttatgata ttttgactcc ggcgttccaa
      601 tacgggaacc gtgctttcac aaagtatccc gaagatatcc ctgactattt caagcaagca
      661 tttcccgagg gttacagttg ggagagaagt atgacctatg aggatcaagg catatgtatt
      721 gctacctctg acattactat ggagggagac tgcttcttct atgagattcg tttcgatgga
      781 acaaactttc ctcctaacgg gccagtgatg cagaaaaaga cgttgaagtg ggaaccgtcc
      841 acagaaaaaa tgtatgtcga ggacggcgtt ctaaagggag acgtggaaat ggccttgcta
      901 ttggaagggg gtggacacta tcgttgtgat ttcaaaacga cctacaaggc caaaaaagat
      961 gtgagactac cggacgccca cgaggttgac catcgtattg aaattttaag tcacgacaag
     1021 gactataata aagtaagact gtacgaacac gcggaagcac gttacagcgg aggtggaagt
     1081 ggtggtggcc atcaccatca ccatcaccat caccattaaa gcctgcagtc tcgacaagct
     1141 taatcaacct
//
