LOCUS       sequence-308145-       11320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of human APN..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-APN-puro
FEATURES             Location/Qualifiers
     source          1..11320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4229..4253)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4261..4302
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4353..4941
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4406..4426)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4824..4835)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5013..5246
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5324..5445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5361..5380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5415..5434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5485..5620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5640..5659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5641..5659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5669..5686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5669..5685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5678..5700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5827..6282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5914..5933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6124..6145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6308..6412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6413..6481,6482..7273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6631..6650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7444..8032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7933..7952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8186..8203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8320..8341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8356..8361,8362..8379,8380..8386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8394..8410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8399..8421
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8418..8434
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8418..8434
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8453..8473
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8455..8473
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8501..8727
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8728..8908
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8955..9080
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9573..9806
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9991..10035
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10266..10476
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10288..10484
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10335..10470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10516..11118
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10516..10535)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11012..11032
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11161..11278
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatggcca agggcttcta tatttccaag tccctgggca tcctggggat cctcctgggc
     1381 gtggcagccg tgtgcacaat catcgcactg tcagtggtgt actcccagga gaagaacaag
     1441 aacgccaaca gctcccccgt ggcctccacc accccgtccg cctcagccac caccaacccc
     1501 gcctcggcca ccaccttgga ccaaagtaaa gcgtggaatc gttaccgcct ccccaacacg
     1561 ctgaaacccg attcctaccg ggtgacgctg agaccgtacc tcacccccaa tgacaggggc
     1621 ctgtacgttt ttaagggctc cagcaccgtc cgtttcacct gcaaggaggc cactgacgtc
     1681 atcatcatcc acagcaagaa gctcaactac accctcagcc aggggcacag ggtggtcctg
     1741 cgtggtgtgg gaggctccca gccccccgac attgacaaga ctgagctggt ggagcccacc
     1801 gagtacctgg tggtgcacct caagggctcc ctggtgaagg acagccagta tgagatggac
     1861 agcgagttcg agggggagtt ggcagatgac ctggcgggct tctaccgcag cgagtacatg
     1921 gagggcaatg tcagaaaggt ggtggccact acacagatgc aggctgcaga tgcccggaag
     1981 tccttcccat gcttcgatga gccggccatg aaggccgagt tcaacatcac gcttatccac
     2041 cccaaggacc tgacagccct gtccaacatg cttcccaaag gtcccagcac cccacttcca
     2101 gaagacccca actggaatgt cactgagttc cacaccacgc ccaagatgtc cacgtacttg
     2161 ctggccttca ttgtcagtga gttcgactac gtggagaagc aggcatccaa tggtgtcttg
     2221 atccggatct gggcccggcc cagtgccatt gcggcgggcc acggcgatta tgccctgaac
     2281 gtgacgggcc ccatccttaa cttctttgct ggtcattatg acacacccta cccactccca
     2341 aaatcagacc agattggcct gccagacttc aacgccggcg ccatggagaa ctggggactg
     2401 gtgacctacc gggagaactc cctgctgttc gaccccctgt cctcctccag cagcaacaag
     2461 gagcgggtgg tcactgtgat tgctcatgag ctggcccacc agtggttcgg gaacctggtg
     2521 accatagagt ggtggaatga cctgtggctg aacgagggct tcgcctccta cgtggagtac
     2581 ctgggtgctg actatgcgga gcccacctgg aacttgaaag acctcatggt gctgaatgat
     2641 gtgtaccgcg tgatggcagt ggatgcactg gcctcctccc acccgctgtc cacacccgcc
     2701 tcggagatca acacgccggc ccagatcagt gagctgtttg acgccatctc ctacagcaag
     2761 ggcgcctcag tcctcaggat gctctccagc ttcctgtccg aggacgtatt caagcagggc
     2821 ctggcgtcct acctccacac ctttgcctac cagaacacca tctacctgaa cctgtgggac
     2881 cacctgcagg aggctgtgaa caaccggtcc atccaactcc ccaccaccgt gcgggacatc
     2941 atgaaccgct ggaccctgca gatgggcttc ccggtcatca cggtggatac cagcacgggg
     3001 accctttccc aggagcactt cctccttgac cccgattcca atgttacccg cccctcagaa
     3061 ttcaactacg tgtggattgt gcccatcaca tccatcagag atggcagaca gcagcaggac
     3121 tactggctga tagatgtaag agcccagaac gatctcttca gcacatcagg caatgagtgg
     3181 gtcctgctga acctcaatgt gacgggctat taccgggtga actacgacga agagaactgg
     3241 aggaagattc agactcagct gcagagagac cactcggcca tccctgtcat caatcgggca
     3301 cagatcatta atgacgcctt caacctggcc agtgcccata aggtccctgt cactctggcg
     3361 ctgaacaaca ccctcttcct gattgaagag agacagtaca tgccctggga ggccgccctg
     3421 agcagcctga gctacttcaa gctcatgttt gaccgctccg aggtctatgg ccccatgaag
     3481 aactacctga agaagcaggt cacacccctc ttcattcact tcagaaataa taccaacaac
     3541 tggagggaga tcccagaaaa cctgatggac cagtacagcg aggttaatgc catcagcacc
     3601 gcctgctcca acggagttcc agagtgtgag gagatggtct ctggcctttt caagcagtgg
     3661 atggagaacc ccaataataa cccgatccac cccaacctgc ggtccaccgt ctactgcaac
     3721 gctatcgccc agggcgggga ggaggagtgg gacttcgcct gggagcagtt ccgaaatgcc
     3781 acactggtca atgaggctga caagctccgg gcagccctgg cctgcagcaa agagttgtgg
     3841 atcctgaaca ggtacctgag ctacaccctg aacccggact taatccggaa gcaggacgcc
     3901 acctctacca tcatcagcat taccaacaac gtcattgggc aaggtctggt ctgggacttt
     3961 gtccagagca actggaagaa gctttttaac gattatggtg gtggctcgtt ctccttctcc
