LOCUS       sequence-308115-        3976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector based on the iOn integration-coupled 
            transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with 
            an MCS to clone-in genes of interest and express it from a CAG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Kumamoto T, Maurinot F, Barry-Martinet R, Vaslin C, 
            Vandormael-Pournin S, Le M, Lerat M, Niculescu D, Cohen-Tannoudji M,
            Rebsam A, Loulier K, Nedelec S, Tozer S, Livet J
  TITLE     Direct Readout of Neural Stem Cell Transgenesis with an 
            Integration-Coupled Gene Expression Switch.
  JOURNAL   Neuron. 2020 Aug 19;107(4):617-630.e6. doi: 
            10.1016/j.neuron.2020.05.038. Epub 2020 Jun 18.
  PUBMED    32559415
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.05.038"; journalName: "Neuron"; date: 
            "2020-08-19- 19"; volume: "107"; issue: "4"; pages: "617-630.e6"
COMMENT     Sequence Label: iOn-CAG∞MCS
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        23..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        405..680
                     /label=chicken beta-actin promoter
     intron          681..1689
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1613..1635
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1697..1716
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     repeat_region   1754..1788
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     repeat_region   2072..2134
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(2221..2809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2301..2320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2980..3771,3772..3840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3603..3622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3841..3945)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatat catttaaatg tcgacattga ttattgacta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tgggtcgagg tgagccccac
      421 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
      481 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg
      541 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
      601 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
      661 agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg
      721 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
      781 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt
      841 ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg ccctttgtgc gggggggagc
      901 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc
      961 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga
     1021 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc
     1081 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc ggtcgggctg
     1141 taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1201 gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     1261 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     1321 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     1381 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct ggcggagccg aaatctggga
     1441 ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga
     1501 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc
     1561 tcggggctgc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1621 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1681 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1741 ttctcgcgat taaccctaga aagatagtct gcgtaaaatt gacgcatgca tcaattgtaa
     1801 ctgcggttta aactccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     1861 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     1921 ctcattttat taggaaagga cagtgagagt ggcaccttcc agggtcaagg aaggcacggg
     1981 ggaggggcaa acaacagatg gctggcaact agaaggcact aaagtcggat cctcgagtgg
     2041 ccacctaggc ccgggctaat cacttaatta accctagaaa gataatcata ttgtgacgta
     2101 cgttaaagat aatcatgcgt aaaattgacg catgtccgga gccgtagata tcggaaagaa
     2161 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2221 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2281 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2341 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2401 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2461 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2521 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2581 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2641 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2701 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2761 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2821 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2881 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2941 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3001 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3061 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3121 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3181 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3241 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3301 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3361 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3421 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3481 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3541 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3601 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3661 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3721 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3781 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3841 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3901 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3961 agtgccacct gacgtc
//
