LOCUS       sequence-308116-        4203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector based on the LiOn integration-coupled translational switch 
            (Kumamoto et al bioRxiv 2019) expressing a functional Cre 
            recombinase from a CMV promoter upon action of the piggyBac 
            transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Kumamoto T, Maurinot F, Barry-Martinet R, Vaslin C, 
            Vandormael-Pournin S, Le M, Lerat M, Niculescu D, Cohen-Tannoudji M,
            Rebsam A, Loulier K, Nedelec S, Tozer S, Livet J
  TITLE     Direct Readout of Neural Stem Cell Transgenesis with an 
            Integration-Coupled Gene Expression Switch.
  JOURNAL   Neuron. 2020 Aug 19;107(4):617-630.e6. doi: 
            10.1016/j.neuron.2020.05.038. Epub 2020 Jun 18.
  PUBMED    32559415
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.05.038"; journalName: "Neuron"; date: 
            "2020-08-19- 19"; volume: "107"; issue: "4"; pages: "617-630.e6"
COMMENT     Sequence Label: LiOn-CMV∞Cre
FEATURES             Location/Qualifiers
     source          1..4203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        311..514
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     465..485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             551..571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     repeat_region   856..890
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1133..1254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1170..1189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1224..1243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1270..1293)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     repeat_region   2040..2102
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    2214..2325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2410..2998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2490..2509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3169..3960,3961..4029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3792..3811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4030..4134)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgcacgtg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      361 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      421 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      481 gcgtgtacgg tgggaggtct atataagcag agctcgaatt cggatttaaa tcgtttaaac
      541 caccatggtg cccaagaaga agaggaaagt ctccaacctg ctgactgtgc accaaaacct
      601 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt
      661 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc
      721 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt
      781 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca
      841 cctgggccag cttaacccta gaaagatagt ctgcgtaaaa ttgacgcatg cattaagcca
      901 tggcttcccg ccggcggtgg cggcgcagga tgatggcacg ctgcccatgt cttgtgccca
      961 ggagagcggg atggaccgtc accctgcagc ctgtgcttct gctaggatca atgtgtaggc
     1021 ggccgcgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1081 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     1141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcgcga
     1261 ggatccctac ttgtcgtcat cgtctttgta gtcgtcccca tcctcgagca gcctcaccat
     1321 ggccccagtc tcagagtcca ggtttctgat gtagttcatc actatgttca cattggtcca
     1381 gccaccagcc tgcatgattt cagggatgga cacaccagcc ctggccatgt ccctggcagc
     1441 acccactctg gcagagtggc cagaccaggc caggtatctc tgcccagagt catccttggc
     1501 accatagatc aggcggtggg tggcctcaaa gatcccttcc agggcccggg tggacagttg
     1561 ggaggtggca gaaggggcag ccacaccatt ctttctgacc cggcagaaca ggtagttgtt
     1621 ggggtcatca gccacaccag acacagagat ccatctctcc accagcttgg taacccccag
     1681 ggacagggcc ttctccacac cagctgtgga caccagggtc ttggtcctgc caatgtggat
     1741 cagcattctc ccaccatcgg tgcgggagat gtccttcact ctgattctgg caatttcggc
     1801 aatgcgcagc agggtgttgt aggcaatgcc caggaaggcc aggttcctga tgtcctggca
     1861 tctgtcagag ttctccatca gggatctgac ttggtcaaag tcagtgcgtt caaaggccag
     1921 ggcctgcttg gctctctccc cagcatccac attctccttt ctgattctcc tcatcaccag
     1981 ggacacagca ttggagtcag aagggcgagg caggccagat ctcctgtgca gcatgttaac
     2041 cctagaaaga taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa aattgacgca
     2101 tgtcagctac ttaactagtc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     2161 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     2221 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     2281 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagaccgg taattgatat
     2341 cggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4141 tccccgaaaa gtgccacctg acgtcccaat gatatcggat ttaaatccaa gcttgccgtc
     4201 gac
//
