LOCUS       sequence-308143-        9673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of human TMPRSS11D..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-TMPRSS11D-puro
FEATURES             Location/Qualifiers
     source          1..9673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2582..2606)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2614..2655
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2706..3294
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2759..2779)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3177..3188)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3366..3599
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3677..3798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3714..3733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3768..3787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3838..3973
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3993..4012)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3994..4012)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4022..4039)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4022..4038)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4031..4053)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4180..4635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4267..4286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4477..4498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4661..4765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4766..4834,4835..5626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4984..5003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5797..6385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6286..6305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6539..6556
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6673..6694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6709..6714,6715..6732,6733..6739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6747..6763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6752..6774
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6771..6787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6771..6787
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6806..6826
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6808..6826
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6854..7080
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7081..7261
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7308..7433
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7926..8159
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8344..8388
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8619..8829
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8641..8837
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8688..8823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8869..9471
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8869..8888)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9365..9385
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9514..9631
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgtata ggccagcacg tgtaacttcg acttcaagat ttctgaatcc atatgtagta
     1381 tgtttcattg tcgtcgcagg ggtagtgatc ctggcagtca ccatagctct acttgtttac
     1441 tttttagctt ttgatcaaaa atcttacttt tataggagca gttttcaact cctaaatgtt
     1501 gaatataata gtcagttaaa ttcaccagct acacaggaat acaggacttt gagtggaaga
     1561 attgaatctc tgattactaa aacattcaaa gaatcaaatt taagaaatca gttcatcaga
     1621 gctcatgttg ccaaactgag gcaagatggt agtggtgtga gagcggatgt tgtcatgaaa
     1681 tttcaattca ctagaaataa caatggagca tcaatgaaaa gcagaattga gtctgtttta
     1741 cgacaaatgc tgaataactc tggaaacctg gaaataaacc cttcaactga gataacatca
     1801 cttactgacc aggctgcagc aaattggctt attaatgaat gtggggccgg tccagaccta
     1861 ataacattgt ctgagcagag aatccttgga ggcactgagg ctgaggaggg aagctggccg
     1921 tggcaagtca gtctgcggct caataatgcc caccactgtg gaggcagcct gatcaataac
     1981 atgtggatcc tgacagcagc tcactgcttc agaagcaact ctaatcctcg tgactggatt
     2041 gccacgtctg gtatttccac aacatttcct aaactaagaa tgagagtaag aaatatttta
     2101 attcataaca attataaatc tgcaactcat gaaaatgaca ttgcacttgt gagacttgag
     2161 aacagtgtca cctttaccaa agatatccat agtgtgtgtc tcccagctgc tacccagaat
     2221 attccacctg gctctactgc ttatgtaaca ggatggggcg ctcaagaata tgctggccac
     2281 acagttccag agctaaggca aggacaggtc agaataataa gtaatgatgt atgtaatgca
     2341 ccacatagtt ataatggagc catcttgtct ggaatgctgt gtgctggagt acctcaaggt
     2401 ggagtggacg catgtcaggg tgactctggt ggcccactag tacaagaaga ctcacggcgg
     2461 ctttggttta ttgtggggat agtaagctgg ggagatcagt gtggcctgcc ggataagcca
     2521 ggagtgtata ctcgagtgac agcctacctt gactggatta ggcaacaaac tgggatctag
     2581 aacccagctt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     2641 ggtctcgatt ctacgtagta atgaactagt accggttaag tcgacaatca acgcgttaag
     2701 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2761 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2821 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2881 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2941 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3001 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3061 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3121 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3181 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3241 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     3301 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3361 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     3421 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3481 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     3541 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     3601 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     3661 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3721 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3781 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     3841 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3901 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3961 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     4021 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     4081 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4141 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     4201 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4261 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4321 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4381 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4441 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4501 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4561 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4621 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4681 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4741 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4801 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4861 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4921 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4981 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5041 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5101 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5161 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5221 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5281 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5341 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5401 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5461 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5521 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5581 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5641 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5701 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5761 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5821 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5881 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5941 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6001 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6061 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6121 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6181 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6241 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6301 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6361 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6421 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6481 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6541 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6601 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6661 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6721 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6781 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     6841 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     6901 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     6961 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     7021 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     7081 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7141 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7201 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7261 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     7321 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     7381 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     7441 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     7501 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     7561 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     7621 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     7681 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     7741 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     7801 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     7861 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     7921 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     7981 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8041 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     8101 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     8161 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     8221 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     8281 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     8341 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     8401 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     8461 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     8521 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
     8581 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8641 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8701 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8821 gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac
     8881 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     8941 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     9001 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9061 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     9121 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9181 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9241 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9301 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     9361 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9421 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta
     9481 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     9541 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9601 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     9661 actttggcgc cgg
//
