LOCUS       sequence-30811-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(82-360)
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..83
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             99..113
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tgggaattcc tctgaatatt ttgtttactt taagaaggag atataccatg ggccatcatc
       61 atcatcatca tcatcatcat cacagcagcg gccatatcga cgacgacgac aagcatatgc
      121 cggcggcccc tgcaccagcc ccctcctggc ccctgtcatc ttctgtccct tcccagaaaa
      181 cctaccaggg cagctacggt ttccgtctgg gcttcttgca ttctgggaca gccaagtctg
      241 tgacttgcac gtactcccct gccctcaaca agatgttttg ccaactggcc aagacctgcc
      301 ctgtgcagct gtgggttgat tccacacccc cgcccggcac ccgcgtccgc gccatggcca
      361 tctacaagca gtcacagcac atgacggagg ttgtgaggcg ctgcccccac catgagcgct
      421 gctcagatag cgatggtctg gcccctcctc agcatcttat ccgagtggaa ggaaatttgc
      481 gtgtggagta tttggatgac agaaacactt ttcgacatag tgtggtggtg ccctatgagc
      541 cgcctgaggt tggctctgac tgtaccacca tccactacaa ctacatgtgt aacagttcct
      601 gcatgggcgg catgaaccgg aggcccatcc tcaccatcat cacactggaa gactccagtg
      661 gtaatctact gggacggaac agctttgagg tgcgtgtttg tgcctgtcct gggagagacc
      721 ggcgcacaga ggaagagaat ctccgcaaga aaggggagcc tcaccacgag ctgcccccag
      781 ggagcactaa gcgagcactg cccaacaaca ccagctcctc tccccagcca aagaagaaac
      841 cactggatgg agaatatttc acccttcaga tccgtgggcg tgagcgcttc gagatgttcc
      901 gagagctgaa tgaggccttg gaactcagga tgcccaggct gggaaggagc cagggtgagg
      961 atccggctgt aaaccaaa
//