     4021 aacctcatcc aggcagtgac acgacgattc tccaccgagt atgagctgca gcagctggag
     4081 cagttcaaga aggacaacga ggaaacaggc ttcggctcag gcacccgggc cctggagcaa
     4141 gccctggaga agacgaaagc caacatcaag tgggtgaagg agaacaagga ggtggtgctc
     4201 cagtggttca cagaaaacag caaatagaac ccagctttct tgtacaaagt ggttgatatc
     4261 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtacc
     4321 ggttaagtcg acaatcaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     4381 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4441 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4501 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4561 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4621 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4681 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4741 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4801 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4861 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4921 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     4981 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5041 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5101 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5161 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5221 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     5281 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5461 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5581 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5641 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     5701 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5761 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5821 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5881 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5941 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6001 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6061 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6121 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6181 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6241 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6301 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6361 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6421 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6481 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6541 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6601 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6661 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6721 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6781 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6841 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6901 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6961 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7021 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7081 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7141 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7201 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7261 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7321 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7381 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7561 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8101 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8161 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8221 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8281 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8341 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8401 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     8461 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     8521 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     8581 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     8641 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     8701 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     8761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8881 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8941 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     9001 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     9061 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     9121 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     9181 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     9241 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     9301 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     9361 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     9421 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     9481 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9541 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9601 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9661 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9721 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9781 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9841 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9901 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9961 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    10021 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    10081 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    10141 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    10201 aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10261 ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc
    10321 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10441 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta
    10501 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
    10561 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
    10621 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
    10681 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc
    10741 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
    10801 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
    10861 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
    10921 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
    10981 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
    11041 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
    11101 aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa
    11161 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
    11221 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
    11281 ggtttattac agggacagca gagatccact ttggcgccgg
//
