LOCUS       sequence-308152-        9215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of human CTSL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-CTSL-blast
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2327..2351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2359..2400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2451..3039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2504..2524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2922..2933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3111..3344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3422..3543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3459..3478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3513..3532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3583..3718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3738..3757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3739..3757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3767..3784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3767..3783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3776..3798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3925..4380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4012..4031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4222..4243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4406..4510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4511..4579,4580..5371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4729..4748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5542..6130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6031..6050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6284..6301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6418..6439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6454..6459,6460..6477,6478..6484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6492..6508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6497..6519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6516..6532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6516..6532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6551..6571
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6553..6571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6599..6825
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6826..7006
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7053..7178
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7671..7904
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8089..8133
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8364..8574
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8386..8582
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8433..8568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      8604..8613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8610..9008
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9056..9173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgaatc ctacactcat ccttgctgcc ttttgcctgg gaattgcctc agctactcta
     1381 acatttgatc acagtttaga ggcacagtgg accaagtgga aggcgatgca caacagatta
     1441 tacggcatga atgaagaagg atggaggaga gcagtgtggg agaagaacgt gaagatgatt
     1501 gaactgcaca atcaggaata cagggaaggg aaacacagct tcacaatggc catgaacgcc
     1561 tttggagaca tgaccagtga agaattcagg caggtgatga atggctttca aaaccgtaag
     1621 cccaggaagg ggaaagtgtt ccaggaacct ctgttttatg aggcccccag atctgtggat
     1681 tggagagaga aaggctacgt gactcctgtg aagaatcagg gtcagtgtgg ttcttgttgg
     1741 gcttttagtg ctactggtgc tcttgaagga cagatgttcc ggaaaactgg gaggcttatc
     1801 tcactgagtg agcagaatct ggtagactgc tctgggcctc aaggcaatga aggctgcaat
     1861 ggtggcctaa tggattatgc tttccagtat gttcaggata atggaggcct ggactctgag
     1921 gaatcctatc catatgaggc aacagaagaa tcctgtaagt acaatcccaa gtattctgtt
     1981 gctaatgaca ccggctttgt ggacatccct aagcaggaga aggccctgat gaaggcagtt
     2041 gcaactgtgg ggcccatttc tgttgctatt gatgcaggtc atgagtcctt cctgttctat
     2101 aaagaaggca tttattttga gccagactgt agcagtgaag acatggatca tggtgtgctg
     2161 gtggttggct acggatttga aagcacagaa tcagataaca ataaatattg gctggtgaag
     2221 aacagctggg gtgaagaatg gggcatgggt ggctacgtaa agatggccaa agaccggaga
     2281 aaccattgtg gaattgcctc agcagccagc taccccactg tgtagaaccc agctttcttg
     2341 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2401 tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc
     2461 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2521 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2581 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2641 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2701 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2761 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2821 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2881 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2941 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3001 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     3061 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     3121 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     3181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     3361 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     3421 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3541 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     3601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3721 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     3781 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3841 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3901 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3961 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4021 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4081 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4141 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4201 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4261 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4321 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4501 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4561 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4621 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4681 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4741 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4801 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4861 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4921 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4981 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5041 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5101 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5161 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5221 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5281 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5341 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6181 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6301 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6361 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6421 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     6481 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     6541 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     6601 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     6661 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     6721 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     6781 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     6841 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6901 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6961 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7021 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     7081 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     7141 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     7201 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     7261 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     7321 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     7381 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     7441 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     7501 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     7561 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     7621 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     7681 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     7741 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     7801 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     7861 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     7921 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     7981 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8041 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8101 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8161 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8221 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8281 acccacctcc caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt
     8341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt
     8401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8521 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8581 aaaagctttc tagaggtacc gtggccacca tggccaagcc tttgtctcaa gaagaatcca
     8641 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     8701 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     8761 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     8821 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     8881 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     8941 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     9001 agggctaagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9061 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9121 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9181 attacaggga cagcagagat ccactttggc gccgg
//
